LOCUS       sequence-417204-       13321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector encoding FIP200 mutant-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
  TITLE     Structure and activation of the human autophagy-initiating 
            ULK1C:PI3KC3-C1 supercomplex
  JOURNAL   bioRxiv 2023.06.01.543278
REFERENCE   2  (bases 1 to 13321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.01.543278"
COMMENT     Sequence Label: 
            pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro
FEATURES             Location/Qualifiers
     source          1..13321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    7081..7133
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      7141..7150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7147..7857
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7292..7310)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7560..7579
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7911..7931)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7916..8245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8096..8231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8319..9344
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8319..9344
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    9400..9988
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9453..9473)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9871..9882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10062..10295
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10367..10488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10404..10423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10458..10477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10528..10663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10696..10712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10696..10712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10709..10731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10720..10736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10744..10750,10751..10768,10769..10774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10789..10810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10927..10944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11098..11686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(11857..12648,12649..12717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12480..12499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12718..12822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12890..12908
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12946..12968)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13068..13087
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13281..13303
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13295..13312
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13296..13312
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgccaccat gaagctctat gtgtttctcg tgaataccgg cactaccctg
     2341 acctttgata ccgaactgac cgtccagacc gtggctgacc tgaagcatgc tatccagtct
     2401 aaatacaaga tcgcaattca gcaccaggac ctggtggtca acggcggaga gtgcatggcc
     2461 gctgatcgca gggtctgtac ctattcagcc ggcaccgaca ctaacccaat cttcctcttt
     2521 aataaggaga tgattctgtg cgatagaccc cctgctatcc ccaagaccac ttttagcact
     2581 gagaacgaca tggaaatcaa agtggaggaa tccctgatga tgccagccgt gttccacacc
     2641 gtcgcttctc gcactcagct ggcactcgag atgtacgaag tggccaagaa actgtgctct
     2701 ttctgtgagg gtctcgtcca cgatgaacat ctgcagcacc agggctgggc agccattatg
     2761 gctaacctgg aggactgttc aaatagttat cagaaactgc tcttcaagtt tgagtctatc
     2821 tactcaaact atctgcagag catcgaagac attaaactga agctcacaca cctgggaacc
     2881 gcagtgagcg tcatggccaa gatccctctg ctcgagtgcc tgactaggca ttcctacaga
     2941 gaatgtctgg gtaggctcga tagtctgcca gaacacgagg acagcgagaa agccgaaatg
     3001 aagcgctcta cagagctggt gctctcacct gacatgccaa ggacaaccaa cgaaagtctg
     3061 ctcaccagct tcccaaagtc cgtggagcat gtctcccccg acactgccga tgctgaatct
     3121 ggaaaggaga ttagagaaag ttgccagagc accgtgcacc agcaggacga gactacaatt
     3181 gacactaagg acggcgatct gcccttcttt aacgtcagtc tgctcgattg gatcaacgac
     3241 caggacagac ctaatgatgt ggagtccgac gtccggaagt gctttgatag catgtcccgt
     3301 ctggacccac gtatcattcg tcctttcatc gcagagtgtc gtcagaccat tgccaagctg
     3361 gacaaccaga atatgaaagc tattaagggt ctggaagatc ggctctacgc cctggaccag
     3421 atgatcgcta gctgtggacg cctggtgaac gagcagaagg aactcgctca gggtttcctg
