LOCUS       sequence-41674-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2,
            Klf4 and Myc for iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: TetO-FUW-OSKM
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(146..170)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(167..205)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(238..256,257..279,280..298,299..321,322..340,341..363,
                     364..382,383..405,406..424,425..447,448..466,467..489,
                     490..508)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    565..682
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgacccatc acccccacct ggtgggggtg agaaggcgaa gtctgaagcc aggtgtccag
       61 ccatggcgaa ttcggggccg cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa
      121 aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct ctgcttatat
      181 aggcctccca ccgtacacgc ctacctcgac ccgggtaccg agctcgactt tcacttttct
      241 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
      301 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
      361 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
      421 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      481 tttcactttt ctctatcact gatagggagt ggtaaactcg agatctaacc aaactggatc
      541 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
      601 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
      661 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc agaattggcg
      721 cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      781 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      841 ataatggtga atatccctgc ctaactctat tcactata
//
