LOCUS       sequence-416786-       11235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses all necessary components of Type I-C CRISPR-Cas system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Lammens E-M, Volke DC, Schroven K, Voet M, Kerremans A, Lavigne R, 
            Hendrix H
  TITLE     A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for 
            Pseudomonas with efficient vector curing.
  JOURNAL   Microbiol Spectr. 2023 Nov 17:e0270723. doi: 
            10.1128/spectrum.02707-23.
  PUBMED    37975669
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23."
COMMENT     Sequence Label: pCas3-Sm
FEATURES             Location/Qualifiers
     source          1..11235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             158..949
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(join(1050..1055,1056..1072,1073..1078))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      1119..1470
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1484..2317
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPARRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(2318..3166)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(3240..4076)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        4222..4340
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     regulatory      6273..6282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7449..7460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     oriT            10261..10369
                     /note="incP origin of transfer"
     rep_origin      complement(10439..11027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10519..10538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      11064..11157
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
       61 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      121 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      181 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      241 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      301 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      361 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      421 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      481 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      541 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      601 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      661 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      721 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      781 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      841 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
      901 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcc
      961 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     1021 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaatt
     1081 cgcgccgctg gtgccgctgg ttggacgcca agggtgaatc cgcctcgata ccctgattac
     1141 tcgcttcctg cgccctctca ggcggcgata ggggactggt aaaacgggga ttgcccagac
     1201 gcctcccccg ccccttcagg ggcacaaatg cggccccaac ggggccacgt agtggtgcgt
     1261 tttttgcgtt tccacccttt tcttcctttt cccttttaaa ccttttagga cgtctacagg
     1321 ccacgtaatc cgtggcctgt agagtttaaa aagggacgga tttgttgcca ttaagggacg
     1381 gatttgttgt taagaaggga cggatttgtt gttgtaaagg gacggatttg ttgtattgtg
     1441 ggacgcagat acagtgtccc cttatacaca aggaatgtcg aacgtggcct cacccccaat
     1501 ggtttacaaa agcaatgccc tggtcgaggc cgcgtatcgc ctcagtgttc aggaacagcg
     1561 gatcgttctg gcctgtatta gccaggtgaa gaggagcgag cctgtcaccg atgaagtgat
     1621 gtattcagtg acggcggagg acatagcgac gatggcgggt gtccctatcg aatcttccta
     1681 caaccagctc aaagaagcgg ccctgcgcct gaaacggcgg gaagtccggt taacccaaga
     1741 gcccaatggc aaggggaaaa gaccgagtgt gatgattacc ggctgggtgc aaacaatcat
