LOCUS       sequence-417292-       12144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  episomal Cas9 and YUM1-targeting sgRNA expression vector for S. 
            stipitis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Reichard WD, Smith SE, Robertson JB
  TITLE     BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset 
            for repeatedly modifying wild-type Scheffersomyces stipitis.
  JOURNAL   mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. 
            Epub 2023 Jun 22.
  PUBMED    37345937
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2023-08-24"; volume: "8(4):e0022423. doi"; pages: " 
            10.1128/msphere.00224-23. Epub 2023 Jun 22"
COMMENT     Sequence Label: pWR63(YUM1)
FEATURES             Location/Qualifiers
     source          1..12144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             777..1805
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             7313..7333
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7337..7357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7361..7381
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7382..7447
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_RNA        8726..8801
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           8806..8873
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     rep_origin      complement(10389..10977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10469..10488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11148..11939,11940..12008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11771..11790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12009..12113)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatac cggtgcgatc gtgggcccta gtacttccct cgagccgtac acttattgtt
       61 aactatgaaa catatactta tggacaaggt tcactgattt ggactgaaaa gcaaaaacga
      121 gaaatgagtc aacgtctgag atcaatccct agaccacccg ggaaggtcat gtgtttcatg
      181 aagtacgata aggttggtaa ccgattgact cattggttcg tggcggagaa gtacgcagag
      241 taaaaccggg gccgattcgt ggtaaattct ggaatgatcc agaggcgcga catttatgca
      301 gacaatttgt gttttgtcgc aaacgatgtt atagcgaaat ttttcactct gtcagataaa
      361 tggattttgt caaaaggggg aagtagaagg agaatgggcc cgagatgttc tgccaaattc
      421 tcagtagcat aatgtgaaag aagcccttac attgtccagc ctctggcatc attaaaaacc
      481 gtagcggaaa ccaattgtct ctgttcttcc ctggcacacc ctggtagccc cacccagttg
      541 tagtacatct cacacgctgg caacttggga caatcagcaa cttttttttc ttttaatttt
      601 ttcagcgcga cattttgcct cttctgcgag aacagacttt ttcacctcca tctcaccccc
      661 ctttgcactt atataaattg gaccagttcc tcccattgta gaaaaaattt tgctggacct
      721 ttttctcttt tttttgtcct ttagtttcat acaatctaag tctatctaca tgtacaatgg
      781 gtaaaaagcc tgaactcacc gcgacctctg tcgagaagtt tttgatcgaa aagttcgaca
      841 gcgtctccga cttgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
      901 taggagggcg tggatatgtc ttgcgggtaa atagctgcgc cgatggtttc tacaaagatc
      961 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     1021 gggagttcag cgagagcttg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     1081 aagacttgcc tgaaaccgaa ttgcccgctg ttttgcagcc ggtcgcggag gccatggatg
     1141 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     1201 tcggtcaata cactacatgg cgtgatttca tttgcgcgat tgctgatccc catgtgtatc
