LOCUS       sequence-416812-        4290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Optimal Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pIS1_Opt_Renilla
FEATURES             Location/Qualifiers
     source          1..4290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..786
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          856..988
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     971..990
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2058..2076
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2100..2118
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2124..2142
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2182..2200
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2206..2224
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     polyA_signal    2272..2393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2283..2302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2337..2356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2526..2630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2631..2699,2700..3491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2849..2868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3662..4250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4151..4170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctggccttt tgctcacatg gctcgacaga tctaaatgag tcttcggacc tcgcgggggc
       61 cgcttaagcg gtggttaggg tttgtctgac gcggggggag ggggaaggaa cgaaacactc
      121 tcattcggag gcggctcggg gtttggtctt ggtggccacg ggcacgcaga agagcgccgc
      181 gatcctctta agcacccccc cgccctccgt ggaggcgggg gtttggtcgg cgggtggtaa
      241 ctggcgggcc gctgactcgg gcgggtcgcg cgccccagag tgtgaccttt tcggtctgct
      301 cgcagacccc cgggcggcgc cgccgcggcg gcgacgggct cgctgggtcc taggctccat
      361 ggggaccgta tacgtggaca ggctctggag catccgcacg actgcggtga tattaccgga
      421 gaccttctgc gggacgagcc gggtcacgcg gctgacgcgg agcgtccgtt gggcgacaaa
      481 caccaggacg gggcacaggt acactatctt gtcacccgga ggcgcgaggg actgcaggag
      541 cttcagggag tggcgcagct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt
      601 gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag
      661 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc
      721 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc
      781 gcttaaaagc ttgattcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg
      841 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa
      901 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
      961 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa
     1021 ggctagagta cttaatacga ctcactatag gctagccacc atgaccagca aggtgtacga
     1081 ccccgagcag cgcaagcgca tgatcaccgg cccccagtgg tgggcccgct gcaagcagat
     1141 gaacgtgctg gacagcttca tcaactacta cgacagcgag aagcacgccg agaacgccgt
     1201 gatcttcctg cacggcaacg ccgccagcag ctacctgtgg cgccacgtgg tgccccacat
     1261 cgagcccgtg gcccgctgca tcatccccga cctgatcggc atgggcaaga gcggcaagag
     1321 cggcaacggc agctaccgcc tgctggacca ctacaagtac ctgaccgcct ggttcgagct
     1381 gctgaacctg cccaagaaga tcatcttcgt gggccacgac tggggcgcct gcctggcctt
     1441 ccactacagc tacgagcacc aggacaagat caaggccatc gtgcacgccg agagcgtggt
     1501 ggacgtgatc gagagctggg acgagtggcc cgacatcgag gaggacatcg ccctgatcaa
     1561 gagcgaggag ggcgagaaga tggtgctgga gaacaacttc ttcgtggaga ccatgctgcc
     1621 cagcaagatc atgcgcaagc tggagcccga ggagttcgcc gcctacctgg agcccttcaa
     1681 ggagaagggc gaggtgcgcc gccccaccct gagctggccc cgcgagatcc ccctggtgaa
     1741 gggcggcaag cccgacgtgg tgcagatcgt gcgcaactac aacgcctacc tgcgcgccag
     1801 cgacgacctg cccaagatgt tcatcgagag cgaccccggc ttcttcagca acgccatcgt
     1861 ggagggcgcc aagaagttcc ccaacaccga gttcgtgaag gtgaagggcc tgcacttcag
     1921 ccaggaggac gcccccgacg agatgggcaa gtacatcaag agcttcgtgg agcgcgtgct
     1981 gaagaacgag cagtaattct aggagctcta agaattcgct agcttcccta agtccaacta
     2041 ccaaactggg gattcctggg ccctgaagaa gggcccctcg actagtccaa atacaaactg
     2101 ggccctgaag aagggcccat atagggccct gaagaagggc cctatcgagg atattatctc
     2161 gactaagtcc aactacaaac tgggccctga agaagggccc atatagggcc ctgaagaagg
     2221 gccctatcga ggatattatc tcgagcttgc ggccgcttcg agcagacatg ataagataca
     2281 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2341 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2401 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2461 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc aggtggcact tttcggggaa
     2521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3781 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4261 acgcggcctt tttacggttc ctggcctttt
//
