LOCUS       sequence-417243-        8708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for APCDD1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   You Z, Wang L, He H, Wu Z, Zhang X, Xue S, Xu P, Hong Y, Xiong M, 
            Wei W, Chen Y
  TITLE     Mapping of clonal lineages across developmental stages in human 
            neural differentiation.
  JOURNAL   Cell Stem Cell. 2023 Apr 6;30(4):473-487.e9. doi: 
            10.1016/j.stem.2023.02.007. Epub 2023 Mar 17.
  PUBMED    36933556
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.02.007"; journalName: "Cell Stem Cell"; date: 
            "2023-04-6- 6"; volume: "30"; issue: "4"; pages: "473-487.e9"
COMMENT     Sequence Label: APCDD1-P2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..8708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..444)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(126..147)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     338..357
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        471..575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(576..644,645..1436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(794..813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2096..2115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2349..2366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2483..2504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2519..2524,2525..2542,2543..2549)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2557..2573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2562..2584
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2581..2597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2581..2597
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2618..2636
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3635..3691
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3692..5122
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    5156..5189
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5211..5687
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5410..5429)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        5748..6247
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5783..5806)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6180..6199
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6287..6886
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6287..6306)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    7443..7476
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7502..7518)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7502..7518)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8514..8533)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8515..8533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8540..8557)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8540..8556)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8549..8571)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
       61 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      421 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2581 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     2641 aaaagctggt accttcttgc ttgcagccta acttatttta gatgcctgat gtatgggccc
     2701 ataggaatgt gtgagaatgc caaccagaat aggtgtttga caccaaaact tttagaaacc
     2761 cgaaaacctt ttgataaaag caaaattcca gcctttgttg aatatttttc tttcaatccc
     2821 aacgagtcag catcaccaaa taaaactcct taggagcact ccgagctgta gttttatttg
     2881 ttgcctgcag gcactcccag ctgtattctg ggctcagtgt ttctacacca gatggtctgt
     2941 gttgcgtggc cacacctgca gcatatcggt gtgactgcca cccagatcaa caggttgaac
     3001 tcttctccgc ctgacagtgc tttgatgact actgccgtca gatgggtggg tctgtaggtg
     3061 ggattgtttt tggaaaagaa agccttgtct agttagagtg tggccagtgt tttctgggtg
     3121 ggtttctgga tcccacgcct actccctgga gtcatggaac tctcctttct cctttgcagt
     3181 gaatcacatg aaggtcaccc ccatggatgc ggccacagcc tcactgctca acgtcttcaa
     3241 cgggaatgag tgcggggccg agggctcctg gcaggtgggc atccagcagg atgtgaccca
     3301 caccaatggc tgcgtggccc tgggcatcaa actacctcac acggagtacg agatcttcaa
     3361 aatggaacag gatgcccggg ggcgctatct gctgttcaac ggtcagaggc ccagcgacgg
     3421 gtccagccca gacaggccag agaagagagc cacgtcctac cagatgccct tggtccagtg
     3481 tgcctcctct tcgccgaggg cagaggacct cgcagaagac agtggaagca gcctgtatgg
     3541 ccgggcccct gggaggcaca cctggtccct gctgctggct gcacttgcct gtcttgtccc
     3601 tctgctgcat tggaacatcc gcagaggatc cggcgcaaca aacttctctc tgctgaaaca
     3661 agccggagat gtcgaagaga atcctggacc gatggtgagc aagggcgagg aggtcatcaa
     3721 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     3781 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3841 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     3901 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     3961 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     4021 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     4081 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     4141 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     4201 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     4261 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     4321 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
     4381 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
     4441 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     4501 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4561 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     4621 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     4681 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     4741 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     4801 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     4861 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     4921 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     4981 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     5041 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     5101 catggacgag ctgtacaagt aatgagaatt ccgatcatat tcaataaccc ttaatataac
     5161 ttcgtataat gtatgctata cgaagttata gcgctgctcg agagatctac gggtggcatc
     5221 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     5281 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     5341 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     5401 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     5461 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     5521 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     5581 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     5641 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtcctttag gtctgaagag
     5701 gagtttacgt ccagccacgc gtaggatctc gacctcgaaa ttctaccggg taggggaggc
     5761 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5821 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccgactcc
     5881 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5941 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6001 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6061 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6121 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6181 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6241 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
     6301 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6361 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6421 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6481 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6541 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6601 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6661 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6721 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6781 ccacaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6841 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     6901 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg
     6961 ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc
     7021 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
     7081 gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc
     7141 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
     7201 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
     7261 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
     7321 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
     7381 tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagatcc ggaaccctta
     7441 atataacttc gtataatgta tgctatacga agttattagg tccctcgaag aggttcacta
     7501 ggatccacta gttctagagc ggccgctaga agttttagaa agttctattt ttccattggt
     7561 cctaagctta ctattgacag attttcttta ccaaaagaaa agacatttat tcttttgatg
     7621 cacttgaatg ccagagaact gtccttcttt ttctcctctc cctccctccc agcccctgag
     7681 tcatgaacag caaggagtgt ttgaagtttc tgctttgaac tccgtccagc ctgatccctg
     7741 gcctgagcaa cttcacaaca gtaattgcac tttaagacag cctagagttc tggacgagcg
     7801 tgtttggtag cagggatgaa agctagggcc tcttattttt ttctcttaat tattattata
     7861 tttctgagtt aaacttagaa gaaacaacta tcaagctaca acttttcctg ccattttcct
     7921 gtggttgcag cctgtcttcc tttgaaattg ttttactctc tgagttttat atgctggaat
     7981 ccaatgcaga gttggtttgg gactgtgatc aagacacctt ttattaataa agaagagaca
     8041 caggtgtaga tatgtatata caaaaagatg tacggtctgg ccaaaccacc ttcccagcct
     8101 ttatgcaaaa aaaggggaga atcaaagctt tcatttcaga aatgttgcgt ggaaaagtat
     8161 ctgtaattaa agtttcgaag taatttaacc tatttttaca tcatttgtct taccttgttg
     8221 agagaggaga ctcgcttgtt ggcttctggt tgttgcagga ggcatgtggc ttcctagcct
     8281 tctcagtaga caggatcatt aggaaaatag caacatcaat cctgttacac aggagcagtg
     8341 acacgggtgg ctgcagtgtg gtacatgcca cacaaatgat agagaaagtg cccgttcatt
     8401 gcagtttcct cttcctgggg gacctgagcc ctgactcact gtcttattag gggcaggcgg
     8461 aagaagctgc actctggcat ccatgccacc ttctaagatg gagctccaat tcgccctata
     8521 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8581 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8641 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat
     8701 tgtaagcg
//