     1261 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagt
     1321 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     1381 ccaacaatgt cttgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     1441 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     1501 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac
     1561 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     1621 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     1681 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     1741 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     1801 aataagcggc cgctaaccac ttgcaaaatc ctttgtattc ttgttctgca aacattttgt
     1861 ccaatctctt atctttctac gatgttgaag aacatacatt tttgttagtc cagcttgtta
     1921 tccttttttt attaaataaa aaattttatt gttaaagttg tttttcttct ttgtctgtcc
     1981 tgttttaact catatatatt ctatatatta cagaaagaaa gtagatcatc taaaataaac
     2041 atttatttga tgagacaaag taggtttttt agaatacaat gtagcatcct ctaatataca
     2101 atagagttgt tttataaaag cgatacattt aatattatga caggactggt gaagtgtata
     2161 gaattgtgct aatctggcaa acgaacccaa gaagacgtta aagcaaatag tgcattttgt
     2221 attacaatca agttcgtcga ccatgcatta gatcttgcat gcctctagaa cctaggtggc
     2281 gccacagctg tggatctcac aaagaaccaa caaactatgc attattcatt agccatggcc
     2341 ataatacatg gctgatggtt tcatctctat atttgttcta tatttaggcg tttgtgaatg
     2401 atatatacta atagacaata gaatatgacg acagaccatt tccaacaagg cgcaaagcac
     2461 caaggctcca aacgtgtgtg gcataaggtc ttccggaatg caccagatgc tgaagacttt
     2521 aatgtggcat ttttctttca aacttgagca acaagtttgc gtctgccaga acacgtaaga
     2581 aacgaataat ggtaatagat gagtctaccg tgtttgctag tatttacgct gtactattta
     2641 ctaataagta tcaggggctc ctcttgtgtc tagaagttat gaatgagttc ccatggctgt
     2701 gtgcctagat gcgctaccgc gttcagcctg aaattggtac tctaaaccta atacacgcct
     2761 ctcgagttta gacaaggtta tatttaatgg attcgcgcgt atgcctcaaa tttttttgat
     2821 ttgcattttt tgtttacgcg tttgttttga attcggtttg tttaccaaac agtgcgtacc
     2881 ttaatttttc tgatttgcgt agagatcgcc gagcacatag cgactcctta ccaggctcta
     2941 gaattatagc gtggatcctt cttatggtta tggactatta ttattttgat ttatggaagg
     3001 gggtctcgct tattgtcgtt tgccgtagca gggcacgtca aacgcgtcat aaaccagaat
     3061 ggttacaata tcgagagttg ggcagcgcca actgtctgcc tttgaggcca cctcaaacca
     3121 cactataaat aagggcagat gtaccctcaa tgcccaaaag atgaacactc tacttcttaa
     3181 ctcaaactag ggtaccatgg ataaaaagta tagtattggt ttagatattg gtactaactc
     3241 tgtgggttgg gcagttatca ctgacgaata taaagttcca tcaaagaaat ttaaggtgtt
     3301 aggtaacact gacagacact caataaaaaa gaatcttatc ggtgctcttt tgttcgactc
     3361 cggtgaaact gccgaggcta cacgtttaaa aagaacagca agaagaagat atacccgtag
     3421 aaaaaataga atatgttatt tacaagaaat cttttctaat gaaatggcta aagttgatga
     3481 ttcctttttc catagattgg aagagtcatt tttggttgaa gaagacaaaa agcatgagag
     3541 acatccaatc tttgggaata tagttgatga agtggcttac catgaaaaat atcctaccat
     3601 ttatcattta agaaagaaat tggtagattc aactgataaa gctgacctta gattaatcta
     3661 tttagcactt gcccatatga ttaaatttag aggtcatttt ttgattgaag gtgatttgaa
     3721 cccagataat tctgacgtgg ataaattatt tattcaatta gtccaaacct acaaccaatt
     3781 acttgaggaa aatccaatta atgctagtgg tgtcgatgcc aaagctatat tatcagccag
     3841 attatcaaaa tctagacgtt tggaaaattt gattgcccaa ttgccaggag aaaaaaagaa
     3901 tggattattt ggaaacttga tcgcattatc attgggtttg acaccaaatt ttaaatctaa
