LOCUS       sequence-417299-        3065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_155 [pIM13 ORI]
FEATURES             Location/Qualifiers
     source          1..3065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(343..437)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(458..1117)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1032..1051
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1118..1220)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1307..1329
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1321..1338
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1322..1338
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2499..2515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2499..2515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2512..2534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(2687..3065,1..210))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2767..2786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
       61 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      241 gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc
      301 aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac caataaaaaa
      361 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
      421 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
      481 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
      541 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
      601 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
      661 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
      721 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
      781 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
      841 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
      901 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
      961 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1021 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1081 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1141 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1201 aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca tcgtacggcc
     1261 aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt
     1321 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
     1381 cgcctgacct tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc
     1441 aggctgtctc gtctcgtctc cgaggtctcc ggagggcgcg ccgcattcac ttcttttcta
     1501 tataaatatg agcgaagcga ataagcgtcg gaaaagcagc aaaaagtttc ctttttgctg
     1561 ttggagcatg ggggttcagg gggtgcagta tctgacgtca atgccgagcg aaagcgagcc
     1621 gaagggtagc atttacgtta gataaccccc tgatatgctc cgacgcttta tatagaaaag
     1681 aagattcaac taggtaaaat cttaatatag gttgagatga taaggtttat aaggaatttg
     1741 tttgttctaa tttttcactc attttgttct aatttctttt aacaaatgtt cttttttttt
     1801 tagaacagtt atgatatagt tagaatagtt taaaataagg agtgagaaaa agatgaaaga
     1861 aagatatgga acagtctata aaggctctca gaggctcata gacgaagaaa gtggagaagt
     1921 catagaggta gacaagttat accgtaaaca aacgtctggt aacttcgtaa aggcatatat
     1981 agtgcaatta ataagtatgt tagatatgat tggcggaaaa aaacttaaaa tcgttaacta
     2041 tatcctagat aatgtccact taagtaacaa tacaatgata gctacaacaa gagaaatagc
     2101 aaaagctaca ggaacaagtc tacaaacagt aataacaaca cttaaaatct tagaagaagg
     2161 aaatattata aaaagaaaaa ctggagtatt aatgttaaac cctgaactac taatgagagg
     2221 cgacgaccaa aaacaaaaat acctcttact cgaatttggg aactttgagc aagaggcaaa
     2281 tgaaatagat tgacctccca ataacaccac gtagttattg ggaggtcaat ctatgaaatg
     2341 cgattaaggg ccggcccgct cgagaccacc ccaggataca tagattacca caactccgag
     2401 cccttccacc tagcgtgcta accacgcacg ctattgttgt ataggctagg tggaggctca
     2461 gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt catagctgtt tcctgtgtga
     2521 aattgttatc cgctcagagg gcacaatcct attccgcgct atccgacaat ctccaagaca
     2581 ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag
     2641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3061 gagcg
//
