LOCUS       sequence-416780-       11176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses all necessary components of Type I-C CRISPR-Cas system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Lammens E-M, Volke DC, Schroven K, Voet M, Kerremans A, Lavigne R, 
            Hendrix H
  TITLE     A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for 
            Pseudomonas with efficient vector curing.
  JOURNAL   Microbiol Spectr. 2023 Nov 17:e0270723. doi: 
            10.1128/spectrum.02707-23.
  PUBMED    37975669
REFERENCE   2  (bases 1 to 11176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23."
COMMENT     Sequence Label: pCas3-Km
FEATURES             Location/Qualifiers
     source          1..11176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      69..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(283..302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             294..1109
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(join(1231..1236,1237..1253,1254..1259))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      1300..1651
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1665..2498
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPARRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(2499..3347)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(3421..4257)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        4403..4521
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     regulatory      6454..6463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7630..7641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     oriT            10442..10550
                     /note="incP origin of transfer"
     rep_origin      complement(join(10620..11176,1..32))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10700..10719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
       61 ctgacgctac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
      361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
      421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
      481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
      541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
      601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
      661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
      721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
      781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
      841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
      901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
      961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcc gcgcgttgtc
     1141 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     1201 ctgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaat
     1261 tcgcgccgct ggtgccgctg gttggacgcc aagggtgaat ccgcctcgat accctgatta
     1321 ctcgcttcct gcgccctctc aggcggcgat aggggactgg taaaacgggg attgcccaga
     1381 cgcctccccc gccccttcag gggcacaaat gcggccccaa cggggccacg tagtggtgcg
     1441 ttttttgcgt ttccaccctt ttcttccttt tcccttttaa accttttagg acgtctacag
     1501 gccacgtaat ccgtggcctg tagagtttaa aaagggacgg atttgttgcc attaagggac
     1561 ggatttgttg ttaagaaggg acggatttgt tgttgtaaag ggacggattt gttgtattgt
     1621 gggacgcaga tacagtgtcc ccttatacac aaggaatgtc gaacgtggcc tcacccccaa
     1681 tggtttacaa aagcaatgcc ctggtcgagg ccgcgtatcg cctcagtgtt caggaacagc
     1741 ggatcgttct ggcctgtatt agccaggtga agaggagcga gcctgtcacc gatgaagtga
     1801 tgtattcagt gacggcggag gacatagcga cgatggcggg tgtccctatc gaatcttcct
     1861 acaaccagct caaagaagcg gccctgcgcc tgaaacggcg ggaagtccgg ttaacccaag
     1921 agcccaatgg caaggggaaa agaccgagtg tgatgattac cggctgggtg caaacaatca
