LOCUS       sequence-416922-        9068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Conway JRW, Isomursu A, Follain G, Harma V, Jou-Olle E, Pasquier N, 
            Valimaki EPO, Rantala JK, Ivaska J
  TITLE     Defined extracellular matrix compositions support 
            stiffness-insensitive cell spreading and adhesion signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2304288120. doi: 
            10.1073/pnas.2304288120. Epub 2023 Oct 16.
  PUBMED    37844244
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-10-24"; volume: "120(43):e2304288120. 
            doi"; pages: " 10.1073/pnas.2304288120. Epub 2023 Oct 16"
COMMENT     Sequence Label: pDEST-mCherry-ARHGEF2
FEATURES             Location/Qualifiers
     source          1..9068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      552..561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             558..1265
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(703..721)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     971..990
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4207..4231)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4263..4282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4264..4282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4439..4573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4476..4495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4530..4549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4699..5154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4786..4805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4996..5017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5271..5628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5271..5462
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5294..5314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5479..5614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5679..6473
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5733..5752)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6343..6362
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6537..6585
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6626..6645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6745..6767
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6891..6995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6996..7064,7065..7856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7214..7233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8027..8615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8516..8535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8769..8786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(8860..8964)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgtcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      541 cgctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      601 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      661 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      721 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      781 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      841 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      901 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      961 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     1021 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     1081 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1141 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     1201 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     1261 acaagtccgg actcagatct cgagcttcta gatcaacaag tttgtacaaa aaagcaggct
     1321 ccaccatgaa ggaagccaag gatgcccgct ataccaatgg gcacctcttc accaccattt
     1381 cagtttcagg catgaccatg tgctatgcct gtaacaagag catcacagcc aaggaagccc
     1441 tcatctgccc aacctgcaat gtgactatcc acaaccgctg taaagacacc ctcgccaact
     1501 gtaccaaggt caagcagaag caacagaaag cggccctgct gaagaacaac accgccttgc
     1561 agtccgtttc tcttcgaagt aagacaacca tccgggagcg gccaagctcg gccatctacc
     1621 cctccgacag cttccggcag tccctcctgg gctcccgccg tggccgctcc tccttgtctt
     1681 tagccaagag tgtttctacc accaacattg ctggacattt caatgatgag tctcccctgg
     1741 ggctgcgccg gatcctctca cagtccacag actccctcaa catgcggaac cgaaccctat
     1801 ccgtggaatc cctcattgac gaagcagagg taatctacag tgagctgatg agtgactttg
     1861 agatggatga gaaggacttt gcagctgact cttggagtct tgctgtggac agcagcttcc
     1921 tgcagcagca taaaaaggag gtgatgaagc agcaagatgt catctatgag ctaatccaga
     1981 cagagctgca ccatgtgagg acactgaaga tcatgacccg cctcttccgc acggggatgc
     2041 tggaagagct acacttggag ccaggagtgg tccagggcct gttcccctgc gtggacgagc
     2101 tcagtgacat ccatacacgc ttcctcagcc agctattaga acgccgacgc caggccctgt
     2161 gccctggcag cacccggaac tttgtcatcc atcgcttggg tgatctgctc atcagccagt
     2221 tctcaggtcc tagtgcggag cagatgtgta agacctactc ggagttctgc agccgccaca
     2281 gcaaggcctt aaagctctat aaggagctgt acgcccgaga caaacgcttc cagcaattca
     2341 tccggaaagt gacccgcccc gccgtgctca agcggcacgg ggtacaggag tgcatcctgc
     2401 tggtgactca gcgcatcacc aagtacccgt tactcatcag ccgcatcctg cagcattccc
     2461 acgggatcga ggaggagcgc caggacctga ccacagcact ggggctagtg aaggagctgc
     2521 tgtccaatgt ggacgagggt atttatcagc tggagaaagg ggcccgtctg caggagatct
     2581 acaaccgcat ggaccctcgg gcccaaaccc cagtgcctgg caagggcccc tttggccgag