     3481 gcaaaccaga aaagagccga gaatctcaag gatgcatcag tgctgcctga cctgtgcctc
     3541 agtcatgcca atcagctgat gattatgctc cagaaccacc gcaagctgct cgacatcaaa
     3601 cagaagtgta ccactgctaa acaggagctg gcaaacaatc tccatgtgag gctgaagtgg
     3661 tgctgtttcg tcatgctgca cgcagaccag gatggcgaga agctccaggc cctgctcagg
     3721 ctggtgattg agctgctcga aagagtgaag atcgtcgagg ctctgtccac cgtcccacag
     3781 atgtactgcc tggcagtggt cgaggtggtc agacgtaaaa tgtttatcaa gcactacaga
     3841 gaatgggcag gtgccctcgt gaaagatggc aagcgtctgt atgaagccga gaaatctaag
     3901 cgtgagtcat tcggcaaact gtttgacaag agcttcctcg ataaccggct gttccgcgga
     3961 ctcgacagtt ggccacccag cttttgcacc cagaaaccta ggaagttcga ctgtgagctg
     4021 ccagatatct ccctcaagga cctgcagttt ctccagtcct tctgcccatc tgaggtgcag
     4081 ccctttctga gggtccctct gctctgtgac ttcgaacctc tgcaccagca tgtgctggcc
     4141 ctccacaacc tcgtcaaggc tgcacagagt ctggatgaga tgagccagac aatcaccgac
     4201 ctgctcagcg aacagaaggc ttctgtgtca cagacatccc cacagtctgc tagctccccc
     4261 cgtatggaga gcaccgcagg catcacaacc actacatccc cacgtacacc tccaccactc
     4321 accgtgcagg atcccctgtg ccctgctgtc tgtcctctgg aggaactctc tccagacagt
     4381 attgatgcac acactttcga ctttgagaca atccctcatc caaacatcga acagaccatt
     4441 caccaggtgt ccctggacct cgattctctg gccgagagcc ccgaatccga ttttatgtcc
     4501 gctgtgaatg agttcgtcat tgaggaaaac ctgtcttcac ccaatcctat ctcagaccca
     4561 cagagtcccg agatgatggt ggaatccctg tacagtagcg tcatcaacgc cattgattct
     4621 cggcgcatgc aggacacaaa tgtgtgcggc aaggaggact ttggagatca tacctctctg
     4681 aacgtccagc tcgaacgctg tagggtggtc gctcaggatt ctcatttctc aatccagact
     4741 attaaggagg acctgtgcca cttccgcaca tttgtgcaga aggaacagtg cgacttcagt
     4801 aacagcctga aatgtacagc cgtggagatc aggaacatca tcgaaaaggt caagtgttct
     4861 ctggagatca ccctcaaaga aaagcaccag aaagagctgc tctcactgaa gaacgaatac
     4921 gagggaaaac tggacggtct cattaaggaa accgaggaaa acgagaataa aatcaagaaa
     4981 ctgaagggag agctggtgtg cctcgaggaa gtcctgcaga acaaggataa tgaattcgcc
     5041 ctggtgaaac atgaaaagga ggctgtcatc tgcctgcaga atgagaaaga ccagaagctg
     5101 ctcgaaatgg agaacattat gcactcacag aattgtgaga tcaaagaact gaagcagagt
     5161 agagagatcg tgctggaaga tctcaagaaa ctgcatgtcg agaatgacga aaagctgcag
     5221 ctgctccgtg ccgaactcca gagcctggag cagtcccacc tcaaggaact ggaggatact
     5281 ctgcaggtgc gccatatcca ggaattcgag aaggtgatga cagaccacag agtcagcctc
     5341 gaggaactga agaaagagaa ccagcagatc attaatcaga tccaggagtc ccacgctgaa
     5401 atcattcagg aaaaagagaa gcagctgcag gagctgaaac tcaaggtgtc tgacctcagc
     5461 gatacccgct gcaaactgga agtcgagctg gccctcaagg aagctgagac tgacgagatc
     5521 aaaattctgc tcgaggaatc tcgggcccag cagaaagaaa ccctgaagtc actgctcgaa
     5581 caggagaccg aaaatctccg tactgagatc agcaaactga accagaagat tcaggataac
     5641 aatgagaatt accaagtggg actcgctgaa ctgagaactc tcatgacaat cgagaaggac
     5701 cagtgtatct ctgaactgat ttcacgtcat gaggaagagt caaacattct gaaagcagag
     5761 ctcaataaag tgactagtct gcacaaccag gccttcgaga tcgagaagaa cctgaaggag
     5821 cagatcattg aactgcagtc caagctcgat tccgaactgt ctgccctcga gaggcagaaa
     5881 gacgaaaaga tcacacagca ggaagagaag tatgaggcta tcattcagaa cctcgaaaaa
     5941 gataggcaga agctggtgtc ctctcaggag caggacagag aacagctgat tcagaaactc
     6001 aattgcgaga aggacgaagc aatccagaca gccctcaaag agttcaagct ggagagagaa
     6061 gtggtcgaga aagaactgct cgagaaagtg aagcacctgg aaaaccagat cgctaagtca
     6121 cccgcaattg atagtacccg tggtgactca agtagcctgg tggccgagct ccaggaaaag
     6181 ctgcaggaag agaaggctaa gtttctggag cagctcgaag agcaggaaaa aagaaagaac
     6241 gaagagatgc agaatgtgcg tacaagcctg atcgccgagc agcagaccaa cttcaatacc
     6301 gtcctgactc gtgagaaaat gcggaaggaa aacatcatta atgacctcag cgataaactg
     6361 aagtccacca tgcagcagca ggagcgcgac aaggatctca tcgagtccct gtctgaagac
     6421 agagctcgtc tgctcgaaga gaagaaaaag ctggaagagg aagtcagtaa gctgaggtcc
     6481 tcttcattcg tgccaagccc ctacgtcgct actgcacccg agctgtatgg agcctgcgct
     6541 cctgagctgc ctggtgaatc agaccggagt gccgtggaga ctgctgatga aggccgcgtc
     6601 gactctgcca tggagacaag catgatgtcc gtgcaggaaa acattcatat gctgtcagag
     6661 gaaaagcaga gaatcatgct gctcgagcgt accctgcagc tcaaagagga agagaacaag
     6721 cgcctcaatc agaggctgat gtcacagagt atgagtagcg tgtcctctcg ccactccgag
     6781 aagatcgcca ttagggattt ccaagtggga gacctggtcc tcatcattct ggacgaacgc
     6841 catgataact acgtgctgtt tactgtctct cctacactgt atttcctcca cagcgagtcc
     6901 ctgcccgctc tggacctcaa gcctggtgaa ggcgcatctg gcgcctcaag gagaccctgg
