LOCUS       sequence-416784-       11277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses all necessary components of Type I-C CRISPR-Cas system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Lammens E-M, Volke DC, Schroven K, Voet M, Kerremans A, Lavigne R, 
            Hendrix H
  TITLE     A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for 
            Pseudomonas with efficient vector curing.
  JOURNAL   Microbiol Spectr. 2023 Nov 17:e0270723. doi: 
            10.1128/spectrum.02707-23.
  PUBMED    37975669
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23."
COMMENT     Sequence Label: pCas3-Amp
FEATURES             Location/Qualifiers
     source          1..11277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     446..465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            615..723
                     /note="incP origin of transfer"
     CDS             3524..3535
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      4702..4711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6644..6762)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             6908..7744
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             7818..8666
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(8667..9500)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPARRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      9514..9865
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        join(9906..9911,9912..9928,9929..9934)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      complement(10036..10075)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(join(10079..10870,10871..10939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10940..11031)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(11104..11197)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(11234..11277,1..545)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
       61 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      421 gagagcgcac gagggagcct ccagggggaa acgcctggta tctttatagt cctgtcgggt
      481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      601 acaagctagc ttatcggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
      661 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
      721 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
      781 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
      841 ggttatgcag cggaaagtat accttaagga atccccgtcg acggtatcga tacctgaaac
      901 tagaggtact cgcgcacgct cacgccctga ggcagagctt ccgcatcgat gctgacacgg
      961 taatcctgga aaccgcgtgc tggtgtaact gcgtccccct cgactcgctc gaccttaacg
     1021 ctgccgaaca gcacatgggc gggtgcattg cccatggcat gctcatgctt gaagacgatc
     1081 aacttgcgcg ctgccatctc gccacgtgcc gccgagcggt cgtgttcgaa catattggcc
     1141 aaagcgcgcc atagcagttc caagtcgtcg tcggaaaagc cggttcgctc ggccaacttg
     1201 gcagagatga aaccatgggc gcggtacaag ccgtaaggca cgatgtgctt gcgtcccatg
     1261 gtgcgttcct tctccaaatc cttttcgtta gtcaccgcca tgcgggtgat ggatacctcc
     1321 ataggcaata ccgggtcgat agacgtggcg aatgccagtt ggatcggtcc acgcacctgg