     2641 aggaacttct gaggcgcaaa ctcatccacg atggctgcct gctctggaag acagcgacgg
     2701 ggcgcttcaa agatgtgcta gtgctgctga tgacagatgt actggtgttt ctccaggaaa
     2761 aggaccagaa gtacatcttt cctaccctgg acaagccttc agtggtatcg ctgcagaatc
     2821 taatcgtacg agacattgcc aaccaggaga aagggatgtt tctgatcagc gcagccccac
     2881 ctgagatgta cgaggtgcac acagcatccc gggatgaccg gagcacctgg atccgggtca
     2941 ttcagcagag cgtgcgcaca tgcccatcca gggaggactt ccccctgatt gagacagagg
     3001 atgaggctta cctgcggcga attaagatgg agttgcagca gaaggaccgg gcactggtgg
     3061 agctgctgcg agagaaggtc gggctgtttg ctgagatgac ccatttccag gccgaagagg
     3121 atggtggcag tgggatggcc ctgcccaccc tgcccagggg ccttttccgc tctgagtccc
     3181 ttgagtcccc tcgtggcgag cggctgctgc aggatgccat ccgtgaggtg gagggtctga
     3241 aagacctgct ggtggggcca ggagtggaac tgctcttgac accccgagag ccagccctgc
     3301 ccttggaacc agacagcggt ggtaacacga gtcctggggt cactgccaat ggtgaggcca
     3361 gaaccttcaa tggctccatt gaactctgca gagctgactc agactctagc cagagggatc
     3421 gaaatggaaa tcagctgaga tcaccgcaag aggaggcgtt acagcgattg gtcaatctct
     3481 atggacttct acatggccta caggcagctg tggcccagca ggacactctg atggaagccc
     3541 ggttccctga gggccctgag cggcgggaga agctgtgccg agccaactct cgggatgggg
     3601 aggctggcag ggctggggct gcccctgtgg cccctgaaaa gcaggccacg gaactggcat
     3661 tactgcagcg gcaacatgcg ctgctgcagg aggagctacg gcgctgccgg cggctaggtg
     3721 aagaacgggc aaccgaagct ggcagcctgg aggcccggct ccgggagagt gagcaggccc
     3781 gggcactgct ggagcgtgag gccgaagagg ctcgaaggca gctggccgcc ctgggccaga
     3841 ccgagccact cccagctgag gccccctggg cccgcagacc tgtggatcct cggcggcgca
     3901 gcctccccgc aggcgatgcc ctgtacttga gtttcaaccc cccacagccc agccgaggca
     3961 ctgaccgcct ggatctacct gtcactactc gctctgtcca tcgaaacttt gaggaccgag
     4021 agaggcagga actggggagc cccgaagagc ggctgcaaga cagcagtgac cctgacactg
     4081 gcagcgagga ggaaggtagc agccgtctgt ctccgcccca cagtccacga gactttacca
     4141 gaatgcagga catcccggag gagacggaga gccgcgacgg ggaggctgta gcctccgaga
     4201 gctaggaccc agctttcttg tacaaagtgg ttgatcgcgt gcatgcgacg tcatagctct
     4261 ctccctatag tgagtcgtat tataagctag gcactggccg tcgttgatgg gatccaccgg
     4321 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4381 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4561 tcatgtctgg atcgatcctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4621 gtattggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4681 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4741 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4801 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4861 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4921 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4981 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
     5161 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     5221 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     5281 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5341 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5401 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5461 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5521 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5581 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct
     5641 tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa gatggattgc
     5701 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5761 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5821 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5881 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5941 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6001 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6061 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6121 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6181 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6241 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6301 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6361 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6421 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6481 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata
     6541 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa
     6601 ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     6661 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6721 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6781 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6961 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7081 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7141 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7201 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7261 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7321 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7381 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7441 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7501 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7561 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7621 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8161 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8821 tccccgcgcg ttggccgatt cattaatgca gagcttgcaa ttcgcgcttt ttcaatatta
     8881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9001 aaccattatt atcatgacat taacctataa aaataggcgt attacgaggc cctttcacgc
     9061 attagatg
//
