LOCUS       sequence-416805-       10637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 10637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pUB_SMFLuc0---MS2
FEATURES             Location/Qualifiers
     source          1..10637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3213..3236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3243..3266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3309..3332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3552..3575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7027..7615
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7080..7100)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7498..7509)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7690..7923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8042..8060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8128..8232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8233..8301,8302..9093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8451..8470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9264..9852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9753..9772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10006..10023
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10263..10284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10299..10304,10305..10322,10323..10329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10337..10353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10342..10364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10361..10377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10361..10377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10427..10446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
     2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
     2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
     2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
     2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
     2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
     2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
     3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
     3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
     3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
     3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
     3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
     3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
     3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
     3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
     3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
     3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
     3601 agggaggcgg tggatcaggt ggaggaggtt cactgcagga agatgcgaag aatataaaga
     3661 agggacccgc gcctttttat cccctagaag atggaaccgc gggagaacaa ctacataagg
     3721 caatgaagag atatgcacta gtacctggaa caatagcatt tacagatgca catatagaag
     3781 tcgatataac gtatgcggaa tattttgaaa tgtccgtccg tctcgcagaa gcgatgaaga
     3841 gatatgggct aaatacaaat catagaatag tcgtatgtag tgaaaattct cttcaatttt
     3901 ttatgcctgt cctcggggcg ctatttatag gagtcgcagt cgcgcccgcg aatgatatat
     3961 ataatgaacg tgaactcctc aatagtatgg gaatatcgca acctaccgta gtatttgtat
     4021 ccaagaaggg gctccaaaag atactcaatg tccaaaagaa gctccccata atccaaaaga
     4081 tcataataat ggattctaag acggattatc aaggatttca atcgatgtat acgtttgtca
     4141 catctcatct acctcccgga tttaatgaat atgattttgt ccctgaatcc tttgataggg
     4201 ataagacaat agcactaata atgaattcct ctggatctac cggacttcct aagggggtcg
     4261 cacttcctca tagaaccgca tgtgtcagat tttcgcatgc gagagatcct atatttggca
     4321 atcaaataat acctgataca gcgatactta gtgtcgtccc ttttcatcat gggtttggaa
     4381 tgtttactac actcggatat ttcatatgtg gatttcgcgt cgtcctcatg tatagatttg
     4441 aagaagaact ctttctaagg tcccttcaag attataagat acaaagtgcg ctcctcgtcc
     4501 ctaccctatt ctcctttttt gcaaagagta cccttataga taagtatgat ctttctaatc
     4561 tacatgaaat agcatctggg ggagcgcccc tctctaagga agtcggggaa gcggtagcaa
     4621 agaggtttca tctacctggg ataaggcaag gatatgggct cacagaaacc acaagtgcga
     4681 tactaataac acccgaaggg gatgataagc ctggagcggt cgggaaggta gtaccctttt
     4741 ttgaagcgaa ggtcgtagat cttgataccg ggaagacgct tggagtcaat caaagagggg
     4801 aactctgtgt cagaggacct atgataatgt ccggatatgt aaataatccc gaagcgacca
     4861 atgcgctaat agataaggat ggatggctac attctggaga tatagcgtat tgggatgaag
     4921 atgaacattt ttttatagtt gaccgcctaa agtctctcat aaagtataag gggtatcaag
     4981 tagcacccgc agaacttgaa tccatactac tccaacatcc caatatattt gatgcagggg
     5041 tcgcaggact tcccgatgat gatgcggggg aacttcccgc agcggtcgtc gtcctcgaac
     5101 atggaaagac gatgacggaa aaggaaatag tagattatgt cgcaagtcaa gtaacaaccg
     5161 cgaagaagct tcgcggagga gtcgtatttg tcgacgaagt acccaagggg cttaccggaa
     5221 agctcgatgc aagaaagata agagaaatac tcataaaggc aaagaagggg ggaaagatag
     5281 cagtataagc tagctaggcg gactgttact gagctgcgtt ttacaccctt tctttgacaa
     5341 aacctaactt gcgcagaaaa aaaaaaaata agagacaaca ttggcatggc tttgtttttt
     5401 taaatttttt ttaaagtttt tttttttttt tttttttttt aagttttttt gttttgtttt
     5461 ggcgcttttg actcaggatt taaaaactgg aacggtgaag gcgacagcag ttggttggag
     5521 caaacatccc ccaaagttct acaaatgtgg ctgaggactt tgtacattgt tttgtttttt
     5581 ttttttttgg ttttgtcttt ttttaatagt cattccaagt atccatgaaa taagtggtta
     5641 caggaagtcc ctcaccctcc caaaagccac ccccactcct aagaggagga tggtcgcgtc
     5701 catgccctga gtccaccccg gggaaggtga caaccggtaa cctacaaacg ggtggaggat
     5761 caccccaccc gacacttcac aatcaagggg tacaatacac aagggtggag gaacacccca
     5821 ccctccagac acattacaca gaaatccaat caaacagaag caccatcagg gcttctgcta
     5881 ccaaatttat ctcaaaaaac tacaacaagg aatcaccatc agggattccc tgtgcaatat
     5941 acgtcaaacg agggccacga cgggaggacg atcacgcctc ccgaatatcg gcatgtctgg
     6001 ctttcgaatt cagtgcgtgg agcatcagcc cacgcagcca atcagagtcg aatacaagtc
     6061 gactttcgcg aagagcatca gccttcgcgc cattcttaca caaaccacac tctcccctac
     6121 aggaacagca tcagcgttcc tgcccagtac ccaactcaag aaaatttatg tccccatgca
     6181 gcatcagcgc atgggcccca agaatacatc cccaacaaaa tcacatccga gcaccaacag
     6241 ggctcggagt gttgtttctt gtccaactgg acaaaccctc catggaccat caggccatgg
     6301 actctcacca acaagacaaa aactactctt ctcgaagcag catcagcgct tcgaaacact
     6361 cgagcataca ttgtgcctat ttcttgggtg gacgatcacg ccacccatgc tctcacgaat
     6421 ttcaaaacac ggacaaggac gagcaccacc agggctcgtc gttccacgtc caatacgatt
     6481 acttaccttt cgggatcacg atcacggatc ccgcagctac atcacttcca ctcaggacat
     6541 tcaagcatgc acgatcacgg catgctccac aagtctcaac cacagaaact accaaatggg
     6601 ttcagcacca gcgaacccac tcctacctca aacctcttcc cacaaaactg gcaagcagga
     6661 tcaccgcttg cccattccaa cataccaaat caaaaacaat tactggtaca gcatcagcgt
     6721 accagcccac atctctcact actatcaaaa accaaaccgt tcagcaacag cgaacggtac
     6781 acacggaaaa atcaactggt ttacaaatac gaaagacgat cacgctttcg tccagcgcaa
     6841 actattacga aaaacatccg acgggaagag caacagcctt cccgcggcgg aaaacctcac
     6901 aaaaacacga caaacggatg cacgaacacg gcatccgccg acaacccaca aacttacaac
     6961 caggcaaacg gtgcaggatc accgcaccgt acatcaaaca cctcagatct catatcgata
     7021 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7081 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7141 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7201 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7261 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7321 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7381 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7441 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7501 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7561 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     7621 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7681 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7741 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7801 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7861 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7921 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7981 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     8041 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8461 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10021 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10081 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10201 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10261 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10321 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    10381 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10441 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    10501 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    10561 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10621 actacaaact tagtagt
//
