LOCUS       sequence-416803-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SM(FLAG)-optimal Firefly luciferase-optimal GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pUB_SM-Fluc100-GFPOPT
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3213..3236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3243..3266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3309..3332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3552..3575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7758..8346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7811..7831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8229..8240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8421..8654
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8773..8791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8859..8963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8964..9032,9033..9824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9182..9201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9995..10583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10484..10503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10737..10754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10994..11015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11030..11035,11036..11053,11054..11060)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11068..11084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11073..11095
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11092..11108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11092..11108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11158..11177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
     2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
     2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
     2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
     2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
     2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
     2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
     3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
     3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
     3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
     3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
     3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
     3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
     3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
     3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
     3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
     3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
     3601 agggaggcgg tggatcaggt ggaggaggtt cactgcagga ggacgccaaa aacattaaaa
     3661 aaggcccggc tccattctac ccgctggagg acggtactgc tggcgagcag ctgcacaaag
     3721 ctatgaaacg atacgcacta gttccgggta ctattgcttt cactgacgct cacatcgagg
     3781 tggacatcac ttacgctgag tacttcgaga tgagcgttcg gttggccgag gctatgaaac
     3841 gatacggcct gaacactaac caccgaatcg tggtgtgctc agagaactca ttacagttct
     3901 tcatgccggt gttgggcgcc ttattcatcg gcgttgctgt tgccccagcc aacgacattt
     3961 acaacgagcg agagttgtta aactcaatgg gcatttcaca gccaactgtg gtgttcgttt
     4021 caaaaaaagg cttgcagaaa attttgaacg tgcagaaaaa attaccaatc atccagaaaa
     4081 ttattatcat ggacagcaaa actgactacc agggtttcca gagcatgtac actttcgtta
     4141 cttcacactt gccaccgggt ttcaacgagt acgacttcgt gccagagagc ttcgaccgag
     4201 acaaaactat tgccctgatc atgaactcat caggctcaac tggtctgccg aaaggtgttg
     4261 ctctgccgca ccggactgcc tgcgtgcgat tctcacacgc ccgggaccca attttcggca
     4321 accagatcat tccggacact gccattttaa gcgttgttcc attccaccac ggtttcggca
     4381 tgttcactac tctgggttac ttcatatgcg gtttccgagt tgtgttaatg taccggttcg
     4441 aggaggagct gttcctgcga agcttgcagg actacaaaat tcagtcagct ctgctggtgc
     4501 caactctgtt cagcttcttc gccaaaagca ctctgattga caaatacgac ttatcaaact
     4561 tacacgagat tgcttcaggt ggcgctccgt tatcaaaaga ggttggcgag gccgttgcca
     4621 aacgattcca cctgccaggt atccgacagg gttacggttt gactgagact acttcagcta
     4681 ttctgattac tccggagggt gacgacaaac cgggcgctgt gggtaaagtt gttccattct
     4741 tcgaggctaa agttgtggac ctggacactg gtaaaactct gggcgttaac cagcgaggcg
     4801 agctgtgcgt gcgaggtccg atgattatga gcggttacgt gaacaacccg gaggctacta
     4861 acgccttgat tgacaaagac ggttggttac acagcggtga catcgcttac tgggacgagg
     4921 acgagcactt cttcatcgtt gaccggctga aatcactgat taaatacaaa ggctaccagg
     4981 tggctccggc tgagttggag tcaatcttgt tacagcaccc aaacatcttc gacgctggtg
     5041 tggctggtct gccggacgac gacgccggtg agttaccagc cgccgttgtt gttttggagc
     5101 acggtaaaac tatgactgag aaagagatcg tggactacgt tgccagccag gttactactg
     5161 ctaaaaaatt gcggggtggc gttgtgttcg tcgacgaggt tccgaaaggt ttaactggca
     5221 aattggacgc tcgaaaaatc cgagagatcc tgatcaaagc caaaaaaggc ggcaaaatcg
     5281 ccgtgggtac ctatacgcgt gtgagtaaag gcgaggagct gttcactggc gtggttccaa
     5341 ttctggttga gctggacggt gacgtgaacg gtcacaaatt ctcagttagc ggcgagggtg
     5401 agggcgacgc tacctacggt aaactgacct tgaaattcat ttgcactact ggtaaattac
     5461 cagttccatg gccaacttta gttactactt tgacctacgg tgttcagtgc ttcagtcggt
     5521 acccggacca catgaaacag cacgacttct tcaaatcagc tatgccagag ggctacgtgc
     5581 aggagcgtac cattttcttc aaagacgacg gtaactacaa aacccgagct gaggttaaat
     5641 tcgagggcga caccttggtt aaccgtattg agttgaaagg tattgacttc aaagaggacg
     5701 gcaacatttt gggccacaaa ttagagtaca actacaacag ccacaacgtt tacattatgg
     5761 ctgacaaaca gaaaaacggc attaaagtga acttcaaaat tcggcacaac attgaggacg
     5821 gcagcgttca gctggccgac cactaccagc agaacacccc aattggtgac ggtccagtgc
     5881 tgttgccgga caaccactac ctgagcactc agagcgcctt aagtaaagac ccaaacgaga