     1381 ccggcattaa tctcggtggt catcaccgcc ccgaaagcac gcacatcgaa gaagttcttg
     1441 cacatccaag aggtcagttc gcgcgccttg gcttcgtcct tgggcagttt ctttgcctct
     1501 gactcgatac cgagcgcctc gtaggcctgt ttgtgctggt tattcagcac ggatttttcc
     1561 tgcatataga tggcatagcc gggggcactt tcctgctcca ggctgacgta gttgcggatc
     1621 ttgcgcttga ggcaaacgtc agtgaccaac ccctggttgg tttccggatc gagacgcggc
     1681 atgttgccag catccgggtc gccattggga ttgccattgc tgacatcaaa gaggtaaacg
     1741 aactcgtagc ggttggagat ggcggtcatt cttctgctcc ttactcgacg gaatcggggc
     1801 cacggttgaa acgggcctgg gcctgttggt agtaaccaat agcaaagcgt ccctggtcct
     1861 ccaggcgcag gcaacgcggg aattggctct gcataccgtc aatgatttcg cctatatcgc
     1921 gctctaggtt cactgctagt ccgggcttct ccttgcgcag cttggccaag tggttttgtg
     1981 tgttgcgcag cagtatcggg aaaaccgtgg ctggcgtgct ggacgcggca ccgtagtagc
     2041 ggtcacggat agtggcattg accctgtcgc ccagggctgc gcgctgggcg ccttccaaca
     2101 cggcgaacag cctccccaag cgatagccgg ggttgctggc gtccttgtcg agactcatag
     2161 gtagctcctc ttggtgaatt ttgccgctca ggcgtgcctc gcgagcgagc acggccttgc
     2221 acagcgcgac gcgtatgcca gagacgtcgc cgtcggcacg catgcgcatg atcaggttgg
     2281 ctagcaaact gcgcggatag cggctgccgg tcaggatggc gcgggtcatt tcaccggcca
     2341 gttgtgggag taagtcttcg gtcttggctc tgccgtcacg actgggcgcg gtagcataga
     2401 gcaagcgcca gatggccggc tccgtcttcc agggcagagg ctcaaggtgc atatcccgga
     2461 aatgctcggc cagatgctgg gtgaataccg caaggctatc gactgcccag aaccgaatgg
     2521 acagtcgcga ggtattgggc gccagcccta acacaaaaat gcgggtgcct tcctccatta
     2581 gcgagtcgag ctcatgcaag ggccgccccg tggccacagc atccagcacg ccgcgcagct
     2641 tttccgcttc ctgaccatca tctgcgggtg gctccagcag gttccagaag gtcgactcgg
     2701 cggcggccac ctgagcagga gtatccgcct gcgcccagaa caccacactc gcgtcgccaa
     2761 tctgcaggcg ctggcggttg tgctcgtcgc gacgcaacaa atggttgagc accgtggtgt
     2821 aggcaaaggc ggcctgttcg gacaccggag cattttcccc ctggctcttg ccgtaggagg
     2881 aaaaagcgtc gaggttgaag gagacgatgg aagcccccga actctgggca ccattgacgc
     2941 ccttgaccgc tggatgcagg cgcgccaacg gctgacgttg gccgcagact aggcacagcc
     3001 cctcgcggct gtcgccgtcg gccaacagcc gggtacgcaa cgccagggcc gccggagtct
     3061 cgtgcagata gcgttgttgg ccgtctaggc ggaacactaa gttgctgtcg agcattgctt
     3121 cgctgaacag cgggggcttg aactgctccg gctgccaaca gtcgaggaac tggagcaagg
     3181 cctgcagacc ggggtcgcct tccccaacca agatctgccg gtgcagcgtc ttgaaggact
     3241 cgtgctcctg ctgagtacgc tctccgccct tggcactaac acccagcaca tagctggtct
     3301 tgtcccacag tacgttggac ttgatgcccg aggtgcgctt ctccggctgc ggcacactca
     3361 taagcctggg ttgaggcttc ttgccagaga gcaagcgaat gtcctgcacg tccagcaact
     3421 ctccttgtgc ggacagcagc agggcgtaac tgatcttttc ttggctgtag ccgaagggtg
     3481 agatattcgc ttcaccccgc tccagcagtc gctgataata gtcattgagg gccgaaagga
     3541 tcatgccttg tcctctgccg ccctgaaggc cggcacctcg atcagcccat cgcgcatcag
     3601 ggcacggaag aagtgcgggg tcatgccatc ggcgaagtca atgtcatgca gcatccagcc
     3661 cagatcacgc tcgccgcgca gaaaggcatc gacttcaggc tcggcactgc cctcttccag
     3721 caaccgaaaa ctggccggaa actcgcgcac gcctaggcag ggtgtatgga agcactgccc
     3781 cttccgtgcg cgacggttga agatatccag atgcttgccc accgtatcgt cggcgccagc
