LOCUS       sequence-417298-        5633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_154 [pCD6 ORI]
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(136..152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(136..152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(149..171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(324..912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(404..423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1045..1139)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1160..1819)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1734..1753
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1820..1922)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2009..2031
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2023..2040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2024..2040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaccacccca ggatacatag attaccacaa ctccgagccc ttccacctag cgtgctaacc
       61 acgcacgcta ttgttgtata ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
      121 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
      181 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
      241 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      721 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
      961 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cccccatacg
     1021 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1081 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1141 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1201 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1261 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1321 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1381 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1441 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1501 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1561 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1621 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1681 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1741 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1801 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1861 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     1921 caacgtctca ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     1981 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     2041 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac cttagcgtgc
     2101 gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc tcgtctcgtc
     2161 tccgaggtct ccggagggcg cgcccgccct taagtctaaa aattagggga gatgtaagga
     2221 tttgggaaaa atagaagatg ttataatcat aaatatggta ttcgtaggct taaagtcaaa
     2281 aaggaggtga aatataaata gatttttagc taaattaagt aagaaatagg aggagattta
     2341 ttgaacaaaa aattagaaaa accatttgta tataagagag agtacgattt gactggatat
     2401 gatgttgaaa ttttacaaaa atatgagtta gaacaagcaa tatatgttta tgttgggagt
     2461 agttgtgcat ataacatgag agctagaagt agtaaatgga gataccatat aagaacaaat
     2521 aataagtcta tatgttgtaa cattaaaaat tttatacata acttggaatt gttttataaa
     2581 atggaattaa agttgtcaga taatattatt aatgataagc tatactatag caatatagca
     2641 gagtttgaag aatttgaaac actagaaaaa gctagagagg tagaaagtac tattataagt
     2701 caatatcaat ttttagattc tataaatcac atgttaaaac aaaaaataat tttattgagt
     2761 aataaggata gtgtgttaaa cataactaaa aatggaaata caaattattt gaaagtaaaa
     2821 aataaataca tagaaaaaca taagaacaag ccaataatga gataccatat caactgtcaa
     2881 ttcaatacag atggaagtgt caaaagtatt acacaggagt ttgaaccaat attggaatta
     2941 aacaaaaaaa ataccctaag ccgaccaagc agagtatttt taaaataata ttttaagata
     3001 acaacaaaat gagataatac tactagacaa tgacaactca actaccaatt gagtttatgg
     3061 agctaccaac tccaatatcg gtctaactga ttaagtatct gtagttatat aataatattg
     3121 ctatcaattt tagcatctta acaatattat tatacatact aagctaaaat tattcaatag
     3181 ttgtaaaagt tgattagtca ataagtatat atttaatgta gtgttatctc ttaaaaaaac
     3241 tagataagga gataataaat atatggaaca attagattca aaatataagt tgaaaaaatt
     3301 tctaatggca gtatttagag atggtatagg acaaggaaat aatcttattg ataatgaata
     3361 tgttagagta tttcaaaata ataaaagtaa tagtaaacaa ttagaactcg gagaagaatt
     3421 taaagaatat agtaaaacaa ctttttttaa aaatatagat gatatagtag aatttacctt
     3481 cgcaaaaaat atttattatg aaaatacatt ttttaaccta tgtactactg atggaaaagc
     3541 aggaaccaat gaaaacttaa taaatagata tgcattagga tttgattttg acaaaaaaga
     3601 attaggacaa ggttttaatt ataaagatat aattaattta tttactaaga taggattaca
     3661 ttatcatatc ctagttgata gtggaaatgg attccatgtt tatgtgctaa ttaataaaac
     3721 taataacatt aagttagtat cagaagttac aaatacatta ataaataaat tgggtgcaga
     3781 taaacaagca aatttatcta ctcaagtatt aagagtacct tatacatata atattaaaaa
     3841 tactactaaa caagtaaaaa taatacacca agacaaaaat atatatagat atgacataga
     3901 aaagttagct aaaaaatatt gcaaagatgt aaaaacagta ggtaatacta atacaaaata
     3961 tatattagat agtaagctac caaattgtat agtagatatt ttaaaaaatg gtagtaaaga
     4021 tggacataaa aacctagatt tgcaaaaaat agttgtgact ttaagattga ggaataaaag
     4081 tttaagtcaa gtaatatccg ttgctagaga atggaactat atatcacaaa atagtctttc
     4141 aaatagtgag ctagaatatc aagtcaagta tatgtatgag aaacttaaaa cggttaattt
     4201 tggttgtact ggttgtgagt ttaatagtga ttgttggaat aaaatagaat cagattttat
     4261 atatagtgat gaagatactt tgttcaatat gccacataag cactcaaagg atttgaaata
     4321 taagaatagg aaaggggtta aaataatgac tggtaatcaa ttgtttatct ataatgtgtt
     4381 acttaacaat aaagatagag aattaaacat agacgatata atggagctga taacctataa
     4441 acgtaagaag aaagttaaaa acattgttat gagtgaaaag acattaagag aaacattaaa
     4501 agaacttcaa cataatgatt atattacaaa aacaaaaggt gttacaaagc taggaataaa
     4561 agatacatac aatgtaaaag aagttagatg taatatagat aaacaatata ctattagtta
     4621 ctttgttacc atggcagtaa tttggggaat aatttcaact gaagaattaa gattatatac
     4681 tcacatgaga tataagcaag atttattggt caaagatgat aaaataaaag gaaatatatt
     4741 aagaattaat caagaggaat tagcaaaaga tttaggagta acacagcaaa gaatttcaaa
     4801 tatgatagaa tctttattag atactaaaat tttagatgta tgggaaacta aaataaatga
     4861 tagaggattt atgtactata catatagatt aaacaagtag atttttgata ggattagaat
     4921 tgattttcta gtcctatttt tatgcaaaaa aactaattaa taaaaatttc ttttggtaaa
     4981 ataattgtac gagaattgca aaaaaaaaat ggcatcaaag tattgaaatt aagccgtttt
     5041 aaaaatttct tttggtaaaa taattctaca tatatatgta gtatatatat atatgttttt
     5101 tagagaatgt ataactagaa tatagagcta gaatatagag aatgtataac tagaatatag
     5161 agctagaata tagagaatgt ataactagaa tatagagcta gaatatagag aatgtataac
     5221 tagaatatag agctagaata tagagaatgt ataactagaa tatagagcta gaatatagag
     5281 aatgtataac tagaatatag agctagaata tagagaatgt ataactagaa tatagagcta
     5341 gaatcctaat tagtaggtgc ttttttaaaa caagttaaaa atcaaaaata gtattagtaa
     5401 gcattggaaa tgctagattc taaaatagaa aagtaaaaaa ttggtgcact atctaaactt
     5461 atctatatcg ctttttccgt cgtttggttc tctagttacg atacagggga tatgcttata
     5521 ttgagttata gtactaatca gtgcttaata tagttaataa aattatagtt accatagttt
     5581 agtaactatg atgtatgtta gttagaaact tgcatttcgg ccggcccgct cga
//
