LOCUS       sequence-417143-       11516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene expression in B. dendrobatidis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK
  TITLE     Genetic transformation of the frog-killing chytrid fungus 
            Batrachochytrium dendrobatidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jan 23;121(4):e2317928121. doi: 
            10.1073/pnas.2317928121. Epub 2024 Jan 18.
  PUBMED    38236738
REFERENCE   2  (bases 1 to 11516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-01-23"; volume: "121(4):e2317928121. 
            doi"; pages: " 10.1073/pnas.2317928121. Epub 2024 Jan 18"
COMMENT     Sequence Label: pEK19
FEATURES             Location/Qualifiers
     source          1..11516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     206..228
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     220..237
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     221..237
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             619..1644
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             619..1644
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1660..2175
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     terminator      2197..2384
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2252..2276)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2399..2415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2399..2415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2412..2434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2423..2439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2447..2453,2454..2471,2472..2477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2492..2513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2690..2714
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(2996..3015)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3007..3822
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3007..3822
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3080..3099)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3917..4505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4406..4425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4659..4676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4691..4831
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      5175..5369
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5435..6508)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6937..7566)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      8727..10069
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9690..9737)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10110..10128
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10331..10546
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10547..11350
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      121 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      181 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      241 aagcttgtac cttgtagctt atactgatgg caaagaatgc agtcagatac taacagtttg
      301 tattagggaa caaacgggca aggggactga ggttaagaag ttggctccag agccactggc
      361 accgccagaa ccatcaaaat aggactcaat acattgattc agaattgaaa cagaatgttt
      421 ttttaaatgg caggaatgcc ttggagagag attgttcaac tgatagcatg ttgaagagtt
      481 gaaacaaaca agcacagtca cgtgtaatcc agccaatcaa tcgctgacac cggttctaag
      541 ttggaaaata agcgacattc gactataaaa agcgatgatc agatctgtct gcacacccac
      601 actgcatcat cttaattaat gggtaaaaag cctgaactca ccgcgacgtc tgtcgagaag
      661 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
      721 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
      781 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
      841 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
      901 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
      961 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     1021 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     1081 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     1141 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     1201 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     1261 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     1321 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     1381 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     1441 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     1501 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     1561 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     1621 agcactcgtc cgagggcaaa ggaaggagga ggaggatcca tggtcttcac actcgaagat
     1681 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
     1741 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
     1801 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
     1861 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
     1921 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
     1981 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
     2041 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
     2101 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
     2161 cgcattctgg cgtaaaggcg cgccacttct aaataagcga atttcttatg atttatgatt
     2221 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     2281 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     2341 caggtatagt atgaggtcgc tcttattgac cacacctcta ccgggaattc gtaatcatgt
     2401 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2461 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2521 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2581 gccaacgcgc ggggagaggc ggtttgcgta ttggctagag caattcggcg ttaattcagt
     2641 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     2701 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     2761 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     2821 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     2881 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     2941 tgcctgtgat ccagatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     3001 ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac
     3061 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     3121 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     3181 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3241 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3301 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3361 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3421 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3481 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3541 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3601 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3661 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3721 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3781 aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat tcatgatcct
     3841 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4681 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4741 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     4801 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     4861 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4921 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     4981 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     5041 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     5101 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     5161 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     5221 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     5281 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     5341 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     5401 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     5461 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     5521 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     5581 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     5641 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     5701 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     5761 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     5821 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     5881 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     5941 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     6001 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     6061 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     6121 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     6181 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     6241 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     6301 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     6361 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     6421 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     6481 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     6541 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     6601 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     6661 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     6721 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     6781 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     6841 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     6901 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     6961 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     7021 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     7081 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     7141 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     7201 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     7261 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     7321 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     7381 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     7441 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     7501 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     7561 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     7621 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     7681 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     7741 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
     7801 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     7861 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     7921 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     7981 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     8041 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     8101 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     8161 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     8221 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     8281 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     8341 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     8401 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     8461 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     8521 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     8581 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     8641 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
     8701 aacgtcagaa ggaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa
     8761 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     8821 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa
     8881 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     8941 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt
     9001 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt
     9061 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg
     9121 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact
     9181 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg
     9241 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat
     9301 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta
     9361 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
     9421 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
     9481 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
     9541 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
     9601 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
     9661 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac
     9721 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     9781 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     9841 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     9901 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     9961 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
    10021 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa
    10081 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10141 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10201 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10261 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10321 ataccacctt ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg
    10381 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
    10441 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
    10501 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac
    10561 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
    10621 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
    10681 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
    10741 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
    10801 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
    10861 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
    10921 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
    10981 aggccttttg atgctagcag aattgtcatg caagggctcc ctatctactg gagaatatac
    11041 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
    11101 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
    11161 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc
    11221 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
    11281 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
    11341 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
    11401 ttcaatttaa ttatatcagt tattacctgg catgcacata caaatggacg aacggataaa
    11461 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttag
//