     3841 cctgtcagtc atctcgaaat gcgcctcgat gacataggag acatcgcgca gcagagtagt
     3901 cgcgcgctgc tggcgatcct cctcgaccag attcaccaga ccattagtac gcccggcctt
     3961 cattgcctta ccgacgctga cagcggacag cttgccgccg acctcgttgc gccggatgga
     4021 ttcgaagcgg atcggcttaa gcacttgaat gcgatccacc acccagcgaa tcgccggctt
     4081 ccagtggata gcctcgagaa tgccgcgcgc ggcggacggc gtgatcgcat cgtaagagac
     4141 gcgttccacc ttcatttccg ggcgggtgaa gcaggcacgc tcgccccaga ccattaagcg
     4201 aattccgtag gccatcggcc ctccctttca catcatccag gccaggccgc tgttgcgcgt
     4261 cccgactacc aacatagccg ctcgctgctg acaaaggccg gattatccca gtgcaacccg
     4321 aattggtggt gatacagatc agggttgacc aacgccataa actgctcacc ataacgcgta
     4381 cctgccgccg cctggatcgc accggcttcc cgcaatgcct gataaccctt gcgtggcatc
     4441 tgcaccagat atggctgcag gcgacgggcg atggcggcgc agccgtcggc gaactccagc
     4501 tcgcgcaggg ctgctctggc ctcgtcatca aatgggatga tcaccggcag ttgaaggctg
     4561 tcgatcatgc ggaacttggt ggccaaagtc tcgtagggca ggccatcgag ccggcctctc
     4621 tcaatcaggc cgagcaggtt acccgcatcc aactcctccg cgcccttctg ccagtacagt
     4681 atgcgaaaat accgctcgat ggccgccatg gacaggcaat catccgggtg caggcgcatc
     4741 acttcgcggg cggcctgggc gaactgcttg agttcctcgg gtggcgccca gtcagaattg
     4801 gccgcggcga acaccagcac ctcgctttcg gccagcggcc gcttgccttc gcgattgcag
     4861 cgtcccgcgg cctgggcgat ggaatccaat ccagcctcgg cacgcagtac cacgggaaaa
     4921 tccacatcca caccggcctc gatcagcgag gtggccacca ggcgacaggg ctcccccttt
     4981 ttgagcatct ggcgcacctc ggccagcacg ctgctacggt gcttggcgca catcagggtg
     5041 gtgagatggc gggcaccggg caactcggca agcgactcat agagcgcacg ggcctggcgt
     5101 cgattgttga cgatgcacag cacttgttca cgcctggcga tctgctcagt caaggccgca
     5161 tcttcgagcg ggcccaatgt ccgtattcgt acccgcacca actcccggaa cagccgctgc
     5221 ggctcgggcg ccagctcacg tacgtcctgc agcccaccga tgaaatccgg cgattgaagc
     5281 gctggctgcg tggcagtaca gagaactggg ctacaacggt agttgagcgc cagttcatca
     5341 agggcggcaa cgcagggccg caacagcttg agcggtaggg tctgtgcctc gtcaagaatc
     5401 accacgctgc cggcgatgtt gtgtagcttg cggcactggg ctggacggtc ggcaaacagg
     5461 ctttcgaaga actgcactgc agtggtcacc acgataggcg cgtcccagtt ctccatcgct
     5521 aggttcagtt tcttcttggc ctccaggctc tgccggcggt catcaacgaa ggcgctgtga
     5581 tgctccagca ccgcctcttc gcctaaggcc ccgagtgcac gacggaatac cgcagcgttc
     5641 tgctcgacga tgctagtgaa gggaatcacg tagatcaccc ggcgcagccc atgagctaga
     5701 gcgtgatcca gggcgaaagc cagagaggcc agggtcttgc cgcctcctgt gggcactgtg
     5761 agagaaaaca gcccgggaag ttcgctcgcc ttgccgcgca cgccggccaa tatctccccg
     5821 cgaacgcggt tgaccggcgt atctcccttg aactcagtca gatgccgatc aagggctgcg
     5881 cgtaactcgg ccaaggtcgg ccgcgcttgc tcgcgaaggg agcgccgtcc ttcgacgcgg
     5941 tggtagaagg cttcggtatc tagataatcc gcatcaacca agcaggaaaa gagcatccgg
     6001 ccaagaaagg ccaactgaaa gaaacctctt tccaggcgcg cttttagtgg cggtggttga
     6061 aggcgctcgg gtagcacgat ttccacgcac cacccctcca ataaccgtgg cagcccaacc
     6121 cctttcaggc ggtcgaccaa ggcagttcgc tcaccagcct cgcggccatt ggccaacccg
     6181 gcatggtggc cagcgatgcc gtaggctagc aattgaccta gcgcgccata gcgttctacc
     6241 gccagtatgg ccccgcgagt agagtgatcg acacggatgg catcacccgc aatacgccgc
