LOCUS       sequence-41679-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-4SA(S164)
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..62
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattgat ctaccatgga ctacaaagac gatgacgaca
       61 agcttgcggc cgcgaattca agcttagcca ccatggacta caaagacgat gacgataaag
      121 caaggctcga atcggtacct aaggatcccg ggcagcagcc gccgcctcaa ccggcccccc
      181 agggccaagg gcagccgcct tcgcagcccc cgcaggggca gggcccgccg tccggacccg
      241 ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc accccccgcc gggcatcaga
      301 tcgtgcacgt ccgcggggac gcggagaccg acctggaggc gctcttcaac gccgtcatga
      361 accccaagac ggccaacgtg ccccagaccg tgcccatgag gctccggaag ctgcccgact
      421 ccttcttcaa gccgccggag cccaaatccc actcccgaca ggccgctact gatgcaggca
      481 ctgcaggagc cctgactcca cagcatgttc gagctcatgc cgctccagct gctctgcagt
      541 tgggagctgt ttctcctggg acactgaccc ccactggagt agtctctggc ccagcagcta
      601 cacccacagc tcagcatctt cgacagtctt cttttgagat acctgatgat gtacctctgc
      661 cagcaggttg ggagatggca aagacatctt ctggtcagag atacttctta aatcacatcg
      721 atcagacaac aacatggcag gaccccagga aggccatgct gtcccagatg aacgtcacag
      781 cccccaccag tccaccagtg cagcagaata tgatgaactc ggcttcaggt cctcttcctg
      841 atggatggga acaagccatg actc
//
