LOCUS       sequence-417108-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses mouse NINJ1 tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Ramos S, Hartenian E, Santos JC, Walch P, Broz P
  TITLE     NINJ1 induces plasma membrane rupture and release of 
            damage-associated molecular pattern molecules during ferroptosis.
  JOURNAL   EMBO J. 2024 Apr;43(7):1164-1186. doi: 10.1038/s44318-024-00055-y. 
            Epub 2024 Feb 23.
  PUBMED    38396301
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00055-y"; journalName: "EMBO J"; date: 
            "2024-04"; volume: "43"; issue: "7"; pages: "1164-1186"
COMMENT     Sequence Label: pLVX-mNINJ1-GFP
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             102..113
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     511..531
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(598..1617)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(598..1617)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(1671..2000)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1685..1820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(2011..2757)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2776..3286)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2839..2857)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        3303..3670
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3311..3329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3347..3365
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3383..3401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3419..3437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3455..3473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3491..3509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3527..3545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      3702..3711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4179..4181,4182..4895)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKKHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKVRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4221..4242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4482..4501)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4829..4850
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5075..5209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5253..5368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(5631..5675)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5860..6093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6590..6715
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6762..7395
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7563..7697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7745..7849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7850..7918,7919..8710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8068..8087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8881..9469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9370..9389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9623..9640
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9757..9778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9793..9798,9799..9816,9817..9823)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9831..9847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9836..9858
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9855..9871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9855..9871
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             9999..10632
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(10839..10843,1..584)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
       61 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
      121 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag
      181 gacgtcagct tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
      241 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
      301 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
      361 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
      421 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
      481 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
      541 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgttcca gacgcgtcta
      601 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
      661 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
      721 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
      781 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
      841 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
      901 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
      961 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     1021 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     1081 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     1141 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     1201 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     1261 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
     1321 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
     1381 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     1441 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     1501 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     1561 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggctttcc
     1621 ggatctatcc atctttggta cgggttgtgg caagcttatc atggtcgcgt tttgcaaaag
     1681 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     1741 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     1801 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     1861 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     1921 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     1981 ccacacccta actgacacac attcgcgcgt ttacccgggg agcatgtcaa ggtcaaaatc
     2041 gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag
     2101 catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg
     2161 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg
     2221 ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt
     2281 ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa
     2341 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag
     2401 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg
     2461 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg
     2521 cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag
     2581 catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt
     2641 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac
     2701 tccattgagt aattccagag cagagtttat gactttgctc ttgtccagtc tagacatggt
     2761 aattcgatga tccccctggg gagagaggtc ggtgattcgg tcaacgaggg agccgactgc
     2821 cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc gcgggcagga acagggccca
     2881 cactaccgcc ccacaccccg cctcccgcac cgccccttcc cggccgctgc tctcggcgcg
     2941 ccctgctgag cagccgctat tggccacagc ccatcgcggt cggcgcgctg ccattgctcc
     3001 ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg gcttccgttt gtcacgtccg
     3061 gcacgccgcg aaccgcaagg aaccttcccg acttaggggc ggagcaggaa gcgtcgccgg
     3121 ggggcccaca agggtagcgg cgaagatccg ggtgacgctg cgaacggacg tgaagaatgt
     3181 gcgagaccca gggtcggcgc cgctgcgttt cccggaacca cgcccagagc agccgcgtcc
     3241 ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc aaccccgtgg aattatcacc
     3301 tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     3361 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     3421 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     3481 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     3541 agagaacgta taagctttgc ttatgtaaac cagggcgcct ataaaagagt gctgattttt
     3601 tgagtaaact tcaattccac aacacttttg tcttatacca actttccgta ccacttccta
     3661 ccctcgtaaa gaattcccgt atacaccggt gccggcggat cgccaccatg gagtcgggca
     3721 ctgaggagta tgagctcaac ggcgacctgc gcccgggctc ccccggttcc cccgacgcct
     3781 tgccaccccg ctggggtttg cggaaccggc ccatcaatgt aaaccattat gccaacaaga
     3841 agagcgctgc ggagagcatg ctggacatcg cgctgctcat ggccaacgcg tcgcagctga
     3901 aggccgtggt ggagcagggc aatgatttcg ccttcttcgt gccccttgtg gtcctcatct
     3961 ctatctccct cgtgctgcag ataggagtgg gcgtgctgct catcttcctg gtcaagtatg
     4021 acctcaacaa cccggccaag cacgccaagc tggactttct taacaacctg gccacgggac
     4081 tggttttcat catcgtcgtg gtcaacatct tcattacggc cttcggggtc cagaagcctg
     4141 taatggacgt ggcgccccgg caggggggtg gaggctctgt gagcaagggc gaggagctgt
     4201 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4261 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4321 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4381 tgcagtgctt cagccgctac cccgaccaca tgaagaagca cgacttcttc aagtccgcca
     4441 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4501 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4561 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4621 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggctaac ttcaaggttc
     4681 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4741 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4801 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4861 ggatcactct cggcatggac gagctgtaca agtaagatcc agaccacctc ccctgcgagc
     4921 taagctggac agccaatgac gggtaagaga gtgacatttt tcactaacct aagacaggag
     4981 ggccgtcaga gctactgcct aatccaaaga cgggtaaaag tgataaaaat gtatcactcc
     5041 aacctaagac aggcgcagct tccgagggat ttgagatcca gacatgataa gatacattga
     5101 tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg
     5161 tgatgctatt gccttatttg taaccattat aagctgcaat aaacaagtta agtcgataaa
     5221 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
     5281 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
     5341 accccccaat cccccctttt cttttaaagg cgataccgtc gagatccgtt cactaatcga
     5401 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
     5461 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     5521 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     5581 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     5641 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     5701 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     5761 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     5821 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     5881 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     5941 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     6001 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     6061 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     6121 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     6181 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     6241 agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     6301 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     6361 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     6421 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     6481 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     6541 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     6601 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     6661 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     6721 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     6781 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     6841 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     6901 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     6961 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     7021 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     7081 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     7141 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     7201 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     7261 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     7321 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
     7381 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
     7441 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
     7501 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
     7561 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     7621 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     7681 gctgcaataa acaagttgat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
     7741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8401 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8461 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8701 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8761 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8821 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8881 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8941 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9001 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9061 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9121 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9181 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9241 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9301 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9361 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9421 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9481 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9541 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9601 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9661 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9721 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9781 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9841 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     9901 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     9961 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
    10021 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
    10081 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
    10141 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
    10201 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
    10261 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
    10321 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
    10381 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
    10441 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
    10501 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
    10561 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
    10621 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
    10681 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
    10741 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
    10801 catgtttttc taggtctcga ctgcagaatt aattccaggc ggg
//
