LOCUS       sequence-416755-        9246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Takada A, Asano T, Nakahama KI, Ono T, Nakata T, Ishii T
  TITLE     Development of an optogenetics tool, Opto-RANK, for control of 
            osteoclast differentiation using blue light.
  JOURNAL   Sci Rep. 2024 Jan 19;14(1):1749. doi: 10.1038/s41598-024-52056-w.
  PUBMED    38242937
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-01-19"; volume: "14(1):1749. doi"; pages: " 
            10.1038/s41598-024-52056-w"
COMMENT     Sequence Label: CRY2clust-mCherry-RANK
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        366..643
                     /label=chicken beta-actin promoter
     intron          644..1660
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1584..1606
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1668..1687
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3310..4017
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3455..3473)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3723..3742
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5307..5895
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5360..5380)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5778..5789)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6034..6155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6071..6090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6125..6144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6162..6617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6299..6320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6511..6530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6644..6748
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6750..7107
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6750..6941
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6773..6793)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6958..7093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7142..7936
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7142..7936
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7196..7215)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7806..7825
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8124..8143)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8168..8215
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8544..9132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9033..9052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      421 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      481 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      541 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      601 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      661 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      721 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      781 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      841 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
      901 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
      961 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     1021 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1081 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1141 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1201 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1261 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1321 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1381 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     1441 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     1501 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     1561 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1621 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1681 tattgtgctg tctcatcatt ttggcaaaga attgatttat cgctagttaa ttaagctagc
     1741 gccaccatga agatggacaa aaagaccatc gtctggtttc ggagagattt gagaatagaa
     1801 gataatcccg cgctcgccgc cgcggcccac gagggttccg tcttccccgt tttcatttgg
     1861 tgtcctgaag aagaaggcca gttttatccc ggaagggcct ctaggtggtg gatgaagcaa
     1921 agtctggccc atcttagcca gtcactgaaa gcactgggca gtgatcttac cctgatcaag
     1981 acacacaata ccatctctgc catcctcgac tgcatcaggg tgaccggcgc aacgaaagtc
     2041 gtgtttaacc acctgtacga tccagttagt ctggtgcgcg accacactgt gaaggagaag
     2101 ctggtggaac gggggatcag tgtgcagagc tacaacgggg accttctgta cgagccatgg
     2161 gagatctatt gcgagaaagg gaaaccgttc acctccttca acagttactg gaagaaatgt
     2221 ttggatatgt caatagagtc cgttatgttg ccccctccct ggagactgat gccgattact
     2281 gctgctgcag aggccatctg ggcctgctcc atcgaggaac tcggtctgga aaatgaagca
     2341 gaaaagccaa gcaatgcact tctcactaga gcctggagcc ccggctggtc taatgccgac
     2401 aagctgctta acgagttcat cgaaaaacaa ctgattgact acgcgaagaa ctccaagaaa
     2461 gtggtaggta actcaactag cttgctctct ccatatctcc attttggcga gatttctgtc
     2521 cgccatgtat ttcagtgcgc tcggatgaaa cagattatct gggctcgcga taaaaacagc
     2581 gaaggcgaag aaagcgccga tctgttcctg cgagggatcg gacttcggga atactcccgg
     2641 tatatatgtt tcaactttcc attcacacac gagcagagtc tgttgtccca cctcaggttc
     2701 ttcccctggg acgccgatgt cgacaaattc aaggcatgga gacagggaag gacaggatac
     2761 ccactcgtgg atgctggcat gagagagctc tgggctacag gctggatgca caaccgcatc
     2821 cgggtaatcg tgtcctcatt tgctgtcaag tttctgctcc tgccttggaa atggggaatg
     2881 aagtactttt gggataccct tctcgacgcc gacttggagt gtgacattct gggatggcaa
     2941 tatattagcg ggtcaattcc tgacggccat gagttggaca ggttggacaa tccggccttg
     3001 cagggagcta agtatgatcc cgaaggagag tatattcgac agtggctccc cgagctggcc
     3061 cgacttccta cggagtggat tcaccatcct tgggacgcac cactgacagt gctcaaggca
     3121 agcggggtgg agctgggcac caattacgct aagcctatag ttgatataga tacagcacgc
     3181 gagctgctgg ctaaagcgat ctctcgcact cgggaggcgc agattatgat cggtgctgcc
