LOCUS       sequence-417307-        3689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_160 [ermB]
FEATURES             Location/Qualifiers
     source          1..3689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1436..1452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1436..1452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1449..1471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1460..1476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1484..1490,1491..1508,1509..1514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1529..1550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1667..1684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1838..2426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1918..1937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2597..3388,3389..3457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3220..3239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3458..3562)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3630..3648
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccc tagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga caggcgacag
      481 attagcgtgc gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc
      541 tcgtctcgtc tccgaggtct caaatgaaca aaaatataaa atattctcaa aactttttaa
      601 cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg
      661 tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac
      721 aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac
      781 tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac
      841 agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag
      901 tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc
      961 gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc
     1021 aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa
     1081 taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact
     1141 ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc
     1201 aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt
     1261 ctatttttaa tagttatcta ttatttaacg ggaggaaata agctttgaga ccaccccagg
     1321 atacatagat taccacaact ccgagccctt ccacctagcg tgctaaccac gcacgctatt
     1381 gttgtatatc agttctggac cagcgagctg tgctgcgact cgtggcgtaa tcatggtcat
     1441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgtcgc
     1981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2701 gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca ccggctccag
     2761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3421 agggaataag ggcgacacgg aaatgttgaa tactcatact ctaccttttt caatattatt
     3481 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3541 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3601 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcatctcg
     3661 cgcgtttcgg tgcacgaggc cctttcatc
//
