LOCUS       sequence-416935-        2373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b 
            PE-eVLP production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: COM-epegRNA-Dnmt1
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..330
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     82..102
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     253..272
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(624..1212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(704..723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1383..2174,2175..2243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2006..2025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2244..2348)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc cgggctggag ctgttcgcgc gtttaagagc
      361 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttggctgaat
      421 gcctgcgagc atcccaccca agtggcaccg agtcggtgct gcaagatgcg ttggcgaaca
      481 gctccaggat aatatcgcgg ttctatctag ttacgcgtta aaccaactag aattttttaa
      541 gcttgggccg ctcgaggtac ctctctacat atgacatgtg agcaaaaggc caggaaccgt
      601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1021 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1321 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1381 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1441 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1501 cccagtgctg caatgatacc gcgagatcca cgctcaccgg ctccagattt atcagcaata
     1561 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1621 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1681 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1741 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1801 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1861 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1921 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1981 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2041 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2101 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2161 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2221 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2281 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2341 gttccgcgca catttccccg aaaagtgcca cct
//