     1801 ctaccgggag ggtgagggcc gtgtagaact caggttcacc aaagacatgc tgccgtacct
     1861 gacggaactc accaaacagt tcaccaaata cgccttggct gacgtggcca agatggacag
     1921 cacccacgcg atcaggcttt acgagctgct catgcaatgg gacagcatcg gccagcgcga
     1981 aatagaaatt gaccagctgc gaaagtggtt tcaactggaa ggccggtatc cctcgatcaa
     2041 ggacttcaag ttgcgagtgc ttgatccagc cgtgacgcag atcaacgagc acagcccgct
     2101 acaggtggag tgggcgcagc gaaagaccgg gcgcaaggtc acacatctgt tgttcagttt
     2161 tggaccgaag aagcccgcca aggcggtggg taaggcccca gcgaggcgca aggccgggaa
     2221 gatttcagat gctgagatcg cgaaacaggc tcgccctggt gagacatggg aagcggcccg
     2281 cgctcgacta acccagatgc cgctggatct ggcctagtta atctttctgc gaattgagat
     2341 gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa aaatagttac
     2401 tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta tgctggagaa
     2461 gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc attccagtct
     2521 gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca caaaatttat
     2581 ccagcgcaaa gggacttttc aggctagccg ccagccgggt aatcagctta tccagcaacg
     2641 tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc agcaacatca
     2701 ccaactgccc gaacagcaac tcagccattt cgttagcaaa cggcacatgc tgactacttt
     2761 catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccatgcta cctaagcgcc
     2821 agtgtggttg ccctgcgctg gcgttaaatc ccggaatcgc cccctgccag tcaagattca
     2881 gcttcagacg ctccgggcaa taaataatat tctgcaaaac cagatcgtta acggaagcgt
     2941 aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc acgggtaatg cgataagggc
     3001 gatcgttgag tacatgcagg ccattaccgc gccagacaat caccagctca caaaaatcat
     3061 gtgtatgttc agcaaagaca tcttgcggat aacggtcagc cacagcgact gcctgctggt
     3121 cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg gctacctcgg
     3181 ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga agattcgcgt
     3241 tattgcagaa agccatcccg tccctggcga atatcacgcg gtgaccagtt aaactctcgg
     3301 cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agcgataggc gatgtcagta
     3361 acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag gcggttcagg
     3421 tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt acgcagtgaa
     3481 agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg gtcctccagc
     3541 caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg caaactgctt
     3601 ttacgcagca agagcagtaa ttgcataaac aagatctcgc gactggcggt cgagggtaaa
     3661 tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg ctgcaatacg
     3721 ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg cagcaactga
     3781 ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt ggtcagacac
     3841 agattatcgg tatgttcata cagatgccga tcatgatcgc gtacgaaaca gaccgtgcca
     3901 ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg ttcgacaatc
     3961 acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag ccggggttct
     4021 atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac ggtcatactg
     4081 gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc ttaccttaaa
     4141 ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt gaattttcag
     4201 gaaatgcggt gagcatcaca tcaccacaat tcagcaaatt gtgaacatca tcacgttcat
     4261 ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtcg cgaattcagg
     4321 cgctttttag actggtcgta atgaaattca actaggtcgc gccccgcacg ggcgcgtgga
     4381 ttgaaacgag acctctctgg acaaaggtct cgtcgcccgg caaaaccggg cgtggattga
     4441 aacctagtgc tctgcaggaa ttcctcgaga agggagcgca cctatggacg cggaggctag
     4501 cgatactcac ttttttgctc actccacctt aaaggcagat cgcagcgatt ggcagcctct
     4561 ggtcgagcat ctacaggctg ttgcccgttt ggcaggagag aaggctgcct tcttcggcgg
     4621 cggtgaatta gctgctcttg ctggtctgtt gcatgacttg ggtaaataca ctgacgagtt
     4681 tcagcggcgt attgcgggtg atgccatccg tgtcgatcac tctactcgcg gggccatact
