LOCUS       sequence-417296-        3812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_153 [pCB102 ORI]
FEATURES             Location/Qualifiers
     source          1..3812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(136..152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(136..152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(149..171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(324..912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(404..423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1045..1139)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1160..1819)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1734..1753
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1820..1922)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2009..2031
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2023..2040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2024..2040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaccacccca ggatacatag attaccacaa ctccgagccc ttccacctag cgtgctaacc
       61 acgcacgcta ttgttgtata ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
      121 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
      181 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
      241 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      721 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
      961 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cccccatacg
     1021 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1081 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1141 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1201 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1261 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1321 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1381 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1441 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1501 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1561 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1621 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1681 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1741 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1801 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1861 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     1921 caacgtctca ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     1981 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     2041 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac cttagcgtgc
     2101 gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc tcgtctcgtc
     2161 tccgaggtct ccggagggcg cgccgccatt atttttttga acaattgaca attcatttct
     2221 tattttttat taagtgatag tcaaaaggca taacagtgct gaatagaaag aaatttacag
     2281 aaaagaaaat tatagaattt agtatgatta attatactca tttatgaatg tttaattgaa
     2341 tacaaaaaaa aatacttgtt atgtattcaa ttacgggtta aaatatagac aagttgaaaa
     2401 atttaataaa aaaataagtc ctcagctctt atatattaag ctaccaactt agtatataag
     2461 ccaaaactta aatgtgctac caacacatca agccgttaga gaactctatc tatagcaata
     2521 tttcaaatgt accgacatac aagagaaaca ttaactatat atattcaatt tatgagatta
     2581 tcttaacaga tataaatgta aattgcaata agtaagattt agaagtttat agcctttgtg
     2641 tattggaagc agtacgcaaa ggctttttta tttgataaaa attagaagta tatttatttt
     2701 ttcataatta atttatgaaa atgaaagggg gtgagcaaag tgacagagga aagcagtatc
     2761 ttatcaaata acaaggtatt agcaatatca ttattgactt tagcagtaaa cattatgact
     2821 tttatagtgc ttgtagctaa gtagtacgaa agggggagct ttaaaaagct ccttggaata
     2881 catagaattc ataaattaat ttatgaaaag aagggcgtat atgaaaactt gtaaaaattg
     2941 caaagagttt attaaagata ctgaaatatg caaaatacat tcgttgatga ttcatgataa
     3001 aacagtagca acctattgca gtaaatacaa tgagtcaaga tgtttacata aagggaaagt
     3061 ccaatgtatt aattgttcaa agatgaaccg atatggatgg tgtgccataa aaatgagatg
     3121 ttttacagag gaagaacaga aaaaagaacg tacatgcatt aaatattatg caaggagctt
     3181 taaaaaagct catgtaaaga agagtaaaaa gaaaaaataa tttatttatt aatttaatat
     3241 tgagagtgcc gacacagtat gcactaaaaa atatatctgt ggtgtagtga gccgatacaa
     3301 aaggatagtc actcgcattt tcataataca tcttatgtta tgattatgtg tcggtgggac
     3361 ttcacgacga aaacccacaa taaaaaaaga gttcggggta gggttaagca tagttgaggc
     3421 aactaaacaa tcaagctagg atatgcagta gcagaccgta aggtcgttgt ttaggtgtgt
     3481 tgtaatacat acgctattaa gatgtaaaaa tacggatacc aatgaaggga aaagtataat
     3541 ttttggatgt agtttgtttg ttcatctatg ggcaaactac gtccaaagcc gtttccaaat
     3601 ctgctaaaaa gtatatcctt tctaaaatca aagtcaagta tgaaatcata aataaagttt
     3661 aattttgaag ttattatgat attatgtttt tctattaaaa taaattaagt atatagaata
     3721 gtttaataat agtatatact taatgtgata agtgtctgac agtgtcacag aaaggatgat
     3781 tgttatggat tataagcggc cggcccgctc ga
//
