LOCUS       sequence-417079-        9588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing shRNA targeting Ctnnd2 N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-shCtnnd2.1-GFP
FEATURES             Location/Qualifiers
     source          1..9588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        286..534
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     286..306
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     457..476
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    631..748
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1115..1418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             1702..1711
                     /estimated_length=10
     intron          1835..2851
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2775..2797
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2859..2878
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2928..2937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2934..2936,2937..2939,2940..3653)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2934..2936,2937..2939,2940..3650)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2979..3000)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3240..3259)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3587..3608
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3673..3706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3762..4350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3815..3835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4233..4244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4353..4369)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4354..4370)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4559..4792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4864..4985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4901..4920)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4955..4974
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5025..5160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5180..5199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5181..5199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5209..5226)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5209..5225)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5218..5240)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5367..5822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5454..5473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5664..5685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5848..5952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5953..6021,6022..6813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6171..6190)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6984..7572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7473..7492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7726..7743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7860..7881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7896..7901,7902..7919,7920..7926)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7934..7950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7939..7961
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7958..7974
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7958..7974
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7993..8013
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7995..8013
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8041..8267
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8268..8448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8495..8620
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9114..9347
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9532..9576
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
       61 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
      121 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
      181 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
      241 cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc
      301 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
      361 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      421 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      481 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggccag
      541 gcttaaacac ctccaatctc gagattggag gtgtttaagc ctggtttttg aattctcgac
      601 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      661 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      721 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      781 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
      841 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
      901 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
      961 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     1021 gccggacgtg acaaacggaa gccgcacgtc tcactagtta ttaatagtaa tcaattacgg
     1081 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1141 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1201 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1261 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1321 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1381 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     1441 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     1501 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccgg gcgggggggg
     1561 gggggggggg gggggggggg gggggggggg gggggggggg gggggggcgg cggggggggg
     1621 ggggcgccgc aagtgttttt ttttttgggg gcggcggggg gggggggccc cttaaaaaaa
     1681 caggaggccg gggggggggg gnnnnnnnnn ncggggcggg gcgaggcgga gaggtgcggc
     1741 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     1801 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
     1861 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     1921 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     1981 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     2041 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     2101 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     2161 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     2221 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     2281 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     2341 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     2401 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
     2461 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     2521 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     2581 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     2641 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     2701 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     2761 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     2821 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     2881 gtctcatcat tttggcaaag aattcgcggc cgctagcgct accggtcgcc accatggtga
     2941 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3001 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3061 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3121 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3181 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3241 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3301 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3361 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3421 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3481 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3541 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3601 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taggaattcg
     3661 tcgagggacc taataacttc gtatagcata cattatacga agttatacat gtttaagggt
     3721 tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca
     3781 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3841 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3901 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3961 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4021 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4081 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4141 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4201 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4261 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4321 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
     4381 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     4441 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     4501 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4561 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4621 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4681 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4741 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     4801 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4861 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4981 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     5041 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5101 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5161 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     5221 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     5281 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5341 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     5401 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5461 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5521 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5581 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5641 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5701 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5761 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     5821 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5881 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5941 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6001 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6061 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6121 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6181 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6241 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6301 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6361 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6421 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6481 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6541 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6601 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6661 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6721 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6781 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7021 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7081 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7141 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7201 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7261 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7321 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7381 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7441 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7501 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7561 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7621 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7681 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7741 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7801 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7861 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7921 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7981 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     8041 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     8101 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     8161 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     8221 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     8281 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8341 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8401 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8461 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8521 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8581 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8641 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8701 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8761 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8821 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     8881 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     8941 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9001 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9061 ccaccaaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9121 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9181 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9241 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9301 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9361 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9421 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9481 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9541 caagaattat tggaattaga taaatgggca agtttgtgga attggttt
//
