LOCUS       sequence-416932-       11310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression cassette for the E3-DART ligase 
            containing a nuclear localization signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry...
FEATURES             Location/Qualifiers
     source          1..11310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2011..2031
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2032..2052
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2053..2073
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2074..2100
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2222..2233
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      4482..4734
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4769..4787)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4975..4999
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6300..6929
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7358..8431
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8497..8691
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8927..8949)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9035..9175
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9090..9109
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9190..9207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9361..9949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9441..9460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10037..10852)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10037..10852)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10760..10779
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11285..11309
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagaatg cctaagaaaa agagaaaggt tcctaagaaa
     2041 aagagaaagg ttcctaaaaa gaaaagaaag gtttacccat acgatgttcc agattacgct
     2101 gagtatgatg ctgtctggtc aaaatgggaa agggatgcac cagcaggaga gtcacctgga
     2161 agagcagcag tggttcagga aatgcgtgat tgccttaata acggaaatcc agtgcttaac
     2221 gtgggagctt caggtcttac cacccttcca gaccgtcttc caccgcatat tacaacactt
     2281 gttattccag ataataatct cacctctttg cctgagttgc cagaaggact tagagagctt
     2341 gaggtgtctg gaaaccttca gcttacatct cttccatctc ttccgcaggg tcttcagaag
     2401 ttgtgggcct ataacaattg gcttgcctct cttccgactt tgccgccagg acttggggat
     2461 cttgctgtct ctaataacca gcttacctct cttccggaga tgccgccagc acttagagag
     2521 cttagggtgt ctggtaacaa ccttacctct cttccggctc ttccgtcagg acttcagaag
     2581 ctttgggcat ataacaatag attgacctct ttgccagaga tgtcaccagg tcttcaggaa
     2641 cttgatgtct ctcataatca gcttaccaga ttgccgcaat ctctcactgg tctttcttct
     2701 gctgcaagag tttatcttga cgggaatcca ctttctgtta gaactcttca ggctcttaga
     2761 gacatcattg gacattcagg aatcaggata cacttcgata tggctgggcc ttctgtccct
     2821 agagaagcca gagcacttca ccttgctgtc gctgactggc ttacttctgc aagagagggg
     2881 gaagctgccc aggcagacag atggcaggct ttcggacttg aagataacgc cgccgccttc
     2941 tctcttgtcc ttgacagact tcgtgagact gaaaacttca aaaaagacgc tggatttaag
     3001 gcacagatat catcttggct tacacaactt gctgaagatg ctgctcttag agcaaaaacc
     3061 tttgccatgg caacagaggc aacatcaacc tgcgaggaca gagtcacaca tgcccttcac
     3121 cagatgaata acgttcaact tgttcataat gcagaaaaag gggaatacga caacaatctc
     3181 caggggcttg tttctactgg gcgtgagatg ttcagacttg caacacttga acagattgcc
     3241 agagaaaaag ccggaacact tgctcttgtc gatgacgttg aggtctatct tgctttccag
     3301 aataagctta aggaatcact tgagcttacc tctgtgactt cagaaatgcg tttctttgac
     3361 gtttctggag tgactgtttc agaccttcag gctgctgagc ttcaggtgaa aaccgctgaa
     3421 aactctgggt tcagtaaatg gatacttcag tgggggccgc ttcactctgt gcttgaaaga
     3481 aaagtgccgg aaagatttaa cgctcttcgt gaaaagcaaa tatctgatta tgaagacact
     3541 tacagaaagc tttatgacga agtgcttaaa tcttctgggc ttgtcgacga taccgatgca
     3601 gaacgtacta tcggagttag tgctatggat agtgctaaaa aagaatttct tgatggactt
     3661 agagctcttg tggatgaggt gcttggttct tatcttacag ccagatggcg tcttaacttc
     3721 ggaggaggtg gctcatctgg cggaggtatg gtgagtaagg gtgaagaaga taatatggca
     3781 ataattaaag aatttatgcg ttttaaagtc catatggaag gatcagtcaa tggtcatgaa
     3841 ttcgaaattg agggggaagg tgaaggaagg ccgtatgagg ggactcagac cgctaaactc
     3901 aaggtgacca agggtgggcc tttgccgttt gcatgggata tcctctctcc acaatttatg
     3961 tatggatcaa aggcttacgt caagcacccg gctgacatac ctgactatct taaactctct
     4021 ttcccggaag gttttaagtg ggaaagagtg atgaatttcg aagatggtgg ggttgtgacc
     4081 gttacacaag attcaagtct gcaggatgga gagtttattt acaaggttaa actcagaggt
     4141 accaatttcc cgtctgacgg gcctgtgatg caaaaaaaga ctatggggtg ggaggcctct
     4201 tcagaaagaa tgtatcctga ggatggagca ctcaagggtg aaattaaaca aagattgaag
     4261 cttaaagatg gaggtcatta tgacgccgaa gtgaaaacta catataaggc aaagaagcca
     4321 gtccaattgc cgggagcata taatgtgaat attaaactcg atatcacctc tcacaatgaa
     4381 gattatacta tcgtcgaaca gtatgaaagg gccgaaggtc gtcattcaac cggaggtatg
     4441 gatgagctct ataagtgagc ttggaatgga tcttcgatcc cgatcgttca aacatttggc
     4501 aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc atataatttc
     4561 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     4621 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     4681 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     4741 tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4801 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4861 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4921 ccctgataaa tgcttcaata atgggaccga ctcgcgctgt catgagacga attctgacag
     4981 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     5041 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     5101 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     5161 acgttcagtg cagccgtcat ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     5221 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     5281 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     5341 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     5401 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     5461 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     5521 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     5581 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     5641 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     5701 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     5761 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     5821 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     5881 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     5941 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     6001 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     6061 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacc tctcaaccgt gcggctgcat
     6121 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     6181 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     6241 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     6301 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     6361 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     6421 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     6481 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     6541 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     6601 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     6661 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     6721 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     6781 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     6841 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     6901 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     6961 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     7021 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     7081 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     7141 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     7201 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     7261 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     7321 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     7381 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     7441 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     7501 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     7561 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     7621 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     7681 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     7741 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     7801 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     7861 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     7921 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     7981 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     8041 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     8101 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     8161 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     8221 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     8281 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     8341 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     8401 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     8461 cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa
     8521 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     8581 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     8641 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     8701 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     8761 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     8821 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     8881 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     8941 agctcccggt gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     9001 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     9061 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9121 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     9181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9781 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10021 catgcattct aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
    10081 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
    10141 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
    10201 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
    10261 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
    10321 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
    10381 tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat
    10441 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
    10501 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg
    10561 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
    10621 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
    10681 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg
    10741 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
    10801 tggaatttaa tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac
    10861 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    10921 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    10981 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    11041 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    11101 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    11161 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    11221 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    11281 ctggtggcag gatatattgt ggtgtaaaca
//