     1981 tctaccggga gggtgagggc cgtgtagaac tcaggttcac caaagacatg ctgccgtacc
     2041 tgacggaact caccaaacag ttcaccaaat acgccttggc tgacgtggcc aagatggaca
     2101 gcacccacgc gatcaggctt tacgagctgc tcatgcaatg ggacagcatc ggccagcgcg
     2161 aaatagaaat tgaccagctg cgaaagtggt ttcaactgga aggccggtat ccctcgatca
     2221 aggacttcaa gttgcgagtg cttgatccag ccgtgacgca gatcaacgag cacagcccgc
     2281 tacaggtgga gtgggcgcag cgaaagaccg ggcgcaaggt cacacatctg ttgttcagtt
     2341 ttggaccgaa gaagcccgcc aaggcggtgg gtaaggcccc agcgaggcgc aaggccggga
     2401 agatttcaga tgctgagatc gcgaaacagg ctcgccctgg tgagacatgg gaagcggccc
     2461 gcgctcgact aacccagatg ccgctggatc tggcctagtt aatctttctg cgaattgaga
     2521 tgacgccact ggctgggcgt catcccggtt tcccgggtaa acaccaccga aaaatagtta
     2581 ctatcttcaa agccacattc ggtcgaaata tcactgatta acaggcggct atgctggaga
     2641 agatattgcg catgacacac tctgacctgt cgcagatatt gattgatggt cattccagtc
     2701 tgctggcgaa attgctgacg caaaacgcgc tcactgcacg atgcctcatc acaaaattta
     2761 tccagcgcaa agggactttt caggctagcc gccagccggg taatcagctt atccagcaac
     2821 gtttcgctgg atgttggcgg caacgaatca ctggtgtaac gatggcgatt cagcaacatc
     2881 accaactgcc cgaacagcaa ctcagccatt tcgttagcaa acggcacatg ctgactactt
     2941 tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca tccccatgct acctaagcgc
     3001 cagtgtggtt gccctgcgct ggcgttaaat cccggaatcg ccccctgcca gtcaagattc
     3061 agcttcagac gctccgggca ataaataata ttctgcaaaa ccagatcgtt aacggaagcg
     3121 taggagtgtt tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat gcgataaggg
     3181 cgatcgttga gtacatgcag gccattaccg cgccagacaa tcaccagctc acaaaaatca
     3241 tgtgtatgtt cagcaaagac atcttgcgga taacggtcag ccacagcgac tgcctgctgg
     3301 tcgctggcaa aaaaatcatc tttgagaagt tttaactgat gcgccaccgt ggctacctcg
     3361 gccagagaac gaagttgatt attcgcaata tggcgtacaa atacgttgag aagattcgcg
     3421 ttattgcaga aagccatccc gtccctggcg aatatcacgc ggtgaccagt taaactctcg
     3481 gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg cgatgtcagt
     3541 aacgctggcc tcgctgtggc gtagcagatg tcgggctttc atcagtcgca ggcggttcag
     3601 gtatcgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcagtga
     3661 aagagaaaat tgatccgcca cggcatccca attcacctca tcggcaaaat ggtcctccag
     3721 ccaggccaga agcaagttga gacgtgatgc gctgttttcc aggttctcct gcaaactgct
     3781 tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg tcgagggtaa
     3841 atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgcacct gctgcaatac
     3901 gctgtggtta acgcgccagt gagacggata ctgcccatcc agctcttgtg gcagcaactg
     3961 attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat tggtcagaca
     4021 cagattatcg gtatgttcat acagatgccg atcatgatcg cgtacgaaac agaccgtgcc
     4081 accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat gttcgacaat
     4141 cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc
     4201 tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact
     4261 ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt cttaccttaa
     4321 attttcgacg gaaaaccacg taaaaaacgt cgatttttca agatacagcg tgaattttca
     4381 ggaaatgcgg tgagcatcac atcaccacaa ttcagcaaat tgtgaacatc atcacgttca
     4441 tctttccctg gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc gcgaattcag
     4501 gcgcttttta gactggtcgt aatgaaattc aactaggtcg cgccccgcac gggcgcgtgg
     4561 attgaaacga gacctctctg gacaaaggtc tcgtcgcccg gcaaaaccgg gcgtggattg
     4621 aaacctagtg ctctgcagga attcctcgag aagggagcgc acctatggac gcggaggcta
     4681 gcgatactca cttttttgct cactccacct taaaggcaga tcgcagcgat tggcagcctc
     4741 tggtcgagca tctacaggct gttgcccgtt tggcaggaga gaaggctgcc ttcttcggcg
     4801 gcggtgaatt agctgctctt gctggtctgt tgcatgactt gggtaaatac actgacgagt
