LOCUS       sequence-416825-        8088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SM(FLAG)-optimal Firefly luciferase - pA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pcDNA3.1_SM-FLuc100-pA
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(100..204)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(279..298)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        470..849
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        850..1053
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1004..1024
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1098..1117
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1098..1116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1142..1151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1151..1174
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1205..1228
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1733..1756
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1763..1786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1799..1822
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1829..1852
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2045..2068
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2072..2095
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2099..2122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3914..3931)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3920..4144
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4190..4618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4277..4296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4487..4508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4627..4647)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4632..4961
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4812..4947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4874..4893
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5028..5822
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5082..5101)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5692..5711
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5996..6117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6033..6052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6087..6106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6166..6182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6166..6182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6179..6201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6190..6206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6214..6220,6221..6238,6239..6244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6259..6280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6397..6414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6568..7156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6648..6667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7327..8088,1..30,31..99))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7950..7969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
       61 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
      241 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
      301 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      361 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      421 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      481 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      541 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      601 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      661 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      721 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      781 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      841 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      901 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      961 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1021 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     1081 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgcggc
     1141 cgccaccatg gactacaagg acgacgacga caaaggtgac tacaaagatg atgacgataa
     1201 aggcgactat aaggacgatg acgacaaggg cggaaactca ctgatcaagg aaaacatgcg
     1261 gatgaaggtg gtgatggagg gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg
     1321 agagggaaac ccgtacatgg gaactcagac catgcgcatt aaggtcatcg aaggaggtcc
     1381 gctgccgttc gctttcgata tcctggccac ttcgttcgga ggagggtcgc gcacgttcat
     1441 caagtacccg aagggaatcc cggacttctt taagcagtca ttcccggaag gattcacttg
     1501 ggaacgggtg acccggtatg aagatggagg tgtggtgact gtcatgcaag atacttcgct
     1561 ggaggatggg tgcctcgtgt accacgtcca agtccgcgga gtgaatttcc cgtccaacgg
     1621 accagtgatg cagaaaaaga cgaagggttg ggaacctaat actgaaatga tgtaccccgc
     1681 agacggaggg ctgaggggct acacccacat ggcgctgaag gtcgacggag gagattacaa
     1741 ggatgacgac gataagcaac aagattacaa agacgatgat gacaagggcc agcagggcga
     1801 ctacaaggac gacgacgaca agcagcagga ctacaaagat gacgatgata aaggaggagg
     1861 acatctgtcc tgttcgttcg tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa
     1921 gatgccgggc attcatgccg tcgaccaccg cctggagcgg ctcgaagaat cagacaatga
     1981 gatgttcgtc gtgcaaagag aacatgccgt ggccaagttc gcgggactgg gaggcggtgg
     2041 aggcgattac aaagacgatg atgacaaggg tgactataaa gacgacgatg acaaagggga
     2101 ttacaaggat gatgatgata agggaggcgg tggatcaggt ggaggaggtt cactgcagga
     2161 ggacgccaaa aacattaaaa aaggcccggc tccattctac ccgctggagg acggtactgc
     2221 tggcgagcag ctgcacaaag ctatgaaacg atacgcacta gttccgggta ctattgcttt
     2281 cactgacgct cacatcgagg tggacatcac ttacgctgag tacttcgaga tgagcgttcg
     2341 gttggccgag gctatgaaac gatacggcct gaacactaac caccgaatcg tggtgtgctc
     2401 agagaactca ttacagttct tcatgccggt gttgggcgcc ttattcatcg gcgttgctgt
     2461 tgccccagcc aacgacattt acaacgagcg agagttgtta aactcaatgg gcatttcaca
     2521 gccaactgtg gtgttcgttt caaaaaaagg cttgcagaaa attttgaacg tgcagaaaaa
     2581 attaccaatc atccagaaaa ttattatcat ggacagcaaa actgactacc agggtttcca
     2641 gagcatgtac actttcgtta cttcacactt gccaccgggt ttcaacgagt acgacttcgt
     2701 gccagagagc ttcgaccgag acaaaactat tgccctgatc atgaactcat caggctcaac
     2761 tggtctgccg aaaggtgttg ctctgccgca ccggactgcc tgcgtgcgat tctcacacgc
     2821 ccgggaccca attttcggca accagatcat tccggacact gccattttaa gcgttgttcc
     2881 attccaccac ggtttcggca tgttcactac tctgggttac ttcatatgcg gtttccgagt
     2941 tgtgttaatg taccggttcg aggaggagct gttcctgcga agcttgcagg actacaaaat
     3001 tcagtcagct ctgctggtgc caactctgtt cagcttcttc gccaaaagca ctctgattga
     3061 caaatacgac ttatcaaact tacacgagat tgcttcaggt ggcgctccgt tatcaaaaga
     3121 ggttggcgag gccgttgcca aacgattcca cctgccaggt atccgacagg gttacggttt
     3181 gactgagact acttcagcta ttctgattac tccggagggt gacgacaaac cgggcgctgt
     3241 gggtaaagtt gttccattct tcgaggctaa agttgtggac ctggacactg gtaaaactct
     3301 gggcgttaac cagcgaggcg agctgtgcgt gcgaggtccg atgattatga gcggttacgt
     3361 gaacaacccg gaggctacta acgccttgat tgacaaagac ggttggttac acagcggtga
     3421 catcgcttac tgggacgagg acgagcactt cttcatcgtt gaccggctga aatcactgat
     3481 taaatacaaa ggctaccagg tggctccggc tgagttggag tcaatcttgt tacagcaccc
     3541 aaacatcttc gacgctggtg tggctggtct gccggacgac gacgccggtg agttaccagc
     3601 cgccgttgtt gttttggagc acggtaaaac tatgactgag aaagagatcg tggactacgt
     3661 tgccagccag gttactactg ctaaaaaatt gcggggtggc gttgtgttcg tcgacgaggt
     3721 tccgaaaggt ttaactggca aattggacgc tcgaaaaatc cgagagatcc tgatcaaagc
     3781 caaaaaaggc ggcaaaatcg ccgtgtaagg taccgagctc ggatccacta gtccagtgtg
     3841 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     3901 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3961 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4021 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4081 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4141 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4621 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4861 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4981 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5641 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5821 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5881 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5941 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6121 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6181 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6241 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6301 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6361 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7621 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7921 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagc
//
