LOCUS       sequence-41691-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   El Kasmi KC, Smith AM, Williams L, Neale G, Panopoulos AD, Watowich 
            SS, Hacker H, Foxwell BM, Murray PJ
  TITLE     Cutting edge: A transcriptional repressor and corepressor induced by
            the STAT3-regulated anti-inflammatory signaling pathway.
  JOURNAL   J Immunol. 2007 Dec 1;179(11):7215-9.
  PUBMED    18025162
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2007-12-1"; volume: "179(11)"; pages: "7215-9"
COMMENT     Sequence Label: pMIY-mETV3-Flag
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..574
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(184..203)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     413..430
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(615..638)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtattat cgtgtttttc aaaggaaaac cacgtccccg
       61 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
      121 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
      181 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
      241 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
      301 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
      361 cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
      421 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
      481 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
      541 cggccttatt ccaagcggct tcggccagta acgttagggg gggggcggaa ttgatcccgc
      601 tcgagcaatt gttacttgtc atcgtcgtcc ttgtagtcag catcagcggc agcagtggcc
      661 gttgtggcca ggccctgggg
//
