LOCUS       sequence-417250-       11337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring
            the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA.
            Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Xu K, Ren C, Liu Z, Zhang T, Zhang T, Li D, Wang L, Yan Q, Guo L, 
            Shen J, Zhang Z
  TITLE     Efficient genome engineering in eukaryotes using Cas9 from 
            Streptococcus thermophilus.
  JOURNAL   Cell Mol Life Sci. 2015 Jan;72(2):383-99. doi: 
            10.1007/s00018-014-1679-z. Epub 2014 Jul 20.
  PUBMED    25038777
REFERENCE   2  (bases 1 to 11337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-014-1679-z"; journalName: "Cell Mol Life Sci"; date:
            "2015-01"; volume: "72"; issue: "2"; pages: "383-99"
COMMENT     Sequence Label: pLL3.7-hSt3Cas9 (pXK-1721)
FEATURES             Location/Qualifiers
     source          1..11337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        106..409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    525..558
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(600..616)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(736..1048)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(797..817)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    1107..1224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1522..1566)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1751..1984
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2481..2606
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2653..2833
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2851..3049)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3051..3430
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3602..3621
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3696..3800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3801..3869,3870..4661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4019..4038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4832..5420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5321..5340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             5482..5662
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     6172..6188
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6191..6779
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6297..6308
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6706..6726
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6835..6868
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6891..6938)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(11109..11129)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11136..11201)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(join(11239..11337,1..105))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
       61 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
      121 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
      181 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
      241 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
      301 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
      361 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
      421 atatgggcta tgaactaatg accccgtaat tgattactat taataactaa tgcatggcgg
      481 taatacggtt atccacgcgg ccgcttaagc ttggaaccct taatataact tcgtataatg
      541 tatgctatac gaagttatta ggtccctcga cctgctggaa tctcgtgaag cgagcttatc
      601 gataccgtcg acctcgagaa aaaaaacacc gaatcggtgc caccttttca agttgagtac
      661 ggactaagcc ttattttaac tcgctgttgg atcctacagc tctaaaacgc gctgcgagcc
      721 taggcgaagg atccaaacaa ggcttttctc caagggatat ttatagtctc aaaacacaca
      781 attactttac agttagggtg agtttccttt tgtgctgttt tttaaaataa taatttagta
      841 tttgtatctc ttatagaaat ccaagcctat catgtaaaat gtagctagta ttaaaaagaa
      901 cagattatct gtcttttatc gcacattaag cctctatagt tactaggaaa tattatatgc
      961 aaattaaccg gggcagggga gtagccgagc ttctcccaca agtctgtgcg agggggccgg
     1021 cgcgggccta gagatggcgc gtcggatctc tagagcgggc ccggtactaa ccaaactgga
     1081 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
     1141 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
     1201 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct gcagaattgg
     1261 cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     1321 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     1381 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     1441 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     1501 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     1561 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     1621 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     1681 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     1741 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     1801 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     1861 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     1921 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     1981 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     2041 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     2101 cctcatatct cctcctccag gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc
     2161 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     2221 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     2281 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     2341 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     2401 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     2461 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     2521 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     2581 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     2641 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     2701 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     2761 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     2821 accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca ccgtacacgc
     2881 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
     2941 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
     3001 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
     3061 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     3121 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     3181 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     3241 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     3301 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     3361 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     3421 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
     3481 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
     3541 ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat
     3601 gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga tctcccgatc
     3661 cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5461 gctggccttt tgctcacatg ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     5521 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     5581 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     5641 tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc atgtgatgaa
     5701 atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca tccatcccat
     5761 gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct tctaccttct
     5821 cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgatccctg
     5881 gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag atccacagat
     5941 caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
     6001 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctgaggtg
     6061 tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa tcagcattgg
     6121 tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga tcgaggtcga
     6181 cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     6241 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     6301 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     6361 agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac
     6421 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     6481 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     6541 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     6601 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     6661 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     6721 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta
     6781 tcgataagct tgatatcgaa ttgtacctag tggaaccgga acccttaaac atgtataact
     6841 tcgtataatg tatgctatac gaagttatta ggtccctcga cgaattctta tttctttttc
     6901 ttagcttgac cagctttctt agtagcagca ggacgcttgc cttctcccag tttagccagg
     6961 tcaatccgag tctcatacag cccagtcacg ctctgatgaa tcagagtggc atccttcagc
     7021 aggcttgatg gtgtgtagtc tcgatagcga gggattttca cgcccagaaa ctcgaaatca
     7081 gcggcggagc cgcggctggt cagttcaaac agtcccttcc tctcactgcc agtaggtccg
     7141 atgaagctgc tgcacagctc gtcgatagag tgattctgcc aggactggaa ggcgctgttc
     7201 agcagctttc catttttctt tgcccccacg tagttttcat taaactccag gatgtagtaa
     7261 aacagttcct cgaactcttt cttatgattc tccacatact tgcggtggtt ctcattaatt
     7321 gtgttggaga tccttttagc atggtacagc agcttcacaa acttctggct caggaaaatc
     7381 tggttgcctt tgtggatctc tccccgctta ttgttggttg acaggatact ggccagcatg
     7441 cgcctagacc catcggacag ctcgaacagg gagtacttag gcagttcaat gatcagctca
     7501 atgtccttat agcctttctc cagcaggaag ttcagcttat cttttcggta attgatgcgg
     7561 tccagaatgc tgattccctg aaattccagc acatttgtaa ttttcttttt ggcccctttc
