LOCUS       sequence-41675-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-4SA(S109)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..47
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgtcagaat tgatctacca tggnactaca aagacgatga cgacaagctt gcggccgcga
       61 attcaagctt agccaccatg gactacaaag acgatgacga taaagcaagg ctcgaatcgg
      121 tacctaagga tcccgggcag cagccgccgc ctcaaccggc cccccagggc caagggcagc
      181 cgccttcgca gcccccgcag gggcagggcc cgccgtccgg acccgggcaa ccggcacccg
      241 cggcgaccca ggcggcgccg caggcacccc ccgccgggca tcagatcgtg cacgtccgcg
      301 gggacgcgga gaccgacctg gaggcgctct tcaacgccgt catgaacccc aagacggcca
      361 acgtgcccca gaccgtgccc atgaggctcc ggaagctgcc cgactccttc ttcaagccgc
      421 cggagcccaa atcccactcc cgacaggcca gtactgatgc aggcactgca ggagccctga
      481 ctccacagca tgttcgagct catgccgctc cagctgctct gcagttggga gctgtttctc
      541 ctgggacact gacccccact ggagtagtct ctggcccagc agctacaccc acagctcagc
      601 atcttcgaca ggctgctttt gagatacctg atgatgtacc tctgccagca ggttgggaga
      661 tggcaaagac atcttctggt cagagatact tcttaaatca catcgatcag acaacaacat
      721 ggcaggaccc caggaaggcc atgctgtccc agatgaacgt cacagccccc accagtccac
      781 cagtgcagca gaatatgatg aactcggctt caggtcctct tcctgatgga tgggaacaag
      841 ccatg
//