     5941 aacgtgacca catggtgtta ttggagttcg tgactgctgc tggcattacc ctgggcatgg
     6001 acgagtaaat gcatcagccg ctagctaggc ggactgttac tgagctgcgt tttacaccct
     6061 ttctttgaca aaacctaact tgcgcagaaa aaaaaaaaat aagagacaac attggcatgg
     6121 ctttgttttt ttaaattttt tttaaagttt tttttttttt tttttttttt taagtttttt
     6181 tgttttgttt tggcgctttt gactcaggat ttaaaaactg gaacggtgaa ggcgacagca
     6241 gttggttgga gcaaacatcc cccaaagttc tacaaatgtg gctgaggact ttgtacattg
     6301 ttttgttttt tttttttttg gttttgtctt tttttaatag tcattccaag tatccatgaa
     6361 ataagtggtt acaggaagtc cctcaccctc ccaaaagcca cccccactcc taagaggagg
     6421 atggtcgcgt ccatgccctg agtccacccc ggggaaggtg acaaccggta acctacaaac
     6481 gggtggagga tcaccccacc cgacacttca caatcaaggg gtacaataca caagggtgga
     6541 ggaacacccc accctccaga cacattacac agaaatccaa tcaaacagaa gcaccatcag
     6601 ggcttctgct accaaattta tctcaaaaaa ctacaacaag gaatcaccat cagggattcc
     6661 ctgtgcaata tacgtcaaac gagggccacg acgggaggac gatcacgcct cccgaatatc
     6721 ggcatgtctg gctttcgaat tcagtgcgtg gagcatcagc ccacgcagcc aatcagagtc
     6781 gaatacaagt cgactttcgc gaagagcatc agccttcgcg ccattcttac acaaaccaca
     6841 ctctccccta caggaacagc atcagcgttc ctgcccagta cccaactcaa gaaaatttat
     6901 gtccccatgc agcatcagcg catgggcccc aagaatacat ccccaacaaa atcacatccg
     6961 agcaccaaca gggctcggag tgttgtttct tgtccaactg gacaaaccct ccatggacca
     7021 tcaggccatg gactctcacc aacaagacaa aaactactct tctcgaagca gcatcagcgc
     7081 ttcgaaacac tcgagcatac attgtgccta tttcttgggt ggacgatcac gccacccatg
     7141 ctctcacgaa tttcaaaaca cggacaagga cgagcaccac cagggctcgt cgttccacgt
     7201 ccaatacgat tacttacctt tcgggatcac gatcacggat cccgcagcta catcacttcc
     7261 actcaggaca ttcaagcatg cacgatcacg gcatgctcca caagtctcaa ccacagaaac
     7321 taccaaatgg gttcagcacc agcgaaccca ctcctacctc aaacctcttc ccacaaaact
     7381 ggcaagcagg atcaccgctt gcccattcca acataccaaa tcaaaaacaa ttactggtac
     7441 agcatcagcg taccagccca catctctcac tactatcaaa aaccaaaccg ttcagcaaca
     7501 gcgaacggta cacacggaaa aatcaactgg tttacaaata cgaaagacga tcacgctttc
     7561 gtccagcgca aactattacg aaaaacatcc gacgggaaga gcaacagcct tcccgcggcg
     7621 gaaaacctca caaaaacacg acaaacggat gcacgaacac ggcatccgcc gacaacccac
     7681 aaacttacaa ccaggcaaac ggtgcaggat caccgcaccg tacatcaaac acctcagatc
     7741 tcatatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7801 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7861 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7921 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7981 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8041 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8101 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8161 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8221 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8281 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8341 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     8461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8641 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8701 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
     8761 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8821 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8881 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8941 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9001 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9061 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9121 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9181 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     9241 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9301 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9361 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9421 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9481 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9541 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9601 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9661 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9721 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9781 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9841 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9901 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9961 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10021 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10081 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10141 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10201 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10261 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10321 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10381 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10441 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10501 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10561 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10621 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10681 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10741 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10801 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
    10861 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10921 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
    10981 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11041 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    11101 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    11161 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    11221 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    11281 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11341 gttaaatatg tactacaaac ttagtagt
//