     6301 tgaaactcgt cagtgtattt acccaagtca tgcaacagac cagcaagagc agctaattca
     6361 ccgccgccga agaaggcagc cttctctcct gccaaacggg caacagcctg tagatgctcg
     6421 accagaggct gccaatcgct gcgatctgcc tttaaggtgg agtgagcaaa aaagtgagta
     6481 tcgctagcct ccgcgtccat aggtgcgctc ccttctcgag gaattcctgc agagcactag
     6541 gtttcaatcc acgcccggtt ttgccgggcg acgagacctt tgtccagaga ggtctcgttt
     6601 caatccacgc gcccgtgcgg ggcgcgacct agttgaattt cattacgacc agtctaaaaa
     6661 gcgcctgaat tcgcgacctt ctcgttactg acaggaaaat gggccattgg caaccaggga
     6721 aagatgaacg tgatgatgtt cacaatttgc tgaattgtgg tgatgtgatg ctcaccgcat
     6781 ttcctgaaaa ttcacgctgt atcttgaaaa atcgacgttt tttacgtggt tttccgtcga
     6841 aaatttaagg taagaacctg acctcgtgat tactatttcg ccgtgttgac gacatcagga
     6901 ggccagtatg accgtattac atagtgtgga tttttttccg tctggtaacg cgtccgtggc
     6961 gatagaaccc cggctcccgc aggcggattt tcctgaacat catcatgatt ttcatgaaat
     7021 tgtgattgtc gaacatggca cgggtattca tgtgtttaat gggcagccct ataccatcac
     7081 cggtggcacg gtctgtttcg tacgcgatca tgatcggcat ctgtatgaac ataccgataa
     7141 tctgtgtctg accaatgtgc tgtatcgctc gccggatcga tttcagtttc tcgccgggct
     7201 gaatcagttg ctgccacaag agctggatgg gcagtatccg tctcactggc gcgttaacca
     7261 cagcgtattg cagcaggtgc gacagctggt tgcacagatg gaacagcagg aaggggaaaa
     7321 tgatttaccc tcgaccgcca gtcgcgagat cttgtttatg caattactgc tcttgctgcg
     7381 taaaagcagt ttgcaggaga acctggaaaa cagcgcatca cgtctcaact tgcttctggc
     7441 ctggctggag gaccattttg ccgatgaggt gaattgggat gccgtggcgg atcaattttc
     7501 tctttcactg cgtacgctac atcggcagct taagcagcaa acgggactga cgcctcagcg
     7561 atacctgaac cgcctgcgac tgatgaaagc ccgacatctg ctacgccaca gcgaggccag
     7621 cgttactgac atcgcctatc gctgtggatt cagcgacagt aaccactttt cgacgctttt
     7681 tcgccgagag tttaactggt caccgcgtga tattcgccag ggacgggatg gctttctgca
     7741 ataacgcgaa tcttctcaac gtatttgtac gccatattgc gaataatcaa cttcgttctc
     7801 tggccgaggt agccacggtg gcgcatcagt taaaacttct caaagatgat ttttttgcca
     7861 gcgaccagca ggcagtcgct gtggctgacc gttatccgca agatgtcttt gctgaacata
     7921 cacatgattt ttgtgagctg gtgattgtct ggcgcggtaa tggcctgcat gtactcaacg
     7981 atcgccctta tcgcattacc cgtggcgatc tcttttacat tcatgctgac gataaacact
     8041 cctacgcttc cgttaacgat ctggttttgc agaatattat ttattgcccg gagcgtctga
     8101 agctgaatct tgactggcag ggggcgattc cgggatttaa cgccagcgca gggcaaccac
     8161 actggcgctt aggtagcatg gggatggcgc aggcgcggca ggttatcggt cagcttgagc
     8221 atgaaagtag tcagcatgtg ccgtttgcta acgaaatggc tgagttgctg ttcgggcagt
     8281 tggtgatgtt gctgaatcgc catcgttaca ccagtgattc gttgccgcca acatccagcg
     8341 aaacgttgct ggataagctg attacccggc tggcggctag cctgaaaagt ccctttgcgc
     8401 tggataaatt ttgtgatgag gcatcgtgca gtgagcgcgt tttgcgtcag caatttcgcc
     8461 agcagactgg aatgaccatc aatcaatatc tgcgacaggt cagagtgtgt catgcgcaat
     8521 atcttctcca gcatagccgc ctgttaatca gtgatatttc gaccgaatgt ggctttgaag
     8581 atagtaacta tttttcggtg gtgtttaccc gggaaaccgg gatgacgccc agccagtggc
     8641 gtcatctcaa ttcgcagaaa gattaactag gccagatcca gcggcatctg ggttagtcga
     8701 gcgcgggccg cttcccatgt ctcaccaggg cgagcctgtt tcgcgatctc agcatctgaa
