LOCUS       sequence-417213-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human NPHP1 (Nephrocystin 1) gene is inserted into 
            pPB-PGK-destination (Plasmid #60436)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  TITLE     PiggyBac transposon vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPB-CAG-NPHP1
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1673
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1597..1619
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1681..1700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3815..3831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3839..3855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3863..3869,3870..3887,3888..3893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3908..3929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3989..4006)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3995..4219
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4296..4329
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   4500..4562
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(4586..4604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4625..4641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4649..4665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4673..4679,4680..4697,4698..4703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4718..4739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4856..4873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5027..5615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5107..5126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5786..6577,6578..6646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6409..6428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6647..6751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6777..7232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6914..6935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7126..7145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7359..7381
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7373..7390
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7374..7390
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7397..7416
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7397..7415
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7434..7468
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(7950..8975)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7950..8975)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8994..9493)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9042..9061)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9435..9458
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    9516..9549
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggsggggsga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1261 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcctc gacggtatcg
     1741 ataagcatgc tggcgagacg acagcgagat cctctccagg ccctgcggcg ccgcaatcag
     1801 gagctgaagc aacaggttga tagtttgctt tctgagagcc aactgaaaga agctctagaa
     1861 cccaataaaa gacaacatat ttatcaaaga tgtatccagt taaagcaggc aatagatgaa
     1921 aataaaaatg ctcttcaaaa attaagcaaa gctgatgaat ctgcacctgt tgcaaactat
     1981 aatcagagaa aagaagagga gcatactctt ttggacaagc ttacccaaca actgcagggc
     2041 cttgctgtga caataagcag agaaaatata actgaagttg gggcacctac tgaagaagag
     2101 gaagaaagtg aaagtgaaga tagtgaagac agtggtgggg aggaagaaga tgcagaggag
     2161 gaagaggaag agaaagagga aaatgaatct cacaaatggt caaccggtga agaatacatc
     2221 gctgttggag attttactgc tcagcaagtt ggagatctta catttaagaa aggggaaatt
     2281 ctccttgtaa ttgaaaaaaa acctgatggt tggtggatag ctaaggatgc caaaggaaat
     2341 gaaggtcttg ttcccagaac ctacctagag ccttatagtg aagaagaaga aggccaagag
     2401 tcaagtgaag agggcagtga agaagatgta gaggcggtgg atgaaacagc agatggagca
     2461 gaagttaagc aaagaactga tccccactgg agtgctgttc agaaagcgat ttcagagcag
     2521 ataaacactg ttgatgtgtt aactacgatg ggagctattc ctgcagggtt caggccttcc
     2581 acgctctcac agcttctgga ggaagggaat caatttcgag caaattactt cttacaacca
     2641 gagctcatgc cttcacaact ggccttcaga gatctgatgt gggatgctac agaaggcact
     2701 attaggtcga gaccaagtcg tatttcattg attctgacat tatggagctg taaaatgatt
     2761 cctcttccag gaatgagcat acaggttctc agcagacatg tacgcctctg tctatttgat
     2821 ggtaataagg ttctgagcaa cattcataca gtcagagcca catggcaacc taaaaagccc
     2881 aaaacatgga ccttttctcc ccaggttact cgcatcttac catgtttgct tgatggtgat
     2941 tgctttatca ggtctaattc tgcatctcca gatcttggaa tattatttga acttggaatt
     3001 tcttatattc gcaattcaac tggtgaaaga ggagagttaa gctgtggctg ggtgtttctt
     3061 aaactttttg atgccagtgg agttcctatt ccagcaaaaa cttatgagct tttcttgaat
     3121 ggtggtactc cttatgaaaa aggtattgaa gtggaccctt caatatccag aagagcacac
     3181 ggcagtgttt tctaccagat tatgacaatg agaaggcagc ctcaacttct agtgaaactg
     3241 agatccttga acagaagatc aagaaatgta ctaagtctac tgccagaaac attaattgga
     3301 aatatgtgtt ctattcactt gttgatattt tatcgacaaa ttcttggaga tgtgctcctg
     3361 aaagacagga tgagcttgca aagtactgat ttaattagcc atcccatgct ggccaccttc
     3421 cccatgctct tggagcagcc tgatgtgatg gatgctctca ggagttcgtg ggctggaaaa
     3481 gaaagcacat taaaaagatc agagaagaga gacaaagagt tcctgaagtc cacgtttctc
     3541 ctggtttacc atgactgcgt gctcccactt ctccactcca cacgcctacc cccattcagg
     3601 tgggcagaag aagagactga gactgcacgg tggaaagtta tcactgactt ccttaagcaa
     3661 aaccaagaaa accagggcgc cctccaagct ctgctgtcac cagacggagt tcatgaacct
     3721 tttgaccttt cagagcagac ctatgacttc ttgggtgaaa tgagaaagaa tgcagtgtga
     3781 ttgtacaaag tggtgatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3961 cgggaaacct gtcgtgccag cggatcagcc tcgactgtgc cttctagttg ccagccatct
     4021 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4081 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4141 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4201 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagatccact
     4261 agttctagcc tcgaggctag agcggccgcg tcgagataac ttcgtataat gtatgctata
     4321 cgaagttatg ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     4381 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     4441 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
     4501 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
     4561 ggttaaagag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     4621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     6781 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6841 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6901 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6961 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7021 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7081 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7141 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7201 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     7261 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7321 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7381 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacct taaccctaga
     7441 aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat
     7501 agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag
     7561 gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa
     7621 aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga taattatatt
     7681 gttatttcat gttctactta cgtgataact tattatatat atattttctt gttatagata
     7741 tccaactatg aaacattatc ataaagcaat agtaaagagc cttcagtaaa gagcaggcat
     7801 ttatctaatc ccaccccacc cccacccccg tagctccaat ccttccattc aaaatgtagg
     7861 tactctgttc tcacccttct taacaaagta tgacaggaaa aacttccatg tggacatctt
     7921 tattgtttaa tagatcatca atttctcgac tattcctttg ccctcggacg agtgctgggg
     7981 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     8041 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     8101 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     8161 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
     8221 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     8281 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     8341 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     8401 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     8461 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     8521 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     8581 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
     8641 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     8701 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
     8761 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     8821 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     8881 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     8941 tcgacagacg tcgcggtgag ttcaggcttt ttcatggtgg cggctggatc ggtcgaaagg
     9001 cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat
     9061 gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg
     9121 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat
     9181 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg
     9241 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg
     9301 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac
     9361 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg
     9421 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc
     9481 gcctccccta cccggtagaa ttcatcgatg atatcataac ttcgtataat gtatgctata
     9541 cgaagttatg cggccctcga ggtc
//