     4861 ttcagcggcg tattgcgggt gatgccatcc gtgtcgatca ctctactcgc ggggccatac
     4921 tggcggtaga acgctatggc gcgctaggtc aattgctagc ctacggcatc gctggccacc
     4981 atgccgggtt ggccaatggc cgcgaggctg gtgagcgaac tgccttggtc gaccgcctga
     5041 aaggggttgg gctgccacgg ttattggagg ggtggtgcgt ggaaatcgtg ctacccgagc
     5101 gccttcaacc accgccacta aaagcgcgcc tggaaagagg tttctttcag ttggcctttc
     5161 ttggccggat gctcttttcc tgcttggttg atgcggatta tctagatacc gaagccttct
     5221 accaccgcgt cgaaggacgg cgctcccttc gcgagcaagc gcggccgacc ttggccgagt
     5281 tacgcgcagc ccttgatcgg catctgactg agttcaaggg agatacgccg gtcaaccgcg
     5341 ttcgcgggga gatattggcc ggcgtgcgcg gcaaggcgag cgaacttccc gggctgtttt
     5401 ctctcacagt gcccacagga ggcggcaaga ccctggcctc tctggctttc gccctggatc
     5461 acgctctagc tcatgggctg cgccgggtga tctacgtgat tcccttcact agcatcgtcg
     5521 agcagaacgc tgcggtattc cgtcgtgcac tcggggcctt aggcgaagag gcggtgctgg
     5581 agcatcacag cgccttcgtt gatgaccgcc ggcagagcct ggaggccaag aagaaactga
     5641 acctagcgat ggagaactgg gacgcgccta tcgtggtgac cactgcagtg cagttcttcg
     5701 aaagcctgtt tgccgaccgt ccagcccagt gccgcaagct acacaacatc gccggcagcg
     5761 tggtgattct tgacgaggca cagaccctac cgctcaagct gttgcggccc tgcgttgccg
     5821 cccttgatga actggcgctc aactaccgtt gtagcccagt tctctgtact gccacgcagc
     5881 cagcgcttca atcgccggat ttcatcggtg ggctgcagga cgtacgtgag ctggcgcccg
     5941 agccgcagcg gctgttccgg gagttggtgc gggtacgaat acggacattg ggcccgctcg
     6001 aagatgcggc cttgactgag cagatcgcca ggcgtgaaca agtgctgtgc atcgtcaaca
     6061 atcgacgcca ggcccgtgcg ctctatgagt cgcttgccga gttgcccggt gcccgccatc
     6121 tcaccaccct gatgtgcgcc aagcaccgta gcagcgtgct ggccgaggtg cgccagatgc
     6181 tcaaaaaggg ggagccctgt cgcctggtgg ccacctcgct gatcgaggcc ggtgtggatg
     6241 tggattttcc cgtggtactg cgtgccgagg ctggattgga ttccatcgcc caggccgcgg
     6301 gacgctgcaa tcgcgaaggc aagcggccgc tggccgaaag cgaggtgctg gtgttcgccg
     6361 cggccaattc tgactgggcg ccacccgagg aactcaagca gttcgcccag gccgcccgcg
     6421 aagtgatgcg cctgcacccg gatgattgcc tgtccatggc ggccatcgag cggtattttc
     6481 gcatactgta ctggcagaag ggcgcggagg agttggatgc gggtaacctg ctcggcctga
     6541 ttgagagagg ccggctcgat ggcctgccct acgagacttt ggccaccaag ttccgcatga
     6601 tcgacagcct tcaactgccg gtgatcatcc catttgatga cgaggccaga gcagccctgc
     6661 gcgagctgga gttcgccgac ggctgcgccg ccatcgcccg tcgcctgcag ccatatctgg
     6721 tgcagatgcc acgcaagggt tatcaggcat tgcgggaagc cggtgcgatc caggcggcgg
     6781 caggtacgcg ttatggtgag cagtttatgg cgttggtcaa ccctgatctg tatcaccacc
     6841 aattcgggtt gcactgggat aatccggcct ttgtcagcag cgagcggcta tgttggtagt
     6901 cgggacgcgc aacagcggcc tggcctggat gatgtgaaag ggagggccga tggcctacgg
     6961 aattcgctta atggtctggg gcgagcgtgc ctgcttcacc cgcccggaaa tgaaggtgga
     7021 acgcgtctct tacgatgcga tcacgccgtc cgccgcgcgc ggcattctcg aggctatcca
     7081 ctggaagccg gcgattcgct gggtggtgga tcgcattcaa gtgcttaagc cgatccgctt
     7141 cgaatccatc cggcgcaacg aggtcggcgg caagctgtcc gctgtcagcg tcggtaaggc
     7201 aatgaaggcc gggcgtacta atggtctggt gaatctggtc gaggaggatc gccagcagcg
     7261 cgcgactact ctgctgcgcg atgtctccta tgtcatcgag gcgcatttcg agatgactga
     7321 cagggctggc gccgacgata cggtgggcaa gcatctggat atcttcaacc gtcgcgcacg
     7381 gaaggggcag tgcttccata caccctgcct aggcgtgcgc gagtttccgg ccagttttcg
     7441 gttgctggaa gagggcagtg ccgagcctga agtcgatgcc tttctgcgcg gcgagcgtga
     7501 tctgggctgg atgctgcatg acattgactt cgccgatggc atgaccccgc acttcttccg
     7561 tgccctgatg cgcgatgggc tgatcgaggt gccggccttc agggcggcag aggacaaggc
     7621 atgatccttt cggccctcaa tgactattat cagcgactgc tggagcgggg tgaagcgaat
     7681 atctcaccct tcggctacag ccaagaaaag atcagttacg ccctgctgct gtccgcacaa
