LOCUS       sequence-417246-        8083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal Fluorescent Surrogate Reporter for adenine and cytosine 
            base editors. Key Construct: CMV-mCherry-CMV-eGFP* 
            (ATAACG)-hU6-gRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma L, Xing J, Li Q, Zhang Z, Xu K
  TITLE     Development of a universal antibiotic resistance screening reporter 
            for improving efficiency of cytosine and adenine base editing.
  JOURNAL   J Biol Chem. 2022 Jul;298(7):102103. doi: 10.1016/j.jbc.2022.102103.
            Epub 2022 Jun 6.
  PUBMED    35671823
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-07"; volume: "298(7):102103. doi"; pages: " 
            10.1016/j.jbc.2022.102103. Epub 2022 Jun 6"
COMMENT     Sequence Label: pACBE-FSR (pXK-1648)
FEATURES             Location/Qualifiers
     source          1..8083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(154..222,223..1014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(372..391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1185..1773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1674..1693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1927..1944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2061..2082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2097..2102,2103..2120,2121..2127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2135..2151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2140..2162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2159..2175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2159..2175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2196..2214
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2363..2397
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2810..3113
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3114..3317
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3345..3354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3351..4061
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3496..3514)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3764..3783
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4062..4650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4115..4135)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4533..4544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4686..4703)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4692..4916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5028..5331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5332..5535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(5583..5585,5586..6296)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5583..5585,5586..6296)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5625..5646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5886..5905)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6233..6254
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6435..6556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6472..6491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6526..6545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6569..6817
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6569..6589
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6740..6759
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6838..6913
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     repeat_region   7339..7401
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7467..7486)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7468..7486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7493..7510)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7493..7509)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7502..7524)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7651..8079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7738..7757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7948..7969
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
       61 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      301 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      361 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      481 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      541 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      601 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      661 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      721 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
      781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      841 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
      901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
      961 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1021 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1081 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1321 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1801 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1861 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1921 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     1981 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2041 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2101 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2161 ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa
     2221 aagctggtac ctcgcgcgac ttggtttgcc attctttagc gcgcgtcgcg tcacacagct
     2281 tggccacaat gtggtttttg tcaaacgaag attctatgac gtgtttaaag tttaggtcga
     2341 gtaaagcgca aatctttttt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
     2401 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
     2461 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
     2521 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
     2581 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
     2641 attatatata tattttcttg ttatagatat cccggaattg catgaagaat ctgcttaggg
     2701 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata taggtaccca ttaattaaat
     2761 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     2821 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2881 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2941 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
     3001 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
     3061 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     3121 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     3181 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     3241 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
     3301 tctatataag cagaggtcgt ttagtgaacc gtcagatcac gcgtgccacc atggtgagca
     3361 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     3421 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     3481 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     3541 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     3601 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     3661 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     3721 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3781 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     3841 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     3901 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     3961 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     4021 gccgccactc caccggcggc atggacgagc tgtacaagta aaatcaacct ctggattaca
     4081 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4141 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4201 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4261 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4321 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4381 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4441 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4501 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4561 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     4621 gtcggatctc cctttgggcc gcctccccgc caccggttag gggcccgttt aaacccgctg
     4681 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4741 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4801 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4861 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcggc
     4921 gcgcctaaaa gttttgttac tgacattgat tattgactag taagctttag ttattaatag
     4981 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     5041 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     5101 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     5161 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     5221 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     5281 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     5341 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     5401 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     5461 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     5521 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgggataa
     5581 cggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5641 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     5701 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     5761 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     5821 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     5881 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     5941 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6001 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     6061 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     6121 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6181 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     6241 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
     6301 gactcagatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     6361 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6421 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6481 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6541 tcatcaatgt atcttactcg aggaattcga gggcctattt cccatgattc cttcatattt
     6601 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     6661 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     6721 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     6781 cttggcttta tatatcttgt ggaaaggacg aaacaccgat aacggtgagc aagggcggtt
     6841 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     6901 accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc
     6961 gtttttagcg cgtgcgccaa ttctgcagac aaatggcgga tcccgagagc ataatattga
     7021 tatgtgccaa agttgtttct gactgactaa taagtataat ttgtttctat tatgtatagg
     7081 ttaagctaat tacttatttt ataatacaac atgactgttt ttaaagtaca aaataagttt
     7141 atttttgtaa aagagagaat gtttaaaagt tttgttactt tatagaagaa attttgagtt
     7201 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     7261 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     7321 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
     7381 aatatgatta tctttctagg gttaaataat agtttctaat ttttttatta ttcagcctgc
     7441 tgtcgtgaat accgagctcc aattcgccct atagtgagtc gtattacaat tcactggccg
     7501 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7561 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7621 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     7681 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7741 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7801 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7861 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7921 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7981 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8041 taaaaaatga gctgatttaa caaaaattta acgcgaattt taa
//
