LOCUS       sequence-417217-       11093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Penzes P, Johnson RC, Alam MR, Kambampati V, Mains RE, Eipper BA
  TITLE     An isoform of kalirin, a brain-specific GDP/GTP exchange factor, is 
            enriched in the postsynaptic density fraction.
  JOURNAL   J Biol Chem. 2000 Mar 3. 275(9):6395-403.
  PUBMED    10692441
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 3. 275(9):6395-403."
COMMENT     Sequence Label: pEAK10-His-Myc-Kal7
FEATURES             Location/Qualifiers
     source          1..11093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          897..1100
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     1057..1077
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1117..1126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1129..1146
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1153..1182
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6464..6598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6501..6520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6555..6574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(6603..7202)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6686..6706)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7183..7202
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     rep_origin      9220..9355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9850..9869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(10038..10106,10107..10898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDHLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10256..10275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(10896..11031)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga cgatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgcatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      661 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      721 ccgtgtatcg ccccgccctg ggcggcaagg ctgggagctc aaaatggagg acgcggcgct
      781 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
      841 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
      901 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
      961 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1021 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1081 gtttttttct tccatttcag gtgtcgtgaa aagcttgcca ccatggccca tcaccatcac
     1141 catcacaaca tggagcaaaa gctcatttct gaggaagatc tcaatggtgg tggtggtggg
     1201 tcgacaggat cttttcggaa tgatggtttg aaagcttctg atgtccttcc tatcctaaag
     1261 gagaaagtgg cctttgtgtc tgggggtcgt gataagcgag gtggacccat cctgaccttc
     1321 cctgcccgaa gcaatcatga cagaataaga caggaagacc tacggaaact cgtgacgtat
     1381 ttggccagcg tgccaagcga ggacgtttgc aaacgtggct tcaccgtcat catcgacatg
     1441 cggggctcca agtgggacct catcaagccc ctcctcaaga cgctgcagga agcctttccc
     1501 gccgagatcc atgtggccct catcatcaag cctgacaact tctggcagaa gcagaagacc
     1561 aactttggca gctccaaatt catctttgag acgagcatgg tgtctgtgga gggcctcacg
     1621 aagctggtgg acccctccca gctgacagaa gagtttgacg gttccctgga ttacaaccac
     1681 gaggagtgga ttgagttgcg cctctccctg gaggagttct tcaacagtgc ggtccacctg
     1741 ctctcacgcc tcgaggacct gcaggagatg ctggcccgga aggagttccc tgtggatgtg
     1801 gagggctctc ggaggcttat cgacgagcac acacaactta agaaaaaggt gctgaaggcc
     1861 cccgtggaag agctggaccg ggaggggcag cggctactgc agtgtatccg ctgcagtgat
     1921 ggcttctcag ggcgcaactg catcccagga agtgccgact tccagagcct tgtacccaag
     1981 atcaccagcc tcctggacaa gctgcattcc acccgacaac acctgcacca gatgtggcac
     2041 gtccgaaagc tcaagctgga tcagtgcttc cagctgcggc tcttcgagca ggatgccgag
     2101 aagatgttcg actggataag ccacaacaag gagttatttc ttcagagcca cacagagatc
     2161 ggagtcagct accagcatgc actagacctc cagacacagc acaatcactt tgccatgaac
     2221 tccatgaatg cctatgtcaa catcaaccgc atcatgtctg tcgcatcccg cctctcagag
     2281 gctggccatt atgcctccca gcaaatcaag cagatctcca cgcagctaga ccaggagtgg
     2341 aagagctttg ctgctgccct ggatgaacgc agtacaatcc tggccatgtc tgctgtgttc
     2401 caccagaagg cagagcagtt cctgtcagga gtggatgcct ggtgtaagat gtgcagtgaa
     2461 ggcggccttc cttctgagat gcaggacctg gagctggcaa tccaccatca ccagtccttg
     2521 tatgagcagg tgacccaggc ctacacagag gtcagccagg atgggaaagc actcctggat
     2581 gtcctgcagc gtcccctgag ccctgggaac tccgagtccc tcacagccac agccaactac
     2641 tccaaggcgg tacaccaggt gctggatgtg gtacatgagg tgctgcacca ccagcggcgg
     2701 ctcgagagca tctggcagca ccgtaaagtg cggcttcacc agcggctgca gctgtgcgtc
     2761 ttccagcagg atgtgcagca ggtgctggac tggattgaaa accacggtga ggcctttctc
     2821 agcaaacaca ctggggtcgg caagtccctg catcgggcac gggcattgca gaagaggcat
     2881 gatgactttg aagaggtggc tcagaataca tacaccaatg cggacaagct cctagaggca
