LOCUS       sequence-417146-       11630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene expression in B. dendrobatidis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK
  TITLE     Genetic transformation of the frog-killing chytrid fungus 
            Batrachochytrium dendrobatidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jan 23;121(4):e2317928121. doi: 
            10.1073/pnas.2317928121. Epub 2024 Jan 18.
  PUBMED    38236738
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-01-23"; volume: "121(4):e2317928121. 
            doi"; pages: " 10.1073/pnas.2317928121. Epub 2024 Jan 18"
COMMENT     Sequence Label: pEK39
FEATURES             Location/Qualifiers
     source          1..11630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     206..228
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     220..237
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     221..237
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             536..1561
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             536..1561
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2311..2498
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2366..2390)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2513..2529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2513..2529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2526..2548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2537..2553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2561..2567,2568..2585,2586..2591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2606..2627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2804..2828
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(3110..3129)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3121..3936
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3121..3936
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3194..3213)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4031..4619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4520..4539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4773..4790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4805..4945
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      5289..5483
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5549..6622)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7051..7680)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      8841..10183
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9804..9851)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10224..10242
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10445..10660
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10661..11464
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      121 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      181 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      241 aagcttttat gctccaagcg gagacgatga ggccgatatt acagtggctt agtagcgtgt
      301 acaatatttc cataaaaggc tacatggtga gtttttcacg tgaccgtaat tagtcccaat
      361 taggctttat tgtatattgc tactaagcat cgttaaatga cctgctcgaa gcttcgtcta
      421 taaaaggcgg gcgtgtgtgc gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact
      481 tgaccaagac ctttcacccg caccactctc atccatcaca cacacaaatt aattaatggg
      541 taaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
      601 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
      661 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
      721 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
      781 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
      841 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
      901 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
      961 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     1021 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     1081 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     1141 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     1201 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     1261 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     1321 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     1381 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     1441 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     1501 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     1561 aggaggagga ggatccatgg tgtctaaagg agaggaactg attaaggaaa acatgagaat
     1621 gaaggtggtg atggaaggct ctgtgaatgg ccaccagttc aaatgtacag gggaggggga
     1681 aggaaggcct tacgagggcg tgcagaccat gagaatcaag gtgattgagg ggggcccact
     1741 gccctttgcc ttcgacattc tggccacttc tttcatgtat ggctccagga cctttatcaa
     1801 gtaccctgcc gacatccctg actttttcaa acagagcttt cccgagggat tcacatggga
     1861 gcgggtgact cggtatgagg atggaggggt ggtgaccgtg actcaggata ctagcctgga
     1921 ggacggcgaa ctggtgtaca acgtgaaggt gagaggcgtg aactttccat ctaatggacc
     1981 tgtgatgcag aagaaaacca aaggatggga gcctaatact gagatgatgt atcccgccga
     2041 tggcggcctg agaggctaca ccgatatcgc cctgaaggtg gatggaggag gccacctgca
     2101 ctgcaatttc gtgacaacct atagaagcaa aaagacagtg gggaacatta aaatgcccgg
     2161 cgtgcatgcc gtggatcaca ggctggaaag gatcgaagaa tccgacaatg aaacatacgt
     2221 ggtgcagagg gaagtggccg tggccaaata cagcaatctg gggggcggaa tggacgagct
     2281 gtacaaataa ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
     2341 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     2401 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     2461 tagtatgagg tcgctcttat tgaccacacc tctaccggga attcgtaatc atgtcatagc
     2521 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2581 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2641 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2701 gcgcggggag aggcggtttg cgtattggct agagcaattc ggcgttaatt cagtacatta
     2761 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     2821 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     2881 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     2941 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     3001 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     3061 tgatccagat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     3121 atgagccata ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat
     3181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     3241 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     3301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     3361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     3421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     3481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     3541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     3601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     3661 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     3721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     3781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3901 ttgcagtttc atttgatgct cgatgagttt ttctaagaat taattcatga tcctgcatga
     3961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4141 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4741 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4801 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     4861 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     4921 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     4981 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5041 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     5101 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     5161 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     5221 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     5281 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     5341 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     5401 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     5461 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     5521 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     5581 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     5641 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     5701 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     5761 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     5821 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     5881 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     5941 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     6001 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     6061 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     6121 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     6181 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     6241 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     6301 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     6361 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     6421 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     6481 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     6541 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     6601 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     6661 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     6721 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     6781 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     6841 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     6901 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     6961 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     7021 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     7081 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     7141 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     7201 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     7261 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     7321 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     7381 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     7441 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
     7501 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
     7561 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
     7621 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
     7681 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
     7741 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
     7801 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
     7861 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
     7921 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
     7981 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
     8041 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
     8101 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
     8161 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
     8221 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
     8281 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
     8341 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
     8401 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
     8461 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
     8521 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
     8581 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
     8641 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
     8701 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
     8761 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
     8821 agaaggaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     8881 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     8941 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
     9001 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     9061 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
     9121 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
     9181 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
     9241 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
     9301 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
     9361 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
     9421 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
     9481 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
     9541 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
     9601 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
     9661 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
     9721 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
     9781 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
     9841 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     9901 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     9961 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
    10021 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
    10081 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
    10141 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatactaa gaaaccatta
    10201 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10261 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10321 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10381 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatacca
    10441 ccttttcaat tcatcatttt ttttttattc ttttttttga tttcggtttc cttgaaattt
    10501 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
    10561 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
    10621 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
    10681 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
    10741 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
    10801 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
    10861 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
    10921 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
    10981 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
    11041 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
    11101 tttgatgcta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
    11161 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
    11221 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
    11281 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
    11341 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
    11401 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
    11461 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
    11521 ttaattatat cagttattac ctggcatgca catacaaatg gacgaacgga taaacctttt
    11581 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
//
