LOCUS       sequence-417286-        4061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector for inserting MPRA libraries into AAV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   McAfee JC, Lee S, Lee J, Bell JL, Krupa O, Davis J, Insigne K, Bond 
            ML, Zhao N, Boyle AP, Phanstiel DH, Love MI, Stein JL, Ruzicka WB, 
            Davila-Velderrain J, Kosuri S, Won H
  TITLE     Systematic investigation of allelic regulatory activity of 
            schizophrenia-associated common variants.
  JOURNAL   Cell Genom. 2023 Sep 15;3(10):100404. doi: 
            10.1016/j.xgen.2023.100404. eCollection 2023 Oct 11.
  PUBMED    37868037
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2023-09-15"; volume: "3(10):100404. doi"; pages: " 
            10.1016/j.xgen.2023.100404. eCollection 2023 Oct 11"
COMMENT     Sequence Label: pAAV-MPRA-MluI-SpeI-EcoRI
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      765..1353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1254..1273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1415..1544
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    1602..2190
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1655..1675)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2073..2084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2222..2698
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2421..2440)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2738..2878
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2738..2867
                     /label=AAV2 ITR
     rep_origin      2953..3408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3040..3059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3250..3271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3425..3444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3544..3566
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3604..3622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3690..3794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3795..3863,3864..4061,1..594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4013..4032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
       61 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      121 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      181 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      241 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      301 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
      361 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      421 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
      481 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
      541 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
      601 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      661 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      721 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      781 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      841 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      901 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      961 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1021 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1081 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1141 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1201 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1261 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1321 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1381 ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg
     1441 ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
     1501 agcgcgcaga gagggagtgg ccaactccat cactaggggt tcctgcggcc gcacgcgtta
     1561 cacactagtg cattagggaa ttcgatatca agcttatcga taatcaacct ctggattaca
     1621 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     1681 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     1741 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     1801 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     1861 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     1921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     1981 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     2041 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2101 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2161 gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta
     2221 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     2281 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     2341 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     2401 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     2461 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     2521 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     2581 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     2641 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     2701 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     2761 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     2821 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     2881 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     2941 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3001 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     3061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     3181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3241 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     3301 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     3361 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     3421 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     3481 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     3541 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3601 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3661 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3721 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3781 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3841 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3901 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3961 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4021 gcgcggtatt atcccgtatt gacgccgggc aagagcaact c
//
