LOCUS       sequence-416770-        8761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing scramble of shRNA targeting Cdh2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-scrCdh2.1 GFP
FEATURES             Location/Qualifiers
     source          1..8761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(164..175)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(284..300)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(285..301)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             490..723
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    795..916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(832..851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     886..905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      956..1091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1111..1130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1112..1130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1140..1157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1140..1156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1149..1171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1298..1753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1385..1404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1595..1616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1779..1883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1884..1952,1953..2744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2102..2121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2915..3503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3404..3423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3657..3674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3791..3812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3827..3832,3833..3850,3851..3857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3865..3881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3870..3892
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3889..3905
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3889..3905
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3924..3944
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3926..3944
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3972..4198
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4199..4379
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4426..4551
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5044..5277
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5462..5506
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5804..6052
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5804..5824
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5975..5994
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6149..6266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6633..6936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7467..7489
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7551..7570
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      7620..7629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7626..7628,7629..7631,7632..8345)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFRRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7671..7692)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7932..7951)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8279..8300
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(8365..8398)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(8454..8761,1..281)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8507..8527)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
       61 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
      121 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
      181 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
      241 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
      301 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
      361 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
      421 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
      481 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
      541 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      721 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
      781 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      901 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
      961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     1021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     1081 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     1141 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1201 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1261 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1741 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1801 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1861 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1921 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1981 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2041 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2101 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2161 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2221 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2281 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2341 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2401 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2461 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2521 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2581 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2641 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2701 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2761 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2821 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3481 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3541 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3601 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3661 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3721 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3781 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     3841 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     3901 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     3961 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     4021 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     4081 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     4141 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     4201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4381 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4441 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4501 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4561 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4621 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4681 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     4741 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     4801 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     4861 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     4921 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     4981 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     5041 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     5101 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5161 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5221 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5281 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5341 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5401 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5461 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5521 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5581 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5641 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     5701 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     5761 gtatcgatca cgagactagc ctcgagcggc cgcccccttc accgagggcc tatttcccat
     5821 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
     5881 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     5941 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     6001 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgggaactc
     6061 ttaatcgcaa tattgctcga gcaatattgc gattaagagt tctttttgaa ttctcgacct
     6121 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     6181 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     6241 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     6301 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     6361 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     6421 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     6481 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     6541 cggacgtgac aaacggaagc cgcacgtctc actagttatt aatagtaatc aattacgggg
     6601 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     6661 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     6721 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     6781 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     6841 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     6901 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     6961 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     7021 ttattttgtg cagcgatggg ggcggggggg gggggggggg ggcgcggggg gggggggggg
     7081 gggggggggg gggggggggg gggggggggg gggggggggg gggggcgggc gggggggggg
     7141 gggggggggc gggggggggg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     7201 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     7261 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     7321 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     7381 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     7441 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     7501 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     7561 ggttattgtg ctgtctcatc attttggcaa agaattcgcg gccgctagcg ctaccggtcg
     7621 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     7681 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     7741 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     7801 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt ccgccgctac cccgaccaca
     7861 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     7921 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     7981 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8041 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     8101 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     8161 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     8221 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     8281 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     8341 agtaggaatt cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac
     8401 atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac
     8461 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     8521 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     8581 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8641 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     8701 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     8761 c
//
