LOCUS       sequence-417310-        3707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_165 [aad(9)]
FEATURES             Location/Qualifiers
     source          1..3707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1454..1470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1454..1470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1467..1489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1478..1494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1502..1508,1509..1526,1527..1532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1547..1568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1685..1702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1856..2444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1936..1955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2615..3406,3407..3475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3238..3257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3476..3580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3648..3666
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccc tagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga caggcgacag
      481 attagcgtgc gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc
      541 tcgtctcgtc tccgaggtct caaatgttga atacatacga acaaattaat aaagtgaaaa
      601 aaatacttcg gaaacattta aaaaataacc ttattggtac ttacatgttt ggatcaggag
      661 ttgagagtgg actaaaacca aatagtgatc ttgacttttt agtcgtcgta tctgaaccat
      721 tgacagatca aagtaaagaa atacttatac aaaaaattag acctatttca aagaaaatag
      781 gagataaaag caacttacga tatattgaat taacaattat tattcagcaa gaaatggtac
      841 cgtggaatca tcctcccaaa caagaattta tttatggaga atggttacaa gagctttatg
      901 aacaaggata cattcctcag aaggaattaa attcagattt aaccataatg ctttaccaag
      961 caaaacgaaa aaataaaaga atatacggaa attatgactt agaggaatta ctacctgata
     1021 ttccattttc tgatgtgaga cgcgccatta tggattcgtc agaggaatta atagataatt
     1081 atcaggatga tgaaaccaac tctatattaa ctttatgccg tatgatttta actatggaca
     1141 cgggtaaaat cataccaaaa gatattgcgg gaaatgcagt ggctgaatct tctccattag
     1201 aacataggga gagaattttg ttagcagttc gtagttatct tggagagaat attgaatgga
     1261 ctaatgaaaa tgtaaattta actataaact atttaaataa cagattaaaa aaattataag
     1321 ctttgagacc accccaggat acatagatta ccacaactcc gagcccttcc acctagcgtg
     1381 ctaaccacgc acgctattgt tgtatatcag ttctggacca gcgagctgtg ctgcgactcg
     1441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1981 ctgttccgac cctgtcgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2281 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagatc
     2761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3481 acctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3601 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     3661 cgaggccctt tcatctcgcg cgtttcggtg cacgaggccc tttcatc
//