     7741 ggagagttgc tggacgtgca ggacattcgc ttgctctctg gcaagaagcc tcaacccagg
     7801 cttatgagtg tgccgcagcc ggagaagcgc acctcgggca tcaagtccaa cgtactgtgg
     7861 gacaagacca gctatgtgct gggtgttagt gccaagggcg gagagcgtac tcagcaggag
     7921 cacgagtcct tcaagacgct gcaccggcag atcttggttg gggaaggcga ccccggtctg
     7981 caggccttgc tccagttcct cgactgttgg cagccggagc agttcaagcc cccgctgttc
     8041 agcgaagcaa tgctcgacag caacttagtg ttccgcctag acggccaaca acgctatctg
     8101 cacgagactc cggcggccct ggcgttgcgt acccggctgt tggccgacgg cgacagccgc
     8161 gaggggctgt gcctagtctg cggccaacgt cagccgttgg cgcgcctgca tccagcggtc
     8221 aagggcgtca atggtgccca gagttcgggg gcttccatcg tctccttcaa cctcgacgct
     8281 ttttcctcct acggcaagag ccagggggaa aatgctccgg tgtccgaaca ggccgccttt
     8341 gcctacacca cggtgctcaa ccatttgttg cgtcgcgacg agcacaaccg ccagcgcctg
     8401 cagattggcg acgcgagtgt ggtgttctgg gcgcaggcgg atactcctgc tcaggtggcc
     8461 gccgccgagt cgaccttctg gaacctgctg gagccacccg cagatgatgg tcaggaagcg
     8521 gaaaagctgc gcggcgtgct ggatgctgtg gccacggggc ggcccttgca tgagctcgac
     8581 tcgctaatgg aggaaggcac ccgcattttt gtgttagggc tggcgcccaa tacctcgcga
     8641 ctgtccattc ggttctgggc agtcgatagc cttgcggtat tcacccagca tctggccgag
     8701 catttccggg atatgcacct tgagcctctg ccctggaaga cggagccggc catctggcgc
     8761 ttgctctatg ctaccgcgcc cagtcgtgac ggcagagcca agaccgaaga cttactccca
     8821 caactggccg gtgaaatgac ccgcgccatc ctgaccggca gccgctatcc gcgcagtttg
     8881 ctagccaacc tgatcatgcg catgcgtgcc gacggcgacg tctctggcat acgcgtcgcg
     8941 ctgtgcaagg ccgtgctcgc tcgcgaggca cgcctgagcg gcaaaattca ccaagaggag
     9001 ctacctatga gtctcgacaa ggacgccagc aaccccggct atcgcttggg gaggctgttc
     9061 gccgtgttgg aaggcgccca gcgcgcagcc ctgggcgaca gggtcaatgc cactatccgt
     9121 gaccgctact acggtgccgc gtccagcacg ccagccacgg ttttcccgat actgctgcgc
     9181 aacacacaaa accacttggc caagctgcgc aaggagaagc ccggactagc agtgaaccta
     9241 gagcgcgata taggcgaaat cattgacggt atgcagagcc aattcccgcg ttgcctgcgc
     9301 ctggaggacc agggacgctt tgctattggt tactaccaac aggcccaggc ccgtttcaac
     9361 cgtggccccg attccgtcga gtaaggagca gaagaatgac cgccatctcc aaccgctacg
     9421 agttcgttta cctctttgat gtcagcaatg gcaatcccaa tggcgacccg gatgctggca
     9481 acatgccgcg tctcgatccg gaaaccaacc aggggttggt cactgacgtt tgcctcaagc
     9541 gcaagatccg caactacgtc agcctggagc aggaaagtgc ccccggctat gccatctata
     9601 tgcaggaaaa atccgtgctg aataaccagc acaaacaggc ctacgaggcg ctcggtatcg
     9661 agtcagaggc aaagaaactg cccaaggacg aagccaaggc gcgcgaactg acctcttgga
     9721 tgtgcaagaa cttcttcgat gtgcgtgctt tcggggcggt gatgaccacc gagattaatg
     9781 ccggccaggt gcgtggaccg atccaactgg cattcgccac gtctatcgac ccggtattgc
     9841 ctatggaggt atccatcacc cgcatggcgg tgactaacga aaaggatttg gagaaggaac
     9901 gcaccatggg acgcaagcac atcgtgcctt acggcttgta ccgcgcccat ggtttcatct
     9961 ctgccaagtt ggccgagcga accggctttt ccgacgacga cttggaactg ctatggcgcg
    10021 ctttggccaa tatgttcgaa cacgaccgct cggcggcacg tggcgagatg gcagcgcgca
    10081 agttgatcgt cttcaagcat gagcatgcca tgggcaatgc acccgcccat gtgctgttcg
    10141 gcagcgttaa ggtcgagcga gtcgaggggg acgcagttac accagcacgc ggtttccagg
    10201 attaccgtgt cagcatcgat gcggaagctc tgcctcaggg cgtgagcgtg cgcgagtacc
    10261 tctagtttca ggtatcgata ccgtcgacgg ggattcctta aggtatactt tccgctgcat
    10321 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
    10381 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
    10441 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
    10501 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg ataagctagc
    10561 ttgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaggctc cctcgtgcgc
    10741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11161 ttttttgttt gcaagc
//
