LOCUS       sequence-416782-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing shRNA targeting Cdh10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-shCdh10.1 GFP
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    68..185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        552..855
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        857..1133
                     /label=chicken beta-actin promoter
     intron          1134..2150
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2074..2096
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2158..2177
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2227..2236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2233..2235,2236..2238,2239..2952)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2233..2235,2236..2238,2239..2949)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2278..2299)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2539..2558)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2886..2907
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2972..3005)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3061..3649
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3114..3134)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3532..3543)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3652..3668)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3653..3669)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3858..4091
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4163..4284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4200..4219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4254..4273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4324..4459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4479..4498)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4480..4498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4508..4525)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4508..4524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4517..4539)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4666..5121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4753..4772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4963..4984
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5147..5251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5252..5320,5321..6112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5470..5489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6283..6871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6772..6791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7025..7042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7159..7180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7195..7200,7201..7218,7219..7225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7233..7249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7238..7260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7257..7273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7257..7273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7292..7312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7294..7312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7340..7566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7567..7747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7794..7919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8412..8645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8830..8874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9172..9420
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9172..9192
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9343..9362
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatatagtct tcggttctca ttttttgaat tctcgacctc gagacaaatg gcagtattca
       61 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      121 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      181 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
      241 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
      301 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
      361 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
      421 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
      481 gcacgtctca ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      541 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      601 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      661 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      721 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      781 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      841 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
      901 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
      961 gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     1021 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     1081 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1141 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     1201 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     1261 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     1321 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     1381 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     1441 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     1501 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1561 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1621 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1681 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1741 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     1801 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1861 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1921 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1981 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     2041 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     2101 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     2161 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgcggcc gctagcgcta
     2221 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2281 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2341 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2401 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2461 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2521 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2581 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2641 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2701 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2761 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2821 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2881 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2941 ctgtacaagt aggaattcgt cgagggacct aataacttcg tatagcatac attatacgaa
     3001 gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat
     3061 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3121 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3181 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3241 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3301 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3361 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3421 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3481 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3541 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3601 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     3661 cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     3721 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     3781 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     3841 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     3901 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3961 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4021 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4081 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4141 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     4201 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4261 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     4321 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4381 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4441 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     4501 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4561 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4621 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     4681 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4741 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4801 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4861 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4921 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4981 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5041 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5101 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5161 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5221 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5281 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5341 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5401 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5461 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5521 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5581 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5641 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5701 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5761 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5821 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5881 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5941 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6001 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6061 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6121 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6181 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6421 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7021 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7081 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7141 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7201 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7261 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     7321 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     7381 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     7441 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     7501 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     7561 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7621 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7681 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7741 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7801 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     7861 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     7921 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     7981 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     8041 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     8101 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     8161 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     8221 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     8281 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     8341 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     8401 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     8461 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     8521 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     8581 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     8641 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     8701 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     8761 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     8821 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     8881 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     8941 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     9001 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     9061 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     9121 tctcgacggt atcgatcacg agactagcct cgagcggccg cccccttcac cgagggccta
     9181 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     9241 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     9301 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     9361 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     9421 ggatgagaac cgaagactat atactcgag
//
