LOCUS       sequence-417149-       10294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter gene expression in B. dendrobatidis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK
  TITLE     Genetic transformation of the frog-killing chytrid fungus 
            Batrachochytrium dendrobatidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jan 23;121(4):e2317928121. doi: 
            10.1073/pnas.2317928121. Epub 2024 Jan 18.
  PUBMED    38236738
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-01-23"; volume: "121(4):e2317928121. 
            doi"; pages: " 10.1073/pnas.2317928121. Epub 2024 Jan 18"
COMMENT     Sequence Label: pEM112B
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     91..113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     105..122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     106..122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(316..1029,1030..1032,1033..1035))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(361..382)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     969..990
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             1467..1523
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1533..1586
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1605..2633
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1605..2633
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(2726..2742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2726..2742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2817..2841
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3265..4059
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5345..5933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5834..5853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6087..6104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6119..6259
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6345..6367
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6603..6797
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6863..7936)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQTGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8365..8994)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca cccagtcacg acgttgtaaa acgacggcca
      121 gtgccagatc taaacaaaaa cgttacttca atggggaaac aaaggccaaa tcttcaatat
      181 ttaaaccaag gtgcaccttt acaaaaatat ctacactaaa aaagtagagc tgaaaagctc
      241 tgatttcgtt ttttaaaatc aaagatcggt cttgaccgat gaagggtgta tgcgtgtgta
      301 agtaggtgtg gtacctcact tgtacagctc gtccatgcca tgtgtaatcc cggcggcggt
      361 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga
      421 ctgatggctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      481 ctgctggtag tggtcggcga gctgcacgct gccgtcctca acgttgtggc ggatcttgaa
      541 gttagccttg atgcagttct tctgcttgtc ggccgtgata tagacgtagt ggctgttgaa
      601 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      661 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtaggt
      721 accgtcgtcc ttgaaagaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
      781 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcaggcca cgccgtagcc
      841 gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      901 cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg ccgcggacgc tgaacttgtg
      961 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     1021 gcccttgctc accattctag atgctgtgta aggtgtgcgt gagagagagg gtcgtgagtg
     1081 agtcttgaag agaaacgtca aagcttgaag cgtagcagaa tacggaaaac gacttggggg
     1141 aatggcgagg agcgtcgccc ctaggtttta tgctccaagc ggagacgatg aggccgatat
     1201 tacagtggct tagtagcgtg tacaatattt ccataaaagg ctacatggtg agtttttcac
     1261 gtgaccgtaa ttagtcccaa ttaggcttta ttgtatattg ctactaagca tcgttaaatg
     1321 acctgctcga agcttcgtct ataaaaggcg ggcgtgtgtg cgcttccccc gctacgtctt
     1381 tttcacaaac actttgcgac ttgaccaaga cctttcaccc gcaccactct catccatcac
     1441 acacacaagt cgacatggga agcggagcta ctaacttcag cctgctgaag caggctggag
     1501 acgtggagga gaaccctgga cctggaagcg gagagggcag aggaagtctg ctaacatgcg
     1561 gtgacgtcga ggagaatcct ggacctaccg agctcggatc taccatgggt aaaaagcctg
     1621 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     1681 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     1741 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     1801 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     1861 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     1921 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     1981 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     2041 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     2101 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     2161 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     2221 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     2281 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     2341 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     2401 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     2461 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     2521 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     2581 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taatggcgcg
     2641 cctatataaa ctcatttact tatgtaggaa taaagagtat catctttcaa agagctcgat
     2701 atcttaatta agaattcgta atcatgtcat agctgtttcc tgtgtgaaaa ttcggcgtta
     2761 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     2821 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     2881 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     2941 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     3001 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     3061 gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg
     3121 ccaactttga aaacaacttt gaaaagctgt tttctggtat ttaaggtttt agaatgcaag
     3181 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
     3241 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     3301 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     3361 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     3421 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     3481 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     3541 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     3601 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     3661 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     3721 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     3781 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     3841 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     3901 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     3961 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     4021 aaaataaaat attatatttt actggatgaa ttgttttagt acctagggaa ggtgcgaata