     7621 tcgatggtgc ccttgaccag cacagcgaag gagttgctga tgccggcgta tcccccatac
     7681 ttcttggggt ccaggtattc cttagctccc accaggttct cattagagtt aggcttgggt
     7741 ttggagctca gattggcgtt aaacaggccc tttggttttc cgcggtccag cccgtgattc
     7801 tgttcctcga cctttttgac cacgttcacc tgagggtagg acaggactct ccgcactgtg
     7861 gccagatctg attccttatt ccacacgctc tccccggttt cctcgttgac ttcaatcagg
     7921 ggcctctcga tcactctgcc gtcggccagt gagatgctct tcttgaagat gttcatgatg
     7981 ttgctataga agtacacctt ttctgttgca gacttcctct ctctaaagga gttgtacttt
     8041 ggatagtcgc cgtacacgaa ctctggctcc agtttagggt actttttcag cagagcagag
     8101 gcgaccacgg cattcagata ggcatcatgt gcatggtgga agtcgttgat ttctctcact
     8161 ttgtacagct caaagtcctt ccggaactgt gagaccaggg tacttttcag agtaatgatc
     8221 ttgacggtcc gcactgctcg attgttttca tccttcttgt tgttgaactt ctcgtccagc
     8281 agtctagcca cgtgttttgt aatctggcgg gtctcgacca gctgcctctg gataaagcct
     8341 gccttatctt cggggctcag gcctcctctc tcagctttag tcaggttgtc aaactttcgc
     8401 tgagagatca gcttggattt cagcagctga taccagaatg tcttcctctt tttgaccacc
     8461 tccagggaag gcacatcgtc gctttttcct ctatttgagg ctgaactgac cagcaccttg
     8521 ttatcgatgg aattgtcttt cagaaatgcc tggggaatga tgtgatcaat gtcgtagtta
     8581 ctcagccgat cgatgtccag atcgtcgcct gtgtacatgt cctttccatt ctggaggtag
     8641 tacagataca ggcgatcgtt ctggagtgca ttgttgtcga tcttggacag tttagctgga
     8701 atattctcct tcaggatttt gcttcccagt tccttcagtg atttctccag gcgcttcagc
     8761 ctctgctgag agttggattt cccctggttg gtgtactgat tttctctagc catctcgacc
     8821 acgatgcttt caggttttct gccgcccatg accttcacca gctcgtccac aatcttgata
     8881 gactggagga tgcccttttt gatggcgggg ctgcctggca gtgatttgac cacttccttg
     8941 atgtttcctt tatcctcgtc cccaatgatc tgagccttct ggatcttctt cttgaagctc
     9001 agggcatcgt cgtggatcag ctgcatgaaa ttcctgttac taatgccatc gtcgatcaga
     9061 taatccagga ttgtatttcc agacttctcg tcccggatgc cgttgatcag cttggcggac
     9121 agtttccccc agccggtgta atgtcggcga ctcagtttct tcagcacaga cttatcaaag
     9181 atgttctcga atttgctcag tctctgctta atcatttccc ggtcctcgaa gatggtcaga
     9241 gtgtgaatga tttcctcaat gatggcttca ttagaggaat cgtccaggaa ctccttatcg
     9301 ttaatgatat tcagcaggtc atggtaagtg gacaggcttg agttaaactg cttttcaatg
     9361 cctttcagct cgatcccgtc atagccgtag atagcgtgca gatactcaat gatatccttg
     9421 tctgtcacct tccttttatc cttgaagtac agtctgacaa tgtctttctt ctgcttggaa
     9481 tccagaaact gatagtcccg catgctctcg gcgatgaatc ggacctttgt cagttcgttg
     9541 tacacgttga aggtctcgta cagcaggcta tgcttgggca gcactttttc ctctggcagg
     9601 tacagatcaa atgaggtcat cctgttaatg aatgcttcag cactagactc cttatcgatc
     9661 acgtcttcga agttccatgg agtaatcttc tcattccgtt ttcggatact ccatgcaaag
     9721 tcagaattgc ctctagccag aggtcccaca tagtagggga ttctgaaggt caggatcttt
     9781 tcgatgcgct ctttgttctt ggccagaaag ggatagaact tggcctgttt atccaggatt
     9841 gccctcatct cctggaggtg aatctggtat gggatgctgc cgttgtcaaa agttctctgc
     9901 ttccgcagga aatcttctct gtcaattttc tccagaaaat agtcggcccc ttcgaacttt
     9961 gccagcagtt tcttcagata cacgtagaaa tcctcctgat ttgttttgcc gtcgatatat
    10021 cctgcgtacc cgttcttggt atcgtcttta aacacttcgt tgtaagtctt cagggaaata
    10081 ttccggatat actctttcag cagagccaga tcttctttat gctcattgta ccgcttgatc
    10141 atagcggagc tcaggggggc ttcggtctcg ttgtctgtca cggtcagaaa gcctgacagc
    10201 agaatagcat catacagttt cttggccttc aggaacacgt cactgtaatc gtcgccgata
    10261 tatcccagca gagtttccag atcctcgtcg tagctttcct ttgagaagtg caggctggct
    10321 ttctcatcca gattaaagca cttccggaag tcggcctggt tccccacaat cagcttcaga
    10381 aattcactga agatcccaga atttttctcg cctgggaaca gcttcaggat gcgatctttc
    10441 ttctccagct tgctaatttt gtccttcacg atttcctcca gctgttttga gttttccaga
    10501 ctcagatcag actcaaaaat ggcattgtag gtatccagaa agtcctggaa gtttttctgg
    10561 atgtcattgt tcttagagtt gaactccccc tcaatcagga aatggccccg gtacttgatc
    10621 atgtgggcca gtgccagata caccaggcgc agatctgctt tcttagtgga gtcagccagg
    10681 tacttcctca gatggtagat tgtaggaaac tcatcgtggt aggctttttc ctccaccaga
    10741 ttgccgaaga taggatactt actgtctctt ttatcgtcgg ggaccaggaa agaatcgtcc
    10801 agccgctgaa agaatgcatc gtccagagta gccatttctg tgctgaagat ctcctggagg
    10861 tacaggatgc ggttccttct ccgagtatat cggcgcctgg ctgtcctctt cagtctccgt
    10921 ccctctgcgg taatcccaga gtcgaacagc agcactccca gcaggttttt cttgatgtat
    10981 ttcttggaag tgttccccag gaccttcatt ttctttgatg gcactttgta gttgtcagta
    11041 gtcacggccc accccacgct gtttgttcca atgtccagtc caatgctgta tggtttagtg
    11101 accggtagga ccttccgctt cttctttggc ccgggcttat cgtcatcgtc tttgtaatca
    11161 atatcatgat ccttgtagtc tccgtcgtgg tccttatagt ccgccatggt gctagcggat
    11221 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
    11281 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttg
//
