LOCUS       sequence-416942-        3792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homeobox A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Sakai Y, Shaw CA, Dawson BC, Dugas DV, Al-Mohtaseb Z, Hill DE, 
            Zoghbi HY
  TITLE     Protein interactome reveals converging molecular pathways among 
            autism disorders.
  JOURNAL   Sci Transl Med. 2011 Jun 8;3(86):86ra49. doi: 
            10.1126/scitranslmed.3002166.
  PUBMED    21653829
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2011-06-8"; volume: "3(86):86ra49. doi"; pages: " 
            10.1126/scitranslmed.3002166"
COMMENT     Sequence Label: pENTR-HOXA1
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             324..353
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(1135..1234)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1251..1270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1252..1270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1275..1291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1275..1291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1722..2513
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2606..3194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3095..3114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3348..3365
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3524..3551)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3643..3729)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3778..3792,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3792,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catggacaat gcaagaatga actccttcct ggaatacccc atacttagca
      181 gtggcgactc ggggacctgc tcagcccgag cctacccctc ggaccatagg attacaactt
      241 tccagtcgtg cgcggtcagc gccaacagtt gcggcggcga cgaccgcttc ctagtgggca
      301 ggggggtgca gatcggttcg ccccaccacc accaccacca ccaccatcac cacccccagc
      361 cggctaccta ccagacttcc gggaacctgg gggtgtccta ctcccactca agttgtggtc
      421 caagctatgg ctcacagaac ttcagtgcgc cttacagccc ctacgcgtta aatcaggaag
      481 cagacgtaag tggtgggtac ccccagtgcg ctcccgctgt ttactctgga aatctctcat
      541 ctcccatggt ccagcatcac caccaccacc agggttatgc tgggggcgcg gtgggctcgc
      601 ctcaatacat tcaccactca tatggacagg agcaccagag cctggccctg gctacgtata
      661 ataactcctt gtcccctctc cacgccagcc accaagaagc ctgtcgctcc cccgcatcgg
      721 agacatcttc tccagcgcag acttttgact ggatgaaagt caaaagaaac cctcccaaaa
      781 cagggaaagt tggagagtac ggctacctgg gtcaacccaa cgcggtgcgc accaacttca
      841 ctaccaagca gctcacggaa ctggagaagg agttccactt caacaagtac ctgacgcgcg
      901 cccgcagggt ggagatcgct gcatccctgc agctcaacga gacccaagtg aagatctggt
      961 tccagaaccg ccgaatgaag caaaagaaac gtgagaagga gggtctcttg cccatctctc
     1021 cggccacccc gccaggaaac gacgagaagg ccgaggaatc ctcagagaag tccagctctt
     1081 cgccctgcgt tccttccccg gggtcttcta cctcagacac tctgactacc tcccacccaa
     1141 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1201 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1261 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1321 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     1381 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1441 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1501 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1561 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1621 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1681 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     1741 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1801 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     1861 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     1921 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     1981 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2041 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2101 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2161 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2221 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2281 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2341 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2401 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2461 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2521 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3241 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3301 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3361 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3421 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3481 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3541 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3601 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3661 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3721 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     3781 agtcacgacg tt
//
