LOCUS       sequence-416791-        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pUB_SM-RLuc0---MS2
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3213..3236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3243..3266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3309..3332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3552..3575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3639..4571
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="TSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    6310..6898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6363..6383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6781..6792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6973..7206
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7325..7343)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7411..7515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7516..7584,7585..8376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7734..7753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8547..9135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9036..9055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9289..9306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9546..9567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9582..9587,9588..9605,9606..9612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9620..9636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9625..9647
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9644..9660
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9644..9660
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9710..9729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
     2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
     2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
     2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
     2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
     2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
     2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
     3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
     3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
     3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
     3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
     3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
     3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
     3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
     3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
     3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
     3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
     3601 agggaggcgg tggatcaggt ggaggaggtt cactgcagac ttcgaaagtt tatgatccag
     3661 aacaaaggaa acggatgata actggtccgc agtggtgggc cagatgtaaa caaatgaatg
     3721 ttcttgattc atttattaat tattatgatt cagaaaaaca tgcagaaaat gctgttattt
     3781 ttttacatgg taacgcggcc tcttcttatt tatggcgaca tgttgtgcca catattgagc
     3841 cagtagcgcg gtgtattata ccagacctta ttggtatggg caaatcaggc aaatctggta
     3901 atggttctta taggttactt gatcattaca aatatcttac tgcatggttt gaacttctta
     3961 atttaccaaa gaagatcatt tttgtcggcc atgattgggg tgcttgtttg gcatttcatt
     4021 atagctatga gcatcaagat aagatcaaag caatagttca cgctgaaagt gtagtagatg
     4081 tgattgaatc atgggatgaa tggcctgata ttgaagaaga tattgcgttg atcaaatctg
     4141 aagaaggaga aaaaatggtt ttggagaata acttcttcgt ggaaaccatg ttgccatcaa
     4201 aaatcatgag aaagttagaa ccagaagaat ttgcagcata tcttgaacca ttcaaagaga
     4261 aaggtgaagt tcgtcgtcca acattatcat ggcctcgtga aatcccgtta gtaaaaggtg
     4321 gtaaacctga cgttgtacaa attgttagga attataatgc ttatctacgt gcaagtgatg
     4381 atttaccaaa aatgtttatt gaatcggacc caggattctt ttccaatgct attgttgaag
     4441 gtgccaagaa gtttcctaat actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag
     4501 aagatgcacc tgatgaaatg ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa
     4561 atgaacaata agctagctag gcggactgtt actgagctgc gttttacacc ctttctttga
     4621 caaaacctaa cttgcgcaga aaaaaaaaaa ataagagaca acattggcat ggctttgttt
     4681 ttttaaattt tttttaaagt tttttttttt tttttttttt tttaagtttt tttgttttgt
     4741 tttggcgctt ttgactcagg atttaaaaac tggaacggtg aaggcgacag cagttggttg
     4801 gagcaaacat cccccaaagt tctacaaatg tggctgagga ctttgtacat tgttttgttt
     4861 tttttttttt tggttttgtc tttttttaat agtcattcca agtatccatg aaataagtgg
     4921 ttacaggaag tccctcaccc tcccaaaagc cacccccact cctaagagga ggatggtcgc
     4981 gtccatgccc tgagtccacc ccggggaagg tgacaaccgg taacctacaa acgggtggag
     5041 gatcacccca cccgacactt cacaatcaag gggtacaata cacaagggtg gaggaacacc
     5101 ccaccctcca gacacattac acagaaatcc aatcaaacag aagcaccatc agggcttctg
     5161 ctaccaaatt tatctcaaaa aactacaaca aggaatcacc atcagggatt ccctgtgcaa
     5221 tatacgtcaa acgagggcca cgacgggagg acgatcacgc ctcccgaata tcggcatgtc
     5281 tggctttcga attcagtgcg tggagcatca gcccacgcag ccaatcagag tcgaatacaa
     5341 gtcgactttc gcgaagagca tcagccttcg cgccattctt acacaaacca cactctcccc
     5401 tacaggaaca gcatcagcgt tcctgcccag tacccaactc aagaaaattt atgtccccat
     5461 gcagcatcag cgcatgggcc ccaagaatac atccccaaca aaatcacatc cgagcaccaa
     5521 cagggctcgg agtgttgttt cttgtccaac tggacaaacc ctccatggac catcaggcca
     5581 tggactctca ccaacaagac aaaaactact cttctcgaag cagcatcagc gcttcgaaac
     5641 actcgagcat acattgtgcc tatttcttgg gtggacgatc acgccaccca tgctctcacg
     5701 aatttcaaaa cacggacaag gacgagcacc accagggctc gtcgttccac gtccaatacg
     5761 attacttacc tttcgggatc acgatcacgg atcccgcagc tacatcactt ccactcagga
     5821 cattcaagca tgcacgatca cggcatgctc cacaagtctc aaccacagaa actaccaaat
     5881 gggttcagca ccagcgaacc cactcctacc tcaaacctct tcccacaaaa ctggcaagca
     5941 ggatcaccgc ttgcccattc caacatacca aatcaaaaac aattactggt acagcatcag
     6001 cgtaccagcc cacatctctc actactatca aaaaccaaac cgttcagcaa cagcgaacgg
     6061 tacacacgga aaaatcaact ggtttacaaa tacgaaagac gatcacgctt tcgtccagcg
     6121 caaactatta cgaaaaacat ccgacgggaa gagcaacagc cttcccgcgg cggaaaacct
     6181 cacaaaaaca cgacaaacgg atgcacgaac acggcatccg ccgacaaccc acaaacttac
     6241 aaccaggcaa acggtgcagg atcaccgcac cgtacatcaa acacctcaga tctcatatcg
     6301 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6841 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6901 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6961 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7021 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7081 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7141 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7201 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7261 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     7321 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7381 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7741 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     7801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8041 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9301 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9361 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9421 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9481 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9541 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9601 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9661 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9721 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9781 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9841 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9901 tgtactacaa acttagtagt
//
