LOCUS       sequence-416767-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing scramble of shRNA targeting Ctnnd2 N terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-scrCtnnd2.1-GFP
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    717..842
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             889..1069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1070..1296)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1324..1344)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1324..1342)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1363..1379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1363..1379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1376..1398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1387..1403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1411..1417,1418..1435,1436..1441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1456..1477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1594..1611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1765..2353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1845..1864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2524..3315,3316..3384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3147..3166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3385..3489)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3515..3970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3652..3673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3864..3883
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4097..4119
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4111..4128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4112..4128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4138..4157
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4138..4156
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4177..4312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4352..4473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4363..4382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4417..4436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4545..4778
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     4967..4983
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4968..4984
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4987..5575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5093..5104
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5502..5522
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5631..5664
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(5684..6397,6398..6400,6401..6403))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5687..6397,6398..6400,6401..6403))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5729..5750)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6078..6097
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6337..6358
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6400..6409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6459..6478)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6486..7502
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6540..6562)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7503..7780)
                     /label=chicken beta-actin promoter
     enhancer        7782..8085
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8452..8569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(8666..8914)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8724..8743)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8894..8914)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(9212..9256)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9441..9450,1..224)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
       61 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
      121 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
      181 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
      241 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
      301 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
      361 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
      421 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
      481 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
      541 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
      601 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
      661 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
      721 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
      781 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
      841 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
      901 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
      961 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     1021 gttagccaga gagctcccag gctcagatct ggtctaacca gagagacccg tttatgtatc
     1081 gagctaggca cttaaataca atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc
     1141 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca
     1201 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca
     1261 tgttgcaaga ctacaagagt attgcataag actacattaa gcttgcagct ccagcttttg
     1321 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     1381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3481 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     3541 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3601 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3661 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3721 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3781 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3841 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3901 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3961 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4021 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     4081 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     4141 tacgactcac tatagggcga attgggtacg tccctaggcc tccaaaaaag cctcctcact
     4201 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     4261 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     4321 ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt tggacaaacc
     4381 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4441 tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt
     4501 tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag agattttcca
     4561 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     4621 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     4681 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag
     4741 cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
     4801 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtacct gaggtgtgac
     4861 tggaaaaccc acctcctcct cctcttgtgc ttctagccag gcacaatcag cattggtagc
     4921 tgctgtattg ctacttgtga ttgctccatg tttttctagg tctcgatcga ggtcgacggt
     4981 atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     5041 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     5101 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     5161 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     5221 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     5281 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     5341 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     5401 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     5461 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     5521 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga
     5581 taagcttgat atcgaattgt acctagtgga accggaaccc ttaaacatgt ataacttcgt
     5641 ataatgtatg ctatacgaag ttattaggtc cctcgacgaa ttcctacttg tacagctcgt
     5701 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     5761 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     5821 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     5881 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     5941 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     6001 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     6061 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     6121 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     6181 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     6241 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     6301 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     6361 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtagcg
     6421 ctagcggccg cgaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc
     6481 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
     6541 gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc
     6601 cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg
     6661 ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag
     6721 ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc
     6781 tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag
     6841 ccgccggcgc tccgggggcc gccgcgcccc tcccccgagc cctccccggc ccgaggcggc
     6901 cccgccccgc ccggcacccc cacctgccgc caccccccgc ccggcacggc gagccccgcg
     6961 ccacgccccg tacggagccc cgcacccgaa gccgggccgt gctcagcaac tcggggaggg
     7021 gggtgcaggg ggggttgcag cccgaccgac gcgcccacac cccctgctca cccccccacg
     7081 cacacacccc gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg
     7141 cccccggccg cgctcccctc gcgcacactg cggagcgcac aaagccccgc gccgcgcccg
     7201 cagcgctcac agccgccggg cagcgcggag ccgcacgcgg cgctccccac gcacacacac
     7261 acgcacgcac cccccgagcc gctccccccg cacaaagggc cctcccggag cccctcaagg
     7321 ctttcacgca gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta attacagccc
     7381 ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg
     7441 ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact
     7501 cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa
     7561 ggaaactttc ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg
     7621 ccccgcccct cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc
     7681 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
     7741 gagatgggga gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact
     7801 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     7861 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     7921 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     7981 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     8041 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     8101 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtgag acgtgcggct
     8161 tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac cttcccgact taggggcgga
     8221 gcaggaagcg tcgccggggg gcccacaagg gtagcggcga agatccgggt gacgctgcga
     8281 acggacgtga agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc
     8341 ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg caaccccaac
     8401 cccctcgagc cgcggccaaa gtggatctct gctgtccctg taataaaccc gaaaattttg
     8461 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
     8521 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa
     8581 tactgccatt tgtctcgagg tcgagaattc aaaaagcctt gggagttaag tgatgtctcg
     8641 agacatcact taactcccaa ggcccggtgt ttcgtccttt ccacaagata tataaagcca
     8701 agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt
     8761 ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata
     8821 tctttgtgtt tacagtcaaa ttaattccaa ttatctctct aacagccttg tatcgtatat
     8881 gcaaatatga aggaatcatg ggaaataggc cctcggtgaa gggggcggcc gctcgaggct
     8941 agtctcgtga tcgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc
     9001 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     9061 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     9121 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     9181 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     9241 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     9301 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     9361 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     9421 cagagcaacc ccaaatcccc aggagctgtt
//
