LOCUS       sequence-416934-       10676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression cassette for RHD6 fused with GFP and a 
            mutant form of the HR1b degron .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR...
FEATURES             Location/Qualifiers
     source          1..10676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1298..1300,1301..1303,1304..2014)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1298..1300,1301..1303,1304..2014)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1343..1364)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1604..1623)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1951..1972
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4341..4365
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5666..6295
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6724..7797
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7863..8057
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8293..8315)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8401..8541
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8456..8475
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8556..8573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8727..9315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8807..8826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9403..10218)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9403..10218)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10126..10145
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    10651..10675
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca
     1021 acaaacaaca ttacaattta ctattctagt cgaaatggct actaatcttt ctagagttgc
     1081 tggacttgaa aagcaacttg ctattgaact taaggttaag caaggtgctg aaaatatgat
     1141 tcaaacttat tctaatggat ctactaagga tagaaagctt cttcttactg ctcaacaaat
     1201 gcttcaagat tctaagacta agattgatat tattgcaatg caacttgcaa gagctcttca
     1261 agctggtcaa cttgaaaatc aagctgctcc tggttcgatg gtgagcaagg gcgaggagct
     1321 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1381 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1441 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1501 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1561 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1621 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1681 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1741 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1801 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1861 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1921 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1981 cgggatcact ctcggcatgg acgagctgta caagggaggt ggctcatctg gcggaggtgg
     2041 ttcgatggca ctcgttaatg accatcccaa cgagactaat tacttgtcaa aacaaaattc
     2101 ctcctcttcc gaagatctct cctcgccggg actggatcag ccagatgcag cttatgccgg
     2161 tggaggagga ggaggaggct cggcttcgag cagtagcacg atgaattcag atcatcaaca
     2221 acatcagggg tttgtatttt acccatccgg tgaagatcat cacaactctt tgatggattt
     2281 caacggatca tcatttctta actttgatca tcacgagagc tttcctcctc cagccataag
     2341 ctgtggtggt agtagcggtg ggggcggctt ctccttcttg gagggcaaca acatgagcta
     2401 cggcttcaca aactggaatc atcaacatca tatggatatt attagcccta gatccaccga
     2461 aactccccaa ggccagaaag actggttata ttctgattca actgttgtaa ccactggttc
     2521 tagaaacgag tctctttcgc ctaaatccgc tggaaacaaa cgttctcaca cggtaattac
     2581 aaacaacaaa gtcttggtga atatttttca tataagatgt caatgaaaga tcgttgatat
     2641 atactactat caatttgaat tatggatata tagggagaga gcactcaacc gtcgaagaaa
     2701 ctgagtagcg gtgtgaccgg aaagaccaag cctaagccaa caacttcacc taaagatcca
     2761 caaagcctag cagccaaggt ttgctttctt gacattaatt tacattcatt aaaataaaac
     2821 tcaatctctc aacacatatt tacatatgtt ttattttgtt cagaatcgaa gagaaaggat
     2881 aagtgaacgt cttaagatat tgcaagaact tgttcccaat ggcaccaagg tacacatcca
     2941 tataaccatt tatgacagat ttattatatt acttattctc atgataataa tatttgttat
     3001 gtttcacagg ttgatttggt gacaatgctt gaaaaggcta ttagttatgt caagttcctt
     3061 caagtacaag ttaaggtata ttagttatga caaacatccc tcaaattaat tttcaggatt
     3121 gatttaatta ccatatttat cgaattatta cgtaacacgt attgatataa tctattgttt
     3181 gttaggtatt agcgaccgat gagttttggc cggctcaagg aggaaaagct cctgacattt
     3241 ctcaagttaa agacgccatt gatgccattc tctcctcatc acaacgagac aggaattcga
     3301 atctgatcac caattaatga agggttttat cattaataaa tcagtttatc aaacattaat
     3361 tacgtacgtt gtaataatat cgggggaaac aatgattctc tcgattataa atcccacgta
     3421 aattttgaaa tatgatccaa agagaagaca atcgagatta tgtaacgtta gattatgtga
     3481 aaatcgagaa gttcttaatg catctataac gaagagaaga caagagacat tgaattcacc
     3541 gatgatcagt ctgatatggg tccacacggg gatggaatct ttaactagta aataagtgtt
     3601 aaaaacataa atatgttttt caatttcttt ttctgtttta attgtatttt gtgtgtttta
     3661 gtctacggaa tacagatctt ttatttaaac tccaagaaat gagtaataaa attcttcact
     3721 tttgtttttt ccttgaggtt tcagtttcag agaatctatc tttttgtcat ctttctttgt
     3781 ctatttgagc ttggctttat taaagctagc tcttgagcga catgcttatt tatttaaaat
     3841 tcgtcaaaag tttgtcacac atattatagg gtccctatat agattgcaat gcatagtatt
     3901 ttttgaaaaa aaagataaac tatgaatttc tattaccaaa atagaccatt tttttattta
     3961 taattgctct aggacgacga gttcttctgt gcaaaacatt ttcacattct ctgtatgaat
     4021 gatcaaaaca taatgttaac ctagtttgct tggctaaaac attttatgat ataattttgg
     4081 tacactaaaa tatgtatttt cgaaaatata aacattttta tatgtaggta aataagtatt
     4141 caaccaaaag ttgagaaatc ttaaaatcag tgatgaatag catatatttt ttttatcaat
     4201 taattgtagg ataaccatac actgatacac atcagcacct ttatcgtata aaattatttc
     4261 tcaaattaag tatgagatta tattaattga ttggacgaca tacctaagat ctcgatttat
     4321 cagccgcttg agacgaagct tgacaggata tattggcggg taaacctaag agaaaagagc
     4381 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     4441 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     4501 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcatctga aaacgacatg
     4561 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     4621 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     4681 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     4741 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     4801 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     4861 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     4921 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     4981 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     5041 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
     5101 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     5161 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     5221 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     5281 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     5341 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     5401 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     5461 cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     5521 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     5581 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     5641 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     5701 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     5761 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     5821 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     5881 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     5941 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     6001 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     6061 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     6121 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     6181 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     6241 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     6301 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     6361 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     6421 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     6481 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     6541 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     6601 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     6661 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     6721 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     6781 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     6841 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     6901 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     6961 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     7021 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     7081 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     7141 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     7201 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     7261 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     7321 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     7381 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     7441 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     7501 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     7561 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     7621 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     7681 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     7741 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     7801 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt
     7861 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     7921 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     7981 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     8041 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     8101 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     8161 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     8221 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     8281 tgacggtgaa aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc
     8341 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     8401 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     8461 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     8521 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9361 gaaaactcac gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag
     9421 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     9481 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     9541 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     9601 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     9661 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     9721 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
     9781 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     9841 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     9901 tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     9961 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
    10021 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
    10081 cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
    10141 atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc
    10201 ccgttgaata tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa
    10261 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    10321 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    10381 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
    10441 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
    10501 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
    10561 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    10621 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaaca
//
