LOCUS       sequence-417137-        6088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ark313 rep/cap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, 
            Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, 
            Havlik LP, Pimentel H, Asokan A, Eyquem J
  TITLE     An evolved AAV variant enables efficient genetic engineering of 
            murine T cells.
  JOURNAL   Cell. 2023 Jan 19;186(2):446-460.e19. doi: 
            10.1016/j.cell.2022.12.022. Epub 2023 Jan 12.
  PUBMED    36638795
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.022"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "446-460.e19"
COMMENT     Sequence Label: pArk313
FEATURES             Location/Qualifiers
     source          1..6088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4077..4088
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      complement(4339..4927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4419..4438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5098..5889,5890..5958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5721..5740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5959..6063)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatatcctta aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
       61 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
      121 gaggtttgaa cgcgcagcca ccacggcggg gttttacgag attgtgatta aggtccccag
      181 cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa
      241 ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct
      301 gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc
      361 cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt
      421 gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg
      481 cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc
      541 ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat
      601 ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga
      661 acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct
      721 gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc
      781 gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga
      841 caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt
      901 caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat
      961 tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat
     1021 ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc
     1081 ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt
     1141 tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt
     1201 ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat
     1261 ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat
     1321 tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc
     1381 gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac
     1441 gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg
     1501 tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc
     1561 aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa
     1621 aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc
     1681 gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa
     1741 atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat
     1801 gaatcagaat tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc
     1861 cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat
     1921 tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtgga
     1981 tttggatgac tgcatctttg aacaataaat gatttaaatc aggtatggct gccgatggtt
     2041 atcttccaga ttggctcgag gacaacctct ctgagggcat tcgcgagtgg tgggacttga
     2101 aacctggagc cccgaaaccc aaagccaacc agcaaaagca ggacgacggc cggggtctgg
     2161 tgcttcctgg ctacaagtac ctcggaccct tcaacggact cgacaagggg gagcccgtca
     2221 acgcggcgga tgcagcggcc ctcgagcacg acaaggccta cgaccagcag ctcaaagcgg
     2281 gtgacaatcc gtacctgcgg tataaccacg ccgacgccga gtttcaggag cgtctgcaag
     2341 aagatacgtc ttttgggggc aacctcgggc gagcagtctt ccaggccaag aagagggttc
     2401 tcgaaccttt tggtctggtt gaggaaggtg ctaagacggc tcctggaaag aaacgtccgg
     2461 tagagcagtc gccacaagag ccagactcct cctcgggcat tggcaagaca ggccagcagc
     2521 ccgctaaaaa gagactcaat tttggtcaga ctggcgactc agagtcagtc cccgacccac
     2581 aacctctcgg agaacctcca gcaacccccg ctgctgtggg acctactaca atggcttcag
     2641 gcggtggcgc accaatggca gacaataacg aaggcgccga cggagtgggt aatgcctcag
     2701 gaaattggca ttgcgattcc acatggctgg gcgacagagt catcaccacc agcacccgaa
     2761 catgggcctt gcccacctat aacaaccacc tctacaagca aatctccagt gcttcaacgg
     2821 gggccagcaa cgacaaccac tacttcggct acagcacccc ctgggggtat tttgatttca
     2881 acagattcca ctgccatttc tcaccacgtg actggcagcg actcatcaac aacaattggg
     2941 gattccggcc caagagactc aacttcaagc tcttcaacat ccaagtcaag gaggtcacga
     3001 cgaatgatgg cgtcacgacc atcgctaata accttaccag cacggttcaa gtcttctcgg
     3061 actcggagta ccagttgccg tacgtcctcg gctctgcgca ccagggctgc ctccctccgt
     3121 tcccggcgga cgtgttcatg attccgcagt acggctacct aacgctcaac aatggcagcc
     3181 aggcagtggg acggtcatcc ttttactgcc tggaatattt cccatcgcag atgctgagaa
     3241 cgggcaataa ctttaccttc agctacacct tcgaggacgt gcctttccac agcagctacg
     3301 cgcacagcca gagcctggac cggctgatga atcctctcat cgaccagtac ctgtattacc
     3361 tgaacagaac tcagaatcag tccgtggtca acccggccga gggcttgctg tttagccggg
     3421 ggtctccagc tggcatgtct gttcagccca aaaactggct acctggaccc tgttaccggc
     3481 agcagcgcgt ttctaaaaca aaaacagaca acaacaacag caactttacc tggactggtg
     3541 cttcaaaata taaccttaat gggcgtgaat ctataatcaa ccctggcact gctatggcct
     3601 cacacaaaga cgacaaagac aagttctttc ccatgagcgg tgtcatgatt tttggaaagg
     3661 agagcgccgg agcttcaaac actgcattgg acaatgtcat gatcacagac gaagaggaaa
     3721 tcaaagccac taaccccgtg gccaccgaaa gatttgggac tgtggcagtc aatctccaga
     3781 gcagcagcac agaccctgcg accggagatg tgcatgttat gggagcctta cctggaatgg
     3841 tgtggcaaga cagagacgta tacctgcagg gtcctatttg ggccaaaatt cctcacacgg
     3901 atggacactt tcacccgtct cctctcatgg gcggctttgg actgaagcac ccgcctcctc
     3961 agatcctcat caaaaacacg cctgttcctg cgaatcctcc ggcagagttt tcggctacaa
     4021 agtttgcttc attcatcacc cagtattcca caggacaagt gagcgtggag attgaatggg
     4081 agctgcagaa agaaaacagc aaacgctgga atcccgaagt gcagtataca tctaactatg
     4141 caaaatctgc caacgttgat ttcactgtgg acaacaatgg actttatact gagcctcgcc
     4201 ccattggcac ccgttacctc acccgtcccc tgtaattgtc tgttaatcaa taaaccggtt
     4261 gattcgtttc agttgaactt tggtctagag atatcagcaa aaggccagga accgtaaaaa
     4321 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4381 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4561 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4681 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4741 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4801 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5161 tgcctggctc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6061 gcgcacattt ccccgaaaag tgccacct
//