     4741 ggcggtagaa cgctatggcg cgctaggtca attgctagcc tacggcatcg ctggccacca
     4801 tgccgggttg gccaatggcc gcgaggctgg tgagcgaact gccttggtcg accgcctgaa
     4861 aggggttggg ctgccacggt tattggaggg gtggtgcgtg gaaatcgtgc tacccgagcg
     4921 ccttcaacca ccgccactaa aagcgcgcct ggaaagaggt ttctttcagt tggcctttct
     4981 tggccggatg ctcttttcct gcttggttga tgcggattat ctagataccg aagccttcta
     5041 ccaccgcgtc gaaggacggc gctcccttcg cgagcaagcg cggccgacct tggccgagtt
     5101 acgcgcagcc cttgatcggc atctgactga gttcaaggga gatacgccgg tcaaccgcgt
     5161 tcgcggggag atattggccg gcgtgcgcgg caaggcgagc gaacttcccg ggctgttttc
     5221 tctcacagtg cccacaggag gcggcaagac cctggcctct ctggctttcg ccctggatca
     5281 cgctctagct catgggctgc gccgggtgat ctacgtgatt cccttcacta gcatcgtcga
     5341 gcagaacgct gcggtattcc gtcgtgcact cggggcctta ggcgaagagg cggtgctgga
     5401 gcatcacagc gccttcgttg atgaccgccg gcagagcctg gaggccaaga agaaactgaa
     5461 cctagcgatg gagaactggg acgcgcctat cgtggtgacc actgcagtgc agttcttcga
     5521 aagcctgttt gccgaccgtc cagcccagtg ccgcaagcta cacaacatcg ccggcagcgt
     5581 ggtgattctt gacgaggcac agaccctacc gctcaagctg ttgcggccct gcgttgccgc
     5641 ccttgatgaa ctggcgctca actaccgttg tagcccagtt ctctgtactg ccacgcagcc
     5701 agcgcttcaa tcgccggatt tcatcggtgg gctgcaggac gtacgtgagc tggcgcccga
     5761 gccgcagcgg ctgttccggg agttggtgcg ggtacgaata cggacattgg gcccgctcga
     5821 agatgcggcc ttgactgagc agatcgccag gcgtgaacaa gtgctgtgca tcgtcaacaa
     5881 tcgacgccag gcccgtgcgc tctatgagtc gcttgccgag ttgcccggtg cccgccatct
     5941 caccaccctg atgtgcgcca agcaccgtag cagcgtgctg gccgaggtgc gccagatgct
     6001 caaaaagggg gagccctgtc gcctggtggc cacctcgctg atcgaggccg gtgtggatgt
     6061 ggattttccc gtggtactgc gtgccgaggc tggattggat tccatcgccc aggccgcggg
     6121 acgctgcaat cgcgaaggca agcggccgct ggccgaaagc gaggtgctgg tgttcgccgc
     6181 ggccaattct gactgggcgc cacccgagga actcaagcag ttcgcccagg ccgcccgcga
     6241 agtgatgcgc ctgcacccgg atgattgcct gtccatggcg gccatcgagc ggtattttcg
     6301 catactgtac tggcagaagg gcgcggagga gttggatgcg ggtaacctgc tcggcctgat
     6361 tgagagaggc cggctcgatg gcctgcccta cgagactttg gccaccaagt tccgcatgat
     6421 cgacagcctt caactgccgg tgatcatccc atttgatgac gaggccagag cagccctgcg
     6481 cgagctggag ttcgccgacg gctgcgccgc catcgcccgt cgcctgcagc catatctggt
     6541 gcagatgcca cgcaagggtt atcaggcatt gcgggaagcc ggtgcgatcc aggcggcggc
     6601 aggtacgcgt tatggtgagc agtttatggc gttggtcaac cctgatctgt atcaccacca
     6661 attcgggttg cactgggata atccggcctt tgtcagcagc gagcggctat gttggtagtc
     6721 gggacgcgca acagcggcct ggcctggatg atgtgaaagg gagggccgat ggcctacgga
     6781 attcgcttaa tggtctgggg cgagcgtgcc tgcttcaccc gcccggaaat gaaggtggaa
     6841 cgcgtctctt acgatgcgat cacgccgtcc gccgcgcgcg gcattctcga ggctatccac
     6901 tggaagccgg cgattcgctg ggtggtggat cgcattcaag tgcttaagcc gatccgcttc
     6961 gaatccatcc ggcgcaacga ggtcggcggc aagctgtccg ctgtcagcgt cggtaaggca
     7021 atgaaggccg ggcgtactaa tggtctggtg aatctggtcg aggaggatcg ccagcagcgc
     7081 gcgactactc tgctgcgcga tgtctcctat gtcatcgagg cgcatttcga gatgactgac
     7141 agggctggcg ccgacgatac ggtgggcaag catctggata tcttcaaccg tcgcgcacgg
     7201 aaggggcagt gcttccatac accctgccta ggcgtgcgcg agtttccggc cagttttcgg
     7261 ttgctggaag agggcagtgc cgagcctgaa gtcgatgcct ttctgcgcgg cgagcgtgat
     7321 ctgggctgga tgctgcatga cattgacttc gccgatggca tgaccccgca cttcttccgt
     7381 gccctgatgc gcgatgggct gatcgaggtg ccggccttca gggcggcaga ggacaaggca
     7441 tgatcctttc ggccctcaat gactattatc agcgactgct ggagcggggt gaagcgaata
     7501 tctcaccctt cggctacagc caagaaaaga tcagttacgc cctgctgctg tccgcacaag
     7561 gagagttgct ggacgtgcag gacattcgct tgctctctgg caagaagcct caacccaggc
     7621 ttatgagtgt gccgcagccg gagaagcgca cctcgggcat caagtccaac gtactgtggg
     7681 acaagaccag ctatgtgctg ggtgttagtg ccaagggcgg agagcgtact cagcaggagc