     3961 ttttgattta gctgaagatg ctaaattaca attatcaaaa gacacctatg acgacgattt
     4021 ggacaattta cttgctcaaa ttggtgatca atatgcagat ttgttcttag ctgctaaaaa
     4081 cttatctgat gctattttgt tgtctgatat tttgagagtg aacacagaaa taaccaaagc
     4141 tccattatca gcatctatga tcaaacgtta tgatgaacac catcaggatt tgactttatt
     4201 gaaagctttg gtgagacaac aattgccaga gaagtataaa gaaatctttt tcgatcaatc
     4261 taaaaacggg tatgcaggtt atattgatgg gggtgcctcc caagaggaat tttacaaatt
     4321 tataaaacct attttagaaa agatggatgg gactgaggaa cttttggtca aattgaacag
     4381 agaagatttg ttacgtaaac agagaacttt tgataatggt agtatacctc accaaattca
     4441 tttgggtgag ttgcatgcaa ttttaagaag acaagaagat ttttatccat ttttaaaaga
     4501 taatagagaa aaaatcgaga aaattttaac ctttagaatt ccatactatg ttgggccttt
     4561 ggctagaggt aattcaagat ttgcctggat gacacgtaaa tcagaagaaa ctattacccc
     4621 ttggaatttt gaagaggttg ttgataaagg agcatcagca cagagtttta ttgaaagaat
     4681 gaccaatttc gataaaaact taccaaatga aaaagtttta ccaaaacatt ccttgttata
     4741 cgaatatttt actgtttaca atgaacttac aaaggttaaa tatgttactg aaggtatgcg
     4801 taagccagcc tttttatctg gagaacagaa aaaggcaata gttgatttat tgtttaaaac
     4861 aaatagaaaa gttactgtta aacaattaaa agaagattac tttaagaaaa ttgaatgttt
     4921 tgattcagtt gaaatcagtg gtgttgaaga cagatttaat gctagtttag gagcttacca
     4981 tgatttactt aaaattatca aagataaaga tttcttggat aacgaagaaa atgaagacat
     5041 tttagaagac attgttttaa ccttaacttt attcgaagat agagagatga ttgaagaacg
     5101 tttgaagact tatgcacatt tgtttgacga taaagtgatg aaacagttga aaagaagacg
     5161 ttatactgga tggggtagat tgtctcgtaa attgatcaat ggaattagag ataaacaaag
     5221 tggtaaaact atcttggact ttttgaaatc tgacggattt gctaatagaa atttcatgca
     5281 attgatccac gacgatagtt tgacatttaa agaagacatc caaaaggccc aagtgagtgg
     5341 gcaaggtgat tcattacatg aacatattgc aaatttagcc ggatctcctg ctattaagaa
     5401 agggatatta caaactgtta aagttgtgga tgaattagtg aaagtaatgg gaagacataa
     5461 acctgaaaac attgtcattg agatggcaag agaaaatcaa actacacaaa aaggacagaa
     5521 aaatagtaga gaacgtatga aaagaataga agagggtatt aaagaattgg gtagtcagat
     5581 attgaaagaa cacccagtgg aaaataccca gttgcaaaat gaaaaattat atctttacta
     5641 ccttcaaaat ggacgtgata tgtatgttga tcaggaatta gatataaata gactttcaga
     5701 ttatgatgta gatcatatag ttccacaatc tttcttgaaa gatgattcca tagacaataa
     5761 agtattaact agaagtgata aaaatagagg taaaagtgat aatgtcccaa gtgaggaagt
     5821 cgtcaaaaag atgaaaaatt actggcgtca acttttgaat gctaaattaa ttactcaaag
     5881 aaaatttgat aatttgacta aagcagaaag aggtgggctt tctgaattag ataaagccgg
     5941 gttcattaaa agacaattgg tcgaaactag acaaattact aaacatgttg cccaaatttt
     6001 agattcccgt atgaacacta agtatgacga aaatgataag ttaatacgtg aggttaaagt
     6061 cattacttta aaatcaaaac ttgtctctga tttcagaaag gatttccaat tctataaagt
     6121 tagagaaatt aataattatc atcatgctca tgatgcatat ttgaatgctg tagttggaac
     6181 tgctttaatc aagaaatacc ctaaattaga atctgaattt gtatatggtg attacaaagt
     6241 ctatgatgtt agaaagatga ttgctaaatc agaacaagaa attggtaagg ctacagctaa
     6301 atacttcttt tactctaaca ttatgaattt ctttaaaaca gaaattactt tggcaaacgg
     6361 tgaaattaga aaaagacctc ttattgaaac aaatggtgag actggagaga tagtttggga
     6421 caaagggcgt gatttcgcta ctgttagaaa agttttatca atgccacaag ttaacattgt
     6481 aaagaaaaca gaggttcaaa ctggtggttt ctcaaaagaa agtattttgc ctaaaagaaa
     6541 tagtgataaa ttgattgcca gaaaaaagga ttgggatcca aagaaatatg gtggtttcga
