LOCUS       sequence-416768-        9447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing shRNA targeting Ctnnd2 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-shCtnnd2.2-GFP
FEATURES             Location/Qualifiers
     source          1..9447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..276
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    769..894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             941..1121
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1122..1348)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1376..1396)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1376..1394)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1415..1431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1415..1431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1428..1450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1439..1455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1463..1469,1470..1487,1488..1493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1508..1529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1646..1663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1817..2405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1897..1916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2576..3367,3368..3436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3199..3218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3437..3541)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3567..4022)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3704..3725)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3916..3935
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4149..4171
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4163..4180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4164..4180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4190..4209
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4190..4208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4229..4364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4404..4525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4415..4434)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4469..4488
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4597..4830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     5019..5035
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5020..5036
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5039..5627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5145..5156
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5554..5574
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5683..5716
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(5736..6449,6450..6452,6453..6455))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5739..6449,6450..6452,6453..6455))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5781..5802)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6130..6149
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6389..6410
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6452..6461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6511..6530)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6538..7554
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6592..6614)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        7831..8134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8501..8618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(8715..8963)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8773..8792)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8943..8963)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(9261..9305)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
       61 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
      121 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
      181 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
      241 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
      301 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
      361 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
      421 atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc
      481 taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac
      541 tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc
      601 cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc
      661 tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct
      721 taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca
      781 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
      841 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
      901 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
      961 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     1021 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttctc tagttagcca
     1081 gagagctccc aggctcagat ctggtctaac cagagagacc cgtttatgta tcgagctagg
     1141 cacttaaata caatatctct gcaatgcggc aattcagtgg ttcgtccaat ccatgtcaga
     1201 cccgtctgtt gccttcctaa taaggcacga tcgtaccacc ttacttccac caatcggcat
     1261 gcacggtgct ttttctctcc ttgtaaggca tgttgctaac tcatcgttac catgttgcaa
     1321 gactacaaga gtattgcata agactacatt aagcttgcag ctccagcttt tgttcccttt
     1381 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3541 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     3601 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3661 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3721 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3781 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3841 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3901 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3961 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4021 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4081 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4141 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     4201 actatagggc gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg
     4261 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     4321 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     4381 gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4441 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4501 cattataagc tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt
     4561 ataaatactg aataataaga tgacatgaac tactactgct agagattttc cacactgact
     4621 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     4681 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     4741 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt
     4801 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     4861 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac ctgaggtgtg actggaaaac
     4921 ccacctcctc ctcctcttgt gcttctagcc aggcacaatc agcattggta gctgctgtat
     4981 tgctacttgt gattgctcca tgtttttcta ggtctcgatc gaggtcgacg gtatcgatgc
     5041 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     5101 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     5161 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     5221 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     5281 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     5341 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     5401 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     5461 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     5521 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     5581 agaataccag tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg
     5641 atatcgaatt gtacctagtg gaaccggaac ccttaaacat gtataacttc gtataatgta
     5701 tgctatacga agttattagg tccctcgacg aattcctact tgtacagctc gtccatgccg
     5761 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     5821 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     5881 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     5941 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     6001 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     6061 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     6121 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     6181 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     6241 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     6301 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     6361 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     6421 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtag cgctagcggc
     6481 cgcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg
     6541 taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg
     6601 ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca
     6661 gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc
     6721 gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg
     6781 gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct
     6841 ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc
     6901 gctccggggg ccgccgcgcc cctcccccga gccctccccg gcccgaggcg gccccgcccc
     6961 gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg cgccacgccc
     7021 cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag gggggtgcag
     7081 ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca cgcacacacc
     7141 ccgcacgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac cgcccccggc
     7201 cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc
     7261 acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac acacgcacgc
     7321 accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa ggctttcacg
     7381 cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc ccggaggaga
     7441 agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg
     7501 gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcag cgcgcagcga ctcccgcccg
     7561 ccgcgcgctt cgctttttat agggccgccg ccgccgccgc ctcgccataa aaggaaactt
     7621 tcggagcgcg ccgctctgat tggctgccgc cgcacctctc cgcctcgccc cgccccgccc
     7681 cccgcccccc cccccccccc ccgccccccc cccccccccc cccgccccca tcgctgcaca
     7741 aaataattaa aaaataaata aatacaaaat tgggggtggg gagggggggg agatggggag
     7801 agtgaagcag aacgtggggc tcacctcgac catggtaata gcgatgacta atacgtagat
     7861 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     7921 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     7981 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     8041 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     8101 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     8161 taatgacccc gtaattgatt actattaata actagtgaga cgtgcggctt ccgtttgtca
     8221 cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt aggggcggag caggaagcgt
     8281 cgccgggggg cccacaaggg tagcggcgaa gatccgggtg acgctgcgaa cggacgtgaa
     8341 gaatgtgcga gacccagggt cggcgccgct gcgtttcccg gaaccacgcc cagagcagcc
     8401 gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc aaccccaacc ccctcgagcc
     8461 gcggccaaag tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
     8521 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     8581 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
     8641 gtctcgaggt cgagaattca aaaacgcttt gtttactctc ttcatctcga gatgaagaga
     8701 gtaaacaaag cgccggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa
     8761 tactttcaag ttacggtaag catatgatag tccattttaa aacataattt taaaactgca
     8821 aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt
     8881 acagtcaaat taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa
     8941 ggaatcatgg gaaataggcc ctcggtgaag ggggcggccg ctcgaggcta gtctcgtgat
     9001 cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
     9061 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
     9121 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     9181 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     9241 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     9301 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     9361 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     9421 ttactccaac tagcattcca aggcaca
//
