LOCUS       sequence-417184-       13222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector has been generated for introduction of SGFP in 
            filamentous fungi using ATMT. Contains the hygromycin resistance 
            gene and the SGFP gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Sesma A, Osbourn AE
  TITLE     The rice leaf blast pathogen undergoes developmental processes 
            typical of root-infecting fungi.
  JOURNAL   Nature. 2004 Sep 30;431(7008):582-6. doi: 10.1038/nature02880.
  PUBMED    15457264
REFERENCE   2  (bases 1 to 13222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2004-09-30- 30"; volume: "431"; issue: "7008"; 
            pages: "582-6"
COMMENT     Sequence Label: pCAMBgfp
FEATURES             Location/Qualifiers
     source          1..13222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      584..1172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1073..1092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1326..1343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1358..1498
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1424..1443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1584..1606
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1842..2036
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2102..3175)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3604..4233)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5534..5558
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5746..5768
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5760..5777
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5761..5777
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5781..5837
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      complement(5861..6113)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(6132..6845,6846..6848,6849..6851))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(6177..6198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6526..6545
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6785..6806
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7638..8663
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8704..8878
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8956..8980
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9405..10199
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
       61 ccgtgtagtg cgactgaagc tcgcaatacc ataagccgtt tttgctcacg gatatcagac
      121 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
      181 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
      241 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
      301 gttttacagt caagtaaggc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
      361 aattcggggc agaattggta aagagagtcg tgtaaaatat cgagttcgca cattttgttg
      421 tctgattatt gatttttggc gaaaccattt gatcatatga caagatgtgt atctacctta
      481 acttaatgat tttgataaaa atcattaggg gattcatcag cccttaacgt gagttttcgt
      541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      721 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     1381 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     1501 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1561 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1621 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg
     1681 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag
     1741 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag
     1801 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg
     1861 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt
     1921 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt
     1981 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa
     2041 agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac
     2101 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg
     2161 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca
     2221 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga
     2281 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga
     2341 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg
     2401 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact
     2461 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg
     2521 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg
     2581 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc
     2641 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct
     2701 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt
     2761 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg
     2821 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg
     2881 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa
     2941 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg
     3001 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
     3061 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
     3121 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
     3181 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg caggcaaccc agccgcttac
     3241 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
     3301 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
     3361 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
     3421 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
     3481 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
     3541 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
     3601 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
     3661 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
     3721 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
     3781 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
     3841 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
     3901 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
     3961 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
     4021 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
     4081 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
     4141 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
     4201 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
     4261 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
     4321 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
     4381 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
     4441 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
     4501 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
     4561 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
     4621 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
     4681 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
     4741 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
     4801 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
     4861 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
     4921 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
     4981 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
     5041 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
     5101 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
     5161 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
     5221 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
     5281 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
     5341 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
     5401 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
     5461 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
     5521 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     5581 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     5641 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     5701 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     5761 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg
     5821 gtaccgagct cgaattctca tgtttgacag cttatcatcg gatctagtaa catagatgac
     5881 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     5941 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     6001 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     6061 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcagcc
     6121 gggcggccgc tttacttgta cagctcgtcc atgccgtgag tgatcccggc ggcggtcacg
     6181 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     6241 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     6301 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     6361 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     6421 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     6481 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     6541 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     6601 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtgaag
     6661 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     6721 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     6781 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     6841 ttgctcacca tggcctatat tcattcattg tcagctatcg ctggcaacaa gatataagcg
     6901 tgtacttacg attctaggac cctgcgaata gagttggagt tggataccga tggacgattg
     6961 ttagatagga cgggatgctg atagtcgagg taagttgttg agggtggtgg ttttatcaag
     7021 gacggacacc ttagcccttt atatacacag aatcttattg ctaggtagcc ggacggtgcg
     7081 gattccaggt atcagtaggc cctctgaatg tccatgcaat gatgcccaac aatcgagacg
     7141 atcctacttc ggaaccgact cgatcctcgt cgggcctaca tgtatgagac catgtgcacc
     7201 tccgaagcat ccttacggag agcggaattg cgcgtacaga actcctccat gcattccaat
     7261 cgataccgtc gacgttaact gatattgaag gagcattttt tgggcttggc tggagctagt
     7321 ggaggtcaac aatgaatgcc tattttggtt tagtcgtcca ggcggtgagc acaaaatttg
     7381 tgtcgtttga caagatggtt catttaggca actggtcaga tcagccccac ttgtagcagt
     7441 agcggcggcg ctcgaagtgt gactcttatt agcagacagg aacgaggaca ttattatcat
     7501 ctgctgcttg gtgcacgata acttggtgcg tttgtcaagc aaggtaagtg gacgacccgg
     7561 tcataccttc ttaagttcgc ccttcctccc tttatttcag attcaatctg acttacctat
     7621 tctacccaag catccaaatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     7681 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     7741 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     7801 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     7861 cggaagtgct tgacattggg gagttcagcg agagcctgac ctattgcatc tcccgccgtg
     7921 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctccagccgg
     7981 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     8041 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     8101 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     8161 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     8221 tgcatgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     8281 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcctcttct
     8341 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     8401 agcttgcagg atcgccgcgc ctccgggcgt atatgctccg cattggtctt gaccaactct
     8461 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     8521 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     8581 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     8641 ctcgtccgag ggcaaaggaa tagagtagat gccgaccggg aaccagttaa cgtcgacctc
     8701 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     8761 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     8821 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
     8881 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     8941 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     9001 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     9061 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     9121 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     9181 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
     9241 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     9301 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     9361 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     9421 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     9481 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     9541 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     9601 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     9661 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     9721 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     9781 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     9841 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     9901 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     9961 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    10021 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    10081 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    10141 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
    10201 acctagaatg catgaccaaa atcccttaac gctgagagat cccctcataa tttccccaaa
    10261 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
    10321 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
    10381 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
    10441 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
    10501 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
    10561 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
    10621 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
    10681 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
    10741 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
    10801 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
    10861 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
    10921 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
    10981 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
    11041 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
    11101 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
    11161 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
    11221 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
    11281 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
    11341 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
    11401 aattcggggc agaattggta aagagagtcg tgtaaaatat cgagttcgca catcttgttg
    11461 tctgattatt gatttttcgc gaaaccattt gatcatatga caagatgtgt atccacctta
    11521 acttaatgat ttttaccaaa atcattaggg gattcatcag ggcgttgtat gacgtggaag
    11581 cctggaccga tatgttcccg gaatttggtg gcgattcctc ggcgcagacc gacaacttta
    11641 tgaccaaacg cgccagcggt ctggcgacgt atcggaacac cgacttcttc ggcgttatcg
    11701 atggcctgaa cttaaccctg caatatcaag ggaaaaacga aaaccgcgac gttaaaaagc
    11761 aaaacggcga tggcttcggc acgtcattga catatgactt tggcggcagc gatttcgcca
    11821 ttagtggggc ctataccaac tcagatcgca ccaacgagca gaacctgcaa agccgtggca
    11881 caggcaagcg tgcagaagca tgggcaacag gtctgaaata cgatgccaat aatatttatc
    11941 tggcaacttt ctattctgaa acacgcaaaa tgacgccaat aactggcggc tttgccaata
    12001 agacacagaa ctttgaagcg gtcgctcaat accagtttga ctttggtctg cgtccatcgc
    12061 tgggttatgt cttatcgaaa gggaaagata ttgaaggtat cggtgatgaa gatctggtca
    12121 attatatcga cgtcggtgct acgtattatt tcaacaaaaa tatgtcagcg tttgttgatt
    12181 ataaaatcaa ccaactggat agcgataaca aattgaatat taataatgat gatattgtcg
    12241 cggttggcat gacgtatcag ttttaatgaa tattgccgga tgtgatgcat ccggcacatt
    12301 tcactcacgc cgttaacttc accggctcgt cacgaaaatc atccgccggt tccagcttca
    12361 gattcagcgt cgtcagcagt tcacgcagct tctcgtgaaa ctcacgcagg gtgctgagag
    12421 atcccctcat aatttcccca aaacgtaacc atgtgtgaat agattttgag taagcagggt
    12481 tgcagccacg agtgagtctt cccttgttat tgtgtagcca gaatgccgca aaacttccat
    12541 gcctaagcga actgttgaga gtacgtttcg atttctgact gtgttagcct ggaagtgctt
    12601 gtcccaacct tgtttctgag catgaacgcc cgcaagccaa catgttagtt gaagcatcag
    12661 ggcgattagc agcatgatat caaaacgctc tgagctgctc gttcggctat ggcgtaggcc
    12721 tagtccgtag gcaggacttt tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga
    12781 atagatatta acaagttgtt tgggtgttcg aatttcaaca ggtaagttag ttgctagaat
    12841 ccatggctcc tttgccgacg ctgagtagat tttaggtgac gggtggtgac aatgagtccg
    12901 tgtcgagcgc tgattttttc ggcctttaga gcgagattta tacaatagaa tttggcatga
    12961 gattgggttg cttttagtca gcctcttata gcctaaagtc tttgagtgac tagatgacat
    13021 atcatgtaag ttgctgatag gtttccagtt ttccgctcct aggtctgcat attgtacttt
    13081 tcctcttact cgacttaacc agtaccaacc cagcttctca acggatttat accatggcac
    13141 tttaaagcca gcatcactga caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag
    13201 gtcggctaga aattggtcat ga
//