     6601 ctcaccaacc ttagcctatt ctgttttggt tgtggcaaag gttgaaaagg gtaaaagtaa
     6661 aaagcttaaa tcagtaaaag aacttttggg tattacaata atggaaagaa gttcctttga
     6721 aaagaaccct attgattttt tggaagctaa aggttataag gaagtaaaga aggacttaat
     6781 aatcaaattg cctaaatatt ctttatttga attagaaaat gggagaaaaa gaatgttggc
     6841 ttctgctgga gaattgcaaa agggtaatga attagcattg ccttccaaat atgttgactt
     6901 cttgtattta gcttcacact atgaaaagtt gaaagggtca ccagaagata acgagcaaaa
     6961 acaattattt gttgaacaac acaaacacta cttagatgag attatagaac aaattagtga
     7021 attcagtaaa agagtgatat tagctgatgc aaatttagat aaagttttgt cagcctataa
     7081 caaacataga gataagccaa ttagagaaca agcagaaaac attattcact tatttaccct
     7141 taccaattta ggagcacctg ctgctttcaa gtattttgat acaacaattg atcgtaaaag
     7201 atatacctca acaaaagaag tcttagacgc caccttaatt catcaatcaa tcactggatt
     7261 gtatgagaca agaattgatt tgtctcaatt gggtggtggt gaaggggctg atcctaagaa
     7321 gaaaagaaaa gttgatccaa agaaaaagcg taaggtggat cctaagaaaa agagaaaggt
     7381 tgactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     7441 tgacaagtga taacttaagc attttagaca agtgcctatt tattcagatt gattttttat
     7501 attgaatatg gatgttgatt acgacttgtg ttacgaagca tgaacatgtg atttgaaaaa
     7561 gtgtttacga ataaaagaac tttttttgca accgttgcta gttgaagtgt agactctgaa
     7621 tgcgaagggt tgcaaatgtg cccagtagaa aaaatattcc atatttttct gaacttaatt
     7681 caatgaaata cgagtattac tacaactgcc aatatcggaa caatttaacg agagcaagct
     7741 atataaaatg aatgttcatt tacacaagct agaaccaaaa cttgaattaa aatttaacat
     7801 aactaaatgt aactagataa aagagagaca taaccaaacg ttcagaaggc agaagaattc
     7861 agttaactcc tgcaggctcg ttagtatttc cgtgaaggca gccattgtga aaattgaaca
     7921 tcacctgagg tattttagcc accagaagcg gcggtacgga agaaagtgtg tacgatggtt
     7981 ggtggtggaa ttgcgtgcat gcctgatggg gcaatattaa ttagatagag ctttggtgat
     8041 attagtggat aatagaattc acagagaaga catcaggagc aatttccaag agccattgat
     8101 gatgtaattg ccccaacagc aagattcaga tctgacaatt gaccaccgtt ttgtagaagc
     8161 aaaaaatcgt agattatcac caagagggtt tttcaccgaa ccagcaaata gaaactatgc
     8221 cgtagaactc gcccaggctt ttttgctagc actttccagc agtagaaccg tccaattaag
     8281 tcaacaggaa ccattgaggt cgagcccaac cacctgaacc ccctcacggt cgtgtcccta
     8341 ttattgatcc agagggtgcc agtttcggta gccaatattg gttcatgggt ttctatggcc
     8401 cggagtgagt ttgcaggttg gccccggcgc cgtctgcagg atgggagtta tagcggccaa
     8461 acttcacatt tcgaaatcct gctgcagcca atctgaagaa ttaatataaa ttcgtgtcga
     8521 atcgccgtct gtgaaatttc agtacttgat tttcttttct tcttcttttt ctcttttgtt
     8581 tcttcagaat caatacacat tttttcttcc ctataaacaa ttcatcggaa acgggcgtgt
     8641 ggcgtagttg gtagcgcgtt cgcttagcat gcgaaaggtc tccggttcga ctccggactc
     8701 gtccaaaagt tctacttagt ccccggtttt agagctagaa atagcaagtt aaaataaggc
     8761 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttggccg gcatggtccc
     8821 agcctcctcg ctggcgccgg ctgggcaaca tgcttcggca tggcgaatgg gacacgcgtg
     8881 ctagatagtg ctttgttcta ttccacctcg tgttttattt accttttaac tagtgctaat
     8941 ttacgaataa tgtacgttag aaaaattgac ctgtttatat atgggtgcaa aaaagttgcg
     9001 tcctgtccaa aggttgcatt tcccctcaag caaccaaaag gagttaggat ctgggaacta
     9061 tttaacgccg ctggggcaac aattgagatg tcgtagacgc catgccccac ctaaccaatg
     9121 cactctccgc ggcttaatta aagctagcca gcgcttgagc tctctgcggt gtctacaagg