     2941 gcagagcagc tggctcagac tggggaatgt gacccggaag agatctacaa ggctgcccga
     3001 cacctggagg tgcgcatcca agacttcgtg cgcagagtgg agcagcggaa gcttcttctg
     3061 gacatgtccg tctctttcca cacgcacacc aaagagctgt ggacatggat ggaagacctt
     3121 cagaaggagg tgctggagga tgtctgtgca gactctgtgg atgcggtcca ggaactgatc
     3181 aagcagttcc agcagcagca gaccgctacc ctggacgcca ctctcaacgt catcaaggaa
     3241 ggcgaagacc ttatccagca gctcaggtca gcgcctccct ccctggggga gcccaccgag
     3301 gccagggact cagctgtatc caacaacaag acgccccaca gtagttccat cagccatatc
     3361 gagtctgtcc tccagcagct ggacgatgct caggtacaga tggaggagct gtttcacgag
     3421 cggaagatca agctggacat cttcctgcag ctgcgcatct tcgaacagta caccattgag
     3481 gtcaccgcag agctggatgc ctggaatgag gacttgctcc ggcagatgaa tgacttcaac
     3541 acagaggacc tgaccctggc agagcagcgg ctacagcgcc acacagaacg gaagcttgcc
     3601 atgaacaaca tgacattcga ggtcatccag cagggccagg acctgcacca atatatcatg
     3661 gaagtccaag cctcaggaat cgagttaatc tgtgagaagg acgttgacct ggcagctcag
     3721 gtgcaagagc tactggaatt tctccatgag aagcagcacg aactggagct caatgcggag
     3781 cagacgcaca agcggctaga gcagtgtctc cagctgcggc acctccaggc tgaggtcaaa
     3841 caggttctgg gatggatccg caatggagag tccatgctca acgccagcct ggtcaatgcc
     3901 agctctttgt ctgaggcaga gcagctacag cgggagcacg aacagttcca gctagccatc
     3961 gagaagacgc accagagcgc cctgcaggta cagcagaagg cagaggcact gctccaggcc
     4021 ggccactatg acgccgatgc tatccgggaa tgtgcggaga aggtggccct ccactggcaa
     4081 cagctcatgc taaagatgga ggaccggctg aaactcgtca atgcctctgt ggccttttac
     4141 aagacctctg agcaggtgtg cagcgttctg gagagcttag agcaggagta ccggagagac
     4201 gaagactggt gcggcgggag agacaagctg gggccggcgg cagagatgga ccacgtcatt
     4261 ccactcctca gcaagcatct ggagcagaag gaggccttcc tgaaggcctg cactctggca
     4321 cggcggaatg ccgaggtgtt cctcaagtac atccacagga acaatgtgag catgcccagt
     4381 gttgccagcc acaccagggg acccgaacag caagtaaaag ccatcttgag tgagctcttg
     4441 cagagagaga atcgcgtcct gcacttctgg accttgaaga agcggcggtt agaccagtgt
     4501 cagcaatatg tggtgtttga acgcagtgct aaacaggcac tagactggat ccaagaaaca
     4561 ggagaatatt acctctcaac ccatacctcc actggagaga ccacagagga gactcaggag
     4621 ctgctgaaag agtatggaga attcagggtg cctgccaagc aaacaaaaga gaaggtgaag
     4681 cttctgatcc agctagcaga cagctttgtg gaaaagggcc acatccatgc cacagagata
     4741 cggaaatggg tgaccacggt ggacaagcac taccgggatt tctccctgag gatggggaag
     4801 taccggtaca ccctggagaa agccctggga gtcaacacgg aggataacaa ggacctagag
     4861 ctggacatta tcccagcaag cctttcagac cgggaggtca agctccggga tgctaaccac
     4921 gaggtcaatg aagagaaacg gaagtcagct cgaaagaaag aattcattat ggctgaactg
     4981 ctccagaccg agaaggctta tgtaagagac ctacacgagt gcttggagac ctacctgtgg
     5041 gaaatgacca gtggtgtgga agagatacca cctgggatcc tcaataagga gcacatcatc
     5101 ttcggcaaca tccaggagat ctatgacttc cacaacaaca tcttcctcaa agagctggag
     5161 aagtacgagc agctgcctga ggatgtggga cactgctttg ttacctgggc agacaaattt
     5221 cagatgtatg tcacctactg taaaaacaag cctgattcca accagctgat cctggagcac
     5281 gcaggcacct tctttgatga gatccagcag cggcacgggc tggccaactc catctcttcc
     5341 tacttaatta agcctgtcca gagagttacg aagtaccagc tgctcctgaa ggaactttta
     5401 acttgctgtg aagaagggaa aggggagctc aaggatggcc tggaggtgat gctcagtgtc
     5461 cccaagaaag ccaatgatgc catgcatgtc agcatgcttg aagggttcga tgagaacttg
     5521 gatgtacagg gggagttgat tctccaggat gcctttcaag tgtgggaccc aaagtcgctg
     5581 atccggaagg ggcgggaacg gcatctgttc ctctttgaga tctccttggt gtttagcaaa
     5641 gagatcaaag actcttcagg acacacgaaa tatgtttaca agaacaagct actgacctca
     5701 gagctgggtg tgaccgagca cgtagaaggt gacccctgca aattcgcctt gtggtctggg
     5761 cgcaccccat cctcagacaa taaaacagtg ctcaaagctt ccaacatcga aaccaagcag
     5821 gaatggatca agaacattcg ggaagtgatt caggaaagga tcattcatct taaaggagct
     5881 ttaaaggagc caatccagct ccccaaaaca ccagccaaac tgaggaacaa tagtaagagg
     5941 gatggggtag aggatggtga cagccagggg gacgggagca gccagcccga taccatatcc
     6001 attgcctcca ggacctctca gaacacagtg gagagtgaca aggatggcaa ccttgttcct
     6061 cggtggcacc tgggacctgg agatcctttc tccacttacg tttagcgcgc agcctgggac
     6121 ttgtccctgc agctcaccgg gttagccgtc tagagcggcc gcaggtaagc cagcccaggc
     6181 ctcgccctcc agctcaaggc gggacaggtg ccctagagta gcctgcatcc agggacaggc
     6241 cccagccggg tgctgacacg tccacctcca tctcttcctc aggtctgccc gggtggcatc
     6301 cctgtgaccc ctccccagtg cctctcctgg tcgtggaagg tgctactcca gtgcccacca
     6361 gccttgtcct aataaaatta agttgcatca ttttgtttga ctaggtgtcc ttgtataata
     6421 ttatggggtg gaggcgggtg gtatggagca aggggcccaa gttaacttgt ttattgcagc