     7741 acgagtcctt caagacgctg caccggcaga tcttggttgg ggaaggcgac cccggtctgc
     7801 aggccttgct ccagttcctc gactgttggc agccggagca gttcaagccc ccgctgttca
     7861 gcgaagcaat gctcgacagc aacttagtgt tccgcctaga cggccaacaa cgctatctgc
     7921 acgagactcc ggcggccctg gcgttgcgta cccggctgtt ggccgacggc gacagccgcg
     7981 aggggctgtg cctagtctgc ggccaacgtc agccgttggc gcgcctgcat ccagcggtca
     8041 agggcgtcaa tggtgcccag agttcggggg cttccatcgt ctccttcaac ctcgacgctt
     8101 tttcctccta cggcaagagc cagggggaaa atgctccggt gtccgaacag gccgcctttg
     8161 cctacaccac ggtgctcaac catttgttgc gtcgcgacga gcacaaccgc cagcgcctgc
     8221 agattggcga cgcgagtgtg gtgttctggg cgcaggcgga tactcctgct caggtggccg
     8281 ccgccgagtc gaccttctgg aacctgctgg agccacccgc agatgatggt caggaagcgg
     8341 aaaagctgcg cggcgtgctg gatgctgtgg ccacggggcg gcccttgcat gagctcgact
     8401 cgctaatgga ggaaggcacc cgcatttttg tgttagggct ggcgcccaat acctcgcgac
     8461 tgtccattcg gttctgggca gtcgatagcc ttgcggtatt cacccagcat ctggccgagc
     8521 atttccggga tatgcacctt gagcctctgc cctggaagac ggagccggcc atctggcgct
     8581 tgctctatgc taccgcgccc agtcgtgacg gcagagccaa gaccgaagac ttactcccac
     8641 aactggccgg tgaaatgacc cgcgccatcc tgaccggcag ccgctatccg cgcagtttgc
     8701 tagccaacct gatcatgcgc atgcgtgccg acggcgacgt ctctggcata cgcgtcgcgc
     8761 tgtgcaaggc cgtgctcgct cgcgaggcac gcctgagcgg caaaattcac caagaggagc
     8821 tacctatgag tctcgacaag gacgccagca accccggcta tcgcttgggg aggctgttcg
     8881 ccgtgttgga aggcgcccag cgcgcagccc tgggcgacag ggtcaatgcc actatccgtg
     8941 accgctacta cggtgccgcg tccagcacgc cagccacggt tttcccgata ctgctgcgca
     9001 acacacaaaa ccacttggcc aagctgcgca aggagaagcc cggactagca gtgaacctag
     9061 agcgcgatat aggcgaaatc attgacggta tgcagagcca attcccgcgt tgcctgcgcc
     9121 tggaggacca gggacgcttt gctattggtt actaccaaca ggcccaggcc cgtttcaacc
     9181 gtggccccga ttccgtcgag taaggagcag aagaatgacc gccatctcca accgctacga
     9241 gttcgtttac ctctttgatg tcagcaatgg caatcccaat ggcgacccgg atgctggcaa
     9301 catgccgcgt ctcgatccgg aaaccaacca ggggttggtc actgacgttt gcctcaagcg
     9361 caagatccgc aactacgtca gcctggagca ggaaagtgcc cccggctatg ccatctatat
     9421 gcaggaaaaa tccgtgctga ataaccagca caaacaggcc tacgaggcgc tcggtatcga
     9481 gtcagaggca aagaaactgc ccaaggacga agccaaggcg cgcgaactga cctcttggat
     9541 gtgcaagaac ttcttcgatg tgcgtgcttt cggggcggtg atgaccaccg agattaatgc
     9601 cggccaggtg cgtggaccga tccaactggc attcgccacg tctatcgacc cggtattgcc
     9661 tatggaggta tccatcaccc gcatggcggt gactaacgaa aaggatttgg agaaggaacg
     9721 caccatggga cgcaagcaca tcgtgcctta cggcttgtac cgcgcccatg gtttcatctc
     9781 tgccaagttg gccgagcgaa ccggcttttc cgacgacgac ttggaactgc tatggcgcgc
     9841 tttggccaat atgttcgaac acgaccgctc ggcggcacgt ggcgagatgg cagcgcgcaa
     9901 gttgatcgtc ttcaagcatg agcatgccat gggcaatgca cccgcccatg tgctgttcgg
     9961 cagcgttaag gtcgagcgag tcgaggggga cgcagttaca ccagcacgcg gtttccagga
    10021 ttaccgtgtc agcatcgatg cggaagctct gcctcagggc gtgagcgtgc gcgagtacct
    10081 ctagtttcag gtatcgatac cgtcgacggg gattccttaa ggtatacttt ccgctgcata
    10141 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
    10201 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
    10261 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
    10321 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccga taagctagct
    10381 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10501 gaaacccgac aggactataa agataccagg cgtttccccc tggaggctcc ctcgtgcgct
    10561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10861 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11041 tcttttctac ggggtctgac gctactcctg ttgatagatc cagtaatgac ctcagaactc
    11101 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
    11161 ggggtcccca ataattacga tttacgtatt taaatgaacc ttgaccgaac gcagcggtgg
    11221 taacggcgca gtggc
//