     3241 gcccgggatc caccggatct agataacgaa ttcgatagtg ctggtagtgc tggtagtgct
     3301 ggtactagta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3361 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3421 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3481 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3541 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3601 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3661 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3721 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3781 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     3841 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3901 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3961 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc
     4021 ggactcagat ctcgagtggg tggatctgga ggttcatact acaggaaggg agggaaagcg
     4081 ctgacagcta atttgtggaa ttgggtcaat gatgcttgca gtagtctaag tggaaataag
     4141 gagtcctcag gggaccgttg tgctggttcc cactcggcaa cctccagtca gcaagaagtg
     4201 tgtgaaggta tcttactaat gactcgggag gagaagatgg ttccagaaga cggtgctgga
     4261 gtctgtgggc ctgtgtgtgc ggcaggtggg ccctgggcag aagtcagaga ttctaggacg
     4321 ttcacactgg tcagcgaggt tgagacgcaa ggagacctgt cgaggaagat tcccacagag
     4381 gatgagtaca cggaccggcc ctcgcagcct tcgactggtt cactgctcct aatccagcag
     4441 ggaagcaaat ctataccccc attccaggag cccctggaag tgggggagaa cgacagttta
     4501 agccagtgtt tcaccgggac tgaaagcacg gtggattctg agggctgtga cttcactgag
     4561 cctccgagca gaactgactc tatgcccgtg tcccctgaaa agcacctgac aaaagaaata
     4621 gaaggtgaca gttgcctccc ctgggtggtc agctccaact caacagatgg ctacacaggc
     4681 agtgggaaca ctcctgggga ggaccatgaa ccctttccag ggtccctgaa atgtggacca
     4741 ttgccccagt gtgcctacag catgggcttt cccagtgaag cagcagccag catggcagag
     4801 gcgggagtac ggccccagga cagggctgat gagaggggag cctcagggtc cgggagctcc
     4861 cccagtgacc agccacctgc ctctgggaac gtgactggaa acagtaactc cacgttcatc
     4921 tctagcgggc aggtgatgaa cttcaagggt gacatcatcg tggtgtatgt cagccagacc
     4981 tcgcaggagg gcccgggttc cgcagagccc gagtcggagc ccgtgggccg ccctgtgcag
     5041 gaggagacgc tggcacacag agactccttt gcgggcaccg cgccgcgctt ccccgacgtc
     5101 tgtgccaccg gggctgggct gcaggagcag ggggcacccc ggcagaagga cgggacatcg
     5161 cggccggtgc aggagcaggg tggggcgcag acttcactcc atacccaggg gtccggacaa
     5221 tgtgcagaag gaggttcagg tggatctggt acctgacctc agcggccgca ctagtttgag
     5281 tcgacggcgc gccatagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5341 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5401 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5461 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5521 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5581 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5641 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5701 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5761 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5821 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5881 gggccgcctc cccgcatcga taccgtggat ccaaggccgc gactctagat cataatcagc
     5941 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6001 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6061 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6121 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     6181 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     6241 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6301 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6361 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6421 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6481 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6541 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6601 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6661 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6721 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     6781 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6841 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6901 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6961 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7021 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7081 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     7141 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     7201 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     7261 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     7321 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     7381 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     7441 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     7501 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     7561 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     7621 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     7681 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     7741 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     7801 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     7861 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     7921 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     7981 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8041 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8101 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     8161 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     8221 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     8281 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     8341 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     8401 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8461 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9241 atgcat
//