     9181 tcagaagtat tttaactcat ccaaaagccc cagacaccgc attatttccc ggtgcatgca
     9241 ttcttctctg acaagtacct tctctctcca ttggctggat aaaagaatct gtaggtcgcc
     9301 gacatcttgt ttgtgtctac atggccaaca tacttctctt acaatgtatg tgcatatgca
     9361 tatgtaagtg gagcgtggtg ggaggcttta ttgtagagct attgttctga cggagtaggt
     9421 gcaaacggag tctgtttagg tacagtcacc atctctgcgt atctgtacat tttcactgga
     9481 gctagaagga gctactcttc gacttcgaga acaggggagc aacccagttt gtgttcaagg
     9541 cgattgttag ttgctccggc aagataaggt tacattattc tctacgcttt agcgtttagg
     9601 ggtactcgga taaatgccag atgaaaaacg ctaggtgcat acagtacaag tcgagccgac
     9661 attgttggac cgccccgata attattgtat gttgtgtttg cttgtaagac catgattgat
     9721 atttattggg tggttcagca atcggactcg ttgattatta ttattattga ccctcatgat
     9781 atacaatgga ttttatttct gagcaatcgt agcaaaatat tcgacgcaat gtagcttgag
     9841 tttatgacct tagcggacat caccagcgca gctttacatt ttccattctt tggatttatt
     9901 tttcttatcc actaggagat tagaagtcta agtatcatgt aagaaaggag ataattgttg
     9961 tctgctggat aataaaattt tgctgagtta gaaatgagat acgaatcagc aagatgtctt
    10021 tggttgcagc ctgtagagat ttagatttcg acaatctgac taaaatgtac gaagagaaaa
    10081 atagacacat ttagtcaata cttcagcgtt aagggtgcta tagactgtgc aacttcgttt
    10141 cactgctgac atgaaattcc agagacttaa catattacat gattataaat aatttgcgta
    10201 taatatgttt ggcataaagc gatttctgga aaagttgcga aaaattccga attactacaa
    10261 cttaccaaaa aaacagaggc atattttgct aaatcaccat atcctatact gagctccatc
    10321 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    10381 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    10441 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    10501 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    10561 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    10621 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    10681 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    10741 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    10801 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    10861 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    10921 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10981 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11041 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11101 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11161 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11221 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    11281 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    11341 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    11401 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    11461 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    11521 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    11581 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    11641 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    11701 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    11761 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    11821 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    11881 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11941 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12001 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12061 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12121 ccccgaaaag tgccacctga cgtc
//
