LOCUS       sequence-417110-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses human NINJ1 tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Ramos S, Hartenian E, Santos JC, Walch P, Broz P
  TITLE     NINJ1 induces plasma membrane rupture and release of 
            damage-associated molecular pattern molecules during ferroptosis.
  JOURNAL   EMBO J. 2024 Apr;43(7):1164-1186. doi: 10.1038/s44318-024-00055-y. 
            Epub 2024 Feb 23.
  PUBMED    38396301
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00055-y"; journalName: "EMBO J"; date: 
            "2024-04"; volume: "43"; issue: "7"; pages: "1164-1186"
COMMENT     Sequence Label: pLVX-hNINJ1-GFP
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    168..302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        350..454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(455..523,524..1315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(673..692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1486..2074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1975..1994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2228..2245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2362..2383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2398..2403,2404..2421,2422..2428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2436..2452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2441..2463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2460..2476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2460..2476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2604..3237
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3444..4032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3550..3561
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3959..3979
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4046..5065)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(4046..5065)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(5119..5448)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5133..5268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(5459..6205)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(6224..6734)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6287..6305)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        6751..7118
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6759..6777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6795..6813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6831..6849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6867..6885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6903..6921
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6939..6957
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6975..6993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      7150..7159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7627..7629,7630..8343)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKKHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKVRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7669..7690)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7930..7949)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8277..8298
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8523..8657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8701..8816
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9079..9123)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9308..9541
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10038..10163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10210..10843
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
       61 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
      121 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
      181 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      241 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      301 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      421 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      481 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      541 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      601 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      661 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      721 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      781 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      841 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      901 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      961 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1021 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1081 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1141 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1201 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1261 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1321 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1621 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2161 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2221 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2281 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2341 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2401 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2461 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaacgcta
     2521 gcaatgtcaa ggcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     2581 aataagatga catgaactac tactgctaga gattttccac actgactaaa agggtctgag
     2641 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     2701 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     2761 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     2821 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     2881 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     2941 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     3001 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     3061 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     3121 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     3181 ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc ccttccagtc
     3241 ccctcttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     3301 aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct tctagccagg
     3361 cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt ttttctaggt
     3421 ctcgactgca gaattaattc caggcgggga ggcggcccaa agggagatcc gactcgtctg
     3481 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     3541 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     3601 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     3661 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     3721 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     3781 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     3841 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     3901 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     3961 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     4021 cagaggttga ttgttccaga cgcgtctatt cctttgccct cggacgagtg ctggggcgtc
     4081 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     4141 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     4201 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     4261 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
     4321 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     4381 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     4441 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     4501 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     4561 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     4621 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     4681 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
     4741 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     4801 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
     4861 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     4921 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     4981 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     5041 cagacgtcgc ggtgagttca ggctttccgg atctatccat ctttggtacg ggttgtggca
     5101 agcttatcat ggtcgcgttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5161 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5221 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5281 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5341 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     5401 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tcgcgcgttt
     5461 acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
     5521 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
     5581 tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
     5641 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtttcagaa
     5701 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
     5761 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
     5821 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
     5881 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
     5941 cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
     6001 ggcaggagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
     6061 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
     6121 tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagca gagtttatga
     6181 ctttgctctt gtccagtcta gacatggtaa ttcgatgatc cccctgggga gagaggtcgg
     6241 tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg
     6301 aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg
     6361 ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc
     6421 atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga
     6481 gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac
     6541 ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg
     6601 tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc
     6661 cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc
     6721 gcaaccccaa ccccgtggaa ttatcacctc gagtttactc cctatcagtg atagagaacg
     6781 tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag
     6841 tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt
     6901 actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg
     6961 tatatccagt ttactcccta tcagtgatag agaacgtata agctttgctt atgtaaacca
     7021 gggcgcctat aaaagagtgc tgattttttg agtaaacttc aattccacaa cacttttgtc
     7081 ttataccaac tttccgtacc acttcctacc ctcgtaaaga attcccgtat acaccggtgc
     7141 cggcggatcg ccaccatgga ctcgggaacc gaggagtacg agctcaacgg cggcctgcct
     7201 ccgggcacac ccggctcccc ggacgcctcg ccggcccgct ggggctggag gcacgggccc
     7261 atcaacgtga accattacgc cagcaagaag agcgcagccg agagcatgct ggacatcgcg
     7321 ctgctgatgg ccaacgcgtc ccagctgaag gccgtcgtgg aacagggccc cagcttcgcc
     7381 ttctatgtgc ccctggtggt cctcatctcc atctcccttg tgctgcagat cggcgtgggg
     7441 gtgctgctca tcttccttgt caagtacgac cttaacaacc cggccaagca cgccaagctg
     7501 gacttcctca acaacctggc cacgggcctg gtgttcatca tcgtggtagt caacatcttc
     7561 atcacggcct tcggggtcca gaagcccttg atggacatgg caccccagca ggggggtgga
     7621 ggctctgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7681 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     7741 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     7801 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     7861 aagaagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     7921 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     7981 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     8041 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     8101 aacggcatca aggctaactt caaggttcgc cacaacatcg aggacggcag cgtgcagctc
     8161 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     8221 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     8281 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     8341 taagatccag accacctccc ctgcgagcta agctggacag ccaatgacgg gtaagagagt
     8401 gacatttttc actaacctaa gacaggaggg ccgtcagagc tactgcctaa tccaaagacg
     8461 ggtaaaagtg ataaaaatgt atcactccaa cctaagacag gcgcagcttc cgagggattt
     8521 gagatccaga catgataaga tacattgatg agtttggaca aaccaaaact agaatgcagt
     8581 gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta accattataa
     8641 gctgcaataa acaagttaag tcgataaact ggatctctgc tgtccctgta ataaacccga
     8701 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
     8761 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaggcg
     8821 ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
     8881 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
     8941 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     9001 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     9061 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     9121 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     9181 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     9241 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     9301 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     9361 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     9421 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     9481 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     9541 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
     9601 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
     9661 catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc ggtggtctta
     9721 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     9781 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     9841 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     9901 aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     9961 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
    10021 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
    10081 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
    10141 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
    10201 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
    10261 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
    10321 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
    10381 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
    10441 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
    10501 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
    10561 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
    10621 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
    10681 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
    10741 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
    10801 tcaaggatat cttgtcttct ttgggagtga attagccctt cca
//
