LOCUS       sequence-41690-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Mattingly RR, Macara IG
  TITLE     Phosphorylation-dependent activation of the Ras-GRF/CDC25Mm exchange
            factor by muscarinic receptors and G-protein beta gamma subunits.
  JOURNAL   Nature. 1996 Jul 18. 382(6588):268-72.
  PUBMED    8717044
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1996 Jul 18. 382(6588):268-72."
COMMENT     Sequence Label: pKH3
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        187..205
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     187..204
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     218..237
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             298..324
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             331..357
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             364..390
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      427..436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    440..574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(477..496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     531..550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
       61 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      121 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      181 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      241 cactcccagg tccaactgca cctcggttct aagcttctgc aggtcgactc tagaatgtac
      301 ccatacgatg ttccagatta cgctggatct tacccatacg atgttccaga ttacgctgga
      361 tcttacccat acgatgttcc agattacgct ggatccgaat tcaatcgata gatagctgag
      421 tcgatggccg ccatggccca acttgtttat tgcagcttat aatggttaca aataaagcaa
      481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      541 caaactcatc aatgtatctt atcatgtctg gatcgggaat taattcggcg cagcaccatg
      601 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
      661 gctgtggaat gtgtgtcagt ta
//