     6961 gtgctgggaa aagtcatgga gaaggaatac tgccaggcta aaaaggcaca gaaccgtttt
     7021 aaggtgcctc tgggcaccaa attctatcgg gtgaaggccg tctcctggaa taaaaaggtc
     7081 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
     7141 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     7201 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     7261 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     7321 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     7381 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     7441 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     7501 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     7561 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     7621 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     7681 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     7741 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     7801 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaggga
     7861 tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg
     7921 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7981 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8041 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8101 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8161 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8221 ttttggaggc ctaggctttt gcaaaaagct tgccagctgg ggcgccctct ggtaaggttg
     8281 ggaagccctg cagatctgat cccggggggc aatgagatat gaaaaagcct gaactcaccg
     8341 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     8401 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     8461 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     8521 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaatttagc gagagcctga
     8581 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     8641 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     8701 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     8761 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     8821 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     8881 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     8941 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     9001 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     9061 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     9121 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     9181 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     9241 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     9301 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga tgccgaccgg
     9361 gatctatcga tgcggcgggt gtggtggtta cgcgtcgaca atcaacctct ggattacaaa
     9421 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     9481 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     9541 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     9601 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     9661 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     9721 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9781 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     9841 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9901 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9961 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
    10021 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    10081 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
    10141 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10201 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10261 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    10321 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
    10381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
    10501 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10561 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10621 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
    10681 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12841 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    12901 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    12961 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    13021 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    13081 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    13141 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    13201 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    13261 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    13321 g
//