     8761 atcttcccgg ccttgcgcct cgctggggcc ttacccaccg ccttggcggg cttcttcggt
     8821 ccaaaactga acaacagatg tgtgaccttg cgcccggtct ttcgctgcgc ccactccacc
     8881 tgtagcgggc tgtgctcgtt gatctgcgtc acggctggat caagcactcg caacttgaag
     8941 tccttgatcg agggataccg gccttccagt tgaaaccact ttcgcagctg gtcaatttct
     9001 atttcgcgct ggccgatgct gtcccattgc atgagcagct cgtaaagcct gatcgcgtgg
     9061 gtgctgtcca tcttggccac gtcagccaag gcgtatttgg tgaactgttt ggtgagttcc
     9121 gtcaggtacg gcagcatgtc tttggtgaac ctgagttcta cacggccctc accctcccgg
     9181 tagatgattg tttgcaccca gccggtaatc atcacactcg gtcttttccc cttgccattg
     9241 ggctcttggg ttaaccggac ttcccgccgt ttcaggcgca gggccgcttc tttgagctgg
     9301 ttgtaggaag attcgatagg gacacccgcc atcgtcgcta tgtcctccgc cgtcactgaa
     9361 tacatcactt catcggtgac aggctcgctc ctcttcacct ggctaataca ggccagaacg
     9421 atccgctgtt cctgaacact gaggcgatac gcggcctcga ccagggcatt gcttttgtaa
     9481 accattgggg gtgaggccac gttcgacatt ccttgtgtat aaggggacac tgtatctgcg
     9541 tcccacaata caacaaatcc gtccctttac aacaacaaat ccgtcccttc ttaacaacaa
     9601 atccgtccct taatggcaac aaatccgtcc ctttttaaac tctacaggcc acggattacg
     9661 tggcctgtag acgtcctaaa aggtttaaaa gggaaaagga agaaaagggt ggaaacgcaa
     9721 aaaacgcacc actacgtggc cccgttgggg ccgcatttgt gcccctgaag gggcggggga
     9781 ggcgtctggg caatccccgt tttaccagtc ccctatcgcc gcctgagagg gcgcaggaag
     9841 cgagtaatca gggtatcgag gcggattcac ccttggcgtc caaccagcgg caccagcggc
     9901 gcgaattgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt atgcgctcac
     9961 gcaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
    10021 aaaggacaag ggtcgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt
    10081 accaatgttt aatcaggctc gcgccaattt ccgcaatctg acggttacgt tcatccatcg
    10141 tcgcctggct gccggtggta taaatcacca caatacggct cggtttacca tccgggccca
    10201 gcgccgcaat aatgccacgg ctgccacgtt cgcccgcacc gcttttatcc gcaataaacc
    10261 agccagccgg cagcgcgcta cgcagcagcg gacccgccac tttatccgct tccatccaat
    10321 caatcagttg ctggcggctt gccagggtca gcagctcacc cgtcagcagt ttacgcaggg
    10381 tggtcgccat tgctgccggc atggtggtat cacgttcatc gttcggaatc gcttcgttca
    10441 gttccggttc ccaacgatcc agacgggtca catgatcgcc catgttatgc agaaacgcgg
    10501 tcagttcttt cggaccgcca atggtcgtca gcagcaggtt cgccgcggtg ttatcgctca
    10561 tggtaatcgc cgcgctgcac agttcacgca cggtcatgcc atcggtcaga tgtttttcgg
    10621 tcaccgggct atattccacc agatcgttct ggctataatg aatacgacgg cccagttgtt
    10681 cctggcccgc atccacacgg ctcagaaccg caccgcacag cagcacttta aaggtgctca
    10741 tcatcggaaa acgttcttcc ggacgaaaag attccagaat tttgccgctg ttcagatcca
    10801 gttcaatata gcccacgcgc gcacccagtt gatcttccgc atctttcact ttcaccaggg
    10861 tttccggatg cgcaaacacc ggcaggcaaa acgccgcaaa aaacggaatc agcgccacac
    10921 gaaaatgctg aatgctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggaat
    11041 taaaaaaaag cccgctcatt aggcgggcta ctaatttaaa tcgtaattat tggggacccc
    11101 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
    11161 gagttctgag gtcattactg gatctatcaa caggagtagc gtcagacccc gtagaaaaga
    11221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaa
//