     4081 agcggggaaa ttcttctcgg ctgactcagt catttcattt cttcatgttt gagccgattt
     4141 tttctcccgt aaatgccttg aatcagccta tttagaccgt ttcttcgcca tttaaggcgt
     4201 tatccccagt ttttagtgag atctctccca ctgacgtatc atttggtccg cccgaaacag
     4261 gttggccagc gtgaataaca tcgccagttg gttatcgttt ttcagcaacc ccttgtatct
     4321 ggctttcacg aagccgaact gtcgcttgat gatgcgaaat gggtgctcca ccctggcccg
     4381 gatgctggct ttcatgtatt cgatgttgat ggccgttttg ttcttgcgtg gatgctgttt
     4441 caaggttctt accttgccgg ggcgctcggc gatcagccag tccacatcca cctcggccag
     4501 ctcctcgcgc tgtggcgccc cttggtagcc ggcatcggct gagacaaatt gctcctctcc
     4561 atgcagcaga ttacccagct gattgaggtc atgctcgttg gccgcggtgg tgaccaggct
     4621 gtgggtcagg ccactcttgg catcgacacc aatgtgggcc ttcatgccaa agtgccactg
     4681 attgcctttc ttggtctgat gcatctccgg atcgcgttgc tgctctttgt tcttggtcga
     4741 gctgggtgcc tcaatgatgg tggcatcgac caaggtgcct tgagtcatca tgacgcctgc
     4801 ttcggccagc cagcgattga tggtcttgaa caattggcgg gccagttgat gctgctccag
     4861 caggtggcgg aaattcatga tggtggtgcg gtccggcaag gcgctatcca gggataaccg
     4921 ggcaaacaga cgcatggagg cgatttcgta cagagcatct tccatcgcgc catcgctcag
     4981 gttgtaccaa tgctgcatgc agtgaatgcg tagcatggtt tccagcggat aaggtcgccg
     5041 gccattacca gccttggggt aaaacggctc gatgacttcc accatgtttt gccatggcag
     5101 aatctgctcc atgcgggaca agaaaatctc ttttctggtc tgacggcgct tactgctgaa
     5161 ttcactgtcg gcgaaggtaa gttgatgact catgatgaac cctgttctat ggctccagat
     5221 gacaaacatg atctcatatc agggacttgt tcgcaccttc cctagaatgc atgaccaaaa
     5281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5461 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggctt cgccacctct
     5881 gacttgagcg tcgacttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6121 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     6181 tcagtacaat ctgctctgac gccgcatagt taagccagta tacactccgc tatcgctacg
     6241 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     6301 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     6361 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     6421 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     6481 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     6541 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     6601 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     6661 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     6721 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     6781 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     6841 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     6901 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     6961 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     7021 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     7081 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     7141 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     7201 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     7261 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     7321 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     7381 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     7441 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     7501 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     7561 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     7621 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     7681 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     7741 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     7801 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     7861 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc ggtttgtacc gggccggatg
     7921 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     7981 gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     8041 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     8101 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     8161 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     8221 ccgccggcaa ctgaaagttg acccgcttca cggctggcgt gtctgccagg ctggccaacg
     8281 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     8341 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     8401 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     8461 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     8521 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     8581 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     8641 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     8701 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     8761 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     8821 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     8881 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     8941 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     9001 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     9061 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     9121 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     9181 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
     9241 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
     9301 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
     9361 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
     9421 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
     9481 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
     9541 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
     9601 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
     9661 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
     9721 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
     9781 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
     9841 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
     9901 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
     9961 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    10021 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    10081 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    10141 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    10201 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
    10261 tccgattatt ctaataaacg ctcttttctc ttag
//