     6481 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6541 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     6601 cttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     6661 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     6721 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     6781 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     6841 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagcctg
     6901 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     6961 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     7021 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     7081 cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     7141 cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     7201 atggtggcct gcagagtcgc tctgtgttcg aggccacacg cgtcacctta atatgcgaag
     7261 tggacctggg accgcgccgc cccgactgca tctgcgtgtt ttcgccaatg acaagacgct
     7321 gggcggggtt tgtgtcatca tagaactaaa gacatgcaaa tatatttctt ccggggacac
     7381 cgccagcaaa cgcgagcaac gggccacggg gatgaagcag ctgcgccact ccctgaagat
     7441 ccatcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct
     7501 ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga
     7561 aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag
     7621 gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat
     7681 atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt
     7741 tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat
     7801 tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca
     7861 tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta
     7921 gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga
     7981 ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgacccccg
     8041 ctgcgcgggg gcacgtcagg ctcaccatct gggccgcctt cttggtggta ttcaaaataa
     8101 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     8161 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     8221 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     8281 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     8341 cctcctcgac cccggcctcc acctcctgct cctgcccctc ccgctcctgc tcctgctcct
     8401 gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca
     8461 ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct
     8521 cgtcctcgtc ctcttccccg tcctcgtcca tgtgccatga tggcggcctg cagctgtgtt
     8581 cgaggccgcg cgtgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg
     8641 catctgcgtg ttcgagttcg ccaatgacaa gacgctgggc ggggagatcc cccttattaa
     8701 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     8761 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     8821 gtcctaagga acagcgatct ggatagcata tgctatccta atctatatct gggtagcata
     8881 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     8941 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     9001 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     9061 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     9121 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaaggag
     9181 atctgtcgac atcgatgggc gcgggtgtac actccgccca tcccgcccct aactccgccc
     9241 agttccgccc attctccgcc tcatggctga ctaatttttt ttatttatgc agaggccgag
     9301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9361 ttttgcaaaa agctaattcg gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9481 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     9541 caagaactct gtagcaccgc ctacatacct cgctctgctg aagccagtta ccagtggctg
     9601 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagagatagt taccggataa
     9661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9961 cgcaagctag agtttaaact tgacagatga gacaataacc ctgataaatg cttcaataat
    10021 attgaaaaag gaaaagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgcag
    10141 aagatcactt gggtgcgcga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10201 ttgagagttt tcgccccgaa gaacgtttcc caatgatgag cacttttaaa gttctgctat
    10261 gtggcgcggt attatcccgt attgatgccg ggcaagagca actcggtcgc cgcatacact
    10321 attctcagaa tgacttggtt gaatactcac cagtcacaga aaagcatctt acggatggca
    10381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10441 tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgaaaacta ttaactggcg
    10621 aactacttac tctagcttcc cggcaacaac taatagactg gatggaggcg gataaagttg
    10681 caggaccact tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatcaggag
    10741 ccggtgagcg tgggtcacgc ggtatcattg cagcactggg gccggatggt aagccctccc
    10801 gtatcgtagt tatctacact acggggagtc aggcaactat ggatgaacga aatagacaga
    10861 tcgctgagat aggtgcctca ctgattaagc attggtaagg ataaatttct ggtaaggagg
    10921 acacgtatgg aagtgggcaa gttggggaag ccgtatccgt tgctgaatct ggcatatgtg
    10981 ggagtataag acgcgcagcg tcgcatcagg catttttttc tgcgccaatg caaaaaggcc
    11041 atccgtcagg atggcctttc ggcataacta gtgaggctcc ggtgcccgtc agt
//
