LOCUS       sequence-417318-       10209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty BLINCAR plasmid for payload delivery, no payload, integrates 
            at EGC3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Reichard WD, Smith SE, Robertson JB
  TITLE     BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset 
            for repeatedly modifying wild-type Scheffersomyces stipitis.
  JOURNAL   mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. 
            Epub 2023 Jun 22.
  PUBMED    37345937
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2023-08-24"; volume: "8(4):e0022423. doi"; pages: " 
            10.1128/msphere.00224-23. Epub 2023 Jun 22"
COMMENT     Sequence Label: pWR89
FEATURES             Location/Qualifiers
     source          1..10209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1443..2252
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3448..5076
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG99luc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     RBS             4398..4406
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(5527..5543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(8454..9042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8534..8553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9213..10004,10005..10073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9836..9855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10074..10178)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatac cggtgcgatc gtgggcccta gtacttccct cgaccatgca ttagatcttg
       61 catgcctcta gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg
      121 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      181 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      241 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
      301 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
      361 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
      421 agaatccgac gggttgttac tcgctcacac ttaatgttga tgaaagctgg ctacaggaag
      481 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
      541 gctgggtcag ttaaggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt
      601 tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc
      661 tggaagatca ggatatgtgg cggataaagt tctacttagt ccccggggat ccgtacactt
      721 attgttaact atgaaacata tacttatgga caaggttcac tgatttggac tgaaaagcaa
      781 aaacgagaaa tgagtcaacg tctgagatca atccctagac cacccgggaa ggtcatgtgt
      841 ttcatgaagt acgataaggt tggtaaccga ttgactcatt ggttcgtggc ggagaagtac
      901 gcagagtaaa accggggccg attcgtggta aattctggaa tgatccagag gcgcgacatt
      961 tatgcagaca atttgtgttt tgtcgcaaac gatgttatag cgaaattttt cactctgtca
     1021 gataaatgga ttttgtcaaa agggggaagt agaaggagaa tgggcccgag atgttctgcc
     1081 aaattctcag tagcataatg tgaaagaagc ccttacattg tccagcctct ggcatcatta
     1141 aaaaccgtag cggaaaccaa ttgtctctgt tcttccctgg cacaccctgg tagccccacc
     1201 cagttgtagt acatctcaca cgctggcaac ttgggacaat cagcaacttt tttttctttt
     1261 aattttttca gcgcgacatt ttgcctcttc tgcgagaaca gactttttca cctccatctc
     1321 accccccttt gcacttatat aaattggacc agttcctccc attgtagaaa aaattttgct
     1381 ggaccttttt ctcttttttt tgtcctttag tttcatacaa tctaagtcta tctacatgta
     1441 caatgggtaa ggaaaagact cacgtttcga ggccgagatt aaattccaac atggatgctg
     1501 atttatatgg gtataaatgg gctagagata atgtcgggca atcaggtgcg acaatctata
     1561 gattgtatgg gaagcccgat gcgccagagt tgtttttgaa acatggcaaa ggtagcgttg
     1621 ccaatgatgt tacagatgag atggtcagac taaactggtt gacggaattt atgcctcttc
     1681 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     1741 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     1801 atgcgttggc agtgttcttg agaagattgc attcgattcc tgtttgtaat tgtcctttta
     1861 acagcgatag agtatttcgt ctcgctcagg cgcaatcaag aatgaataac ggtttggttg
     1921 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1981 tgcataagtt gttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2041 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga agagtcggaa
     2101 tcgcagacag ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2161 cattacagaa aagacttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2221 agtttcattt gatgctcgat gagtttttct aagcggccgc taaccacttg caaaatcctt
     2281 tgtattcttg ttctgcaaac attttgtcca atctcttatc tttctacgat gttgaagaac
     2341 atacattttt gttagtccag cttgttatcc tttttttatt aaataaaaaa ttttattgtt
     2401 aaagttgttt ttcttctttg tctgtcctgt tttaactcat atatattcta tatattacag
     2461 aaagaaagta gatcatctaa aataaacatt tatttgatga gacaaagtag gttttttaga
     2521 atacaatgta gcatcctcta atatacaata gagttgtttt ataaaagcga tacatttaat
     2581 attatgacag gactggtgaa gtgtatagaa ttgtgctaat ctggcaaacg aacccaagaa
     2641 gacgttaaag caaatagtgc attttgtatt acaatcaagt tcgtcgagat cctcgttagt
     2701 atttccgtga aggcagccat tgtgaaaatt gaacatcacc tgaggtattt tagccaccag
     2761 aagcggcggt acggaagaaa gtgtgtacga tggttggtgg tggaattgcg tgcatgcctg
     2821 atggggcaat attaattaga tagagctttg gtgatattag tggataatag aattcacaga
     2881 gaagacatca ggagcaattt ccaagagcca ttgatgatgt aattgcccca acagcaagat
     2941 tcagatctga caattgacca ccgttttgta gaagcaaaaa atcgtagatt atcaccaaga
     3001 gggtttttca ccgaaccagc aaatagaaac tatgccgtag aactcgccca ggcttttttg
     3061 ctagcacttt ccagcagtag aaccgtccaa ttaagtcaac aggaaccatt gaggtcgagc
     3121 ccaaccacct gaaccccctc acggtcgtgt ccctattatt gatccagagg gtgccagttt
     3181 cggtagccaa tattggttca tgggtttcta tggcccggag tgagtttgca ggttggcccc
     3241 ggcgccgtct gcaggatggg agttatagcg gccaaacttc acatttcgaa atcctgctgc
     3301 agccaatctg aagaattaat ataaattcgt gtcgaatcgc cgtctgtgaa atttcagtac
     3361 ttgattttct tttcttcttc tttttctctt ttgtttcttc agaatcaata cacatttttt
     3421 cttccctata aacaattcat cggtaccatg gtgaagcgtg agaaaaatgt catctatggc
     3481 cctgagcctc tccatccttt ggaggatttg actgccggcg aaatgttgtt tcgtgctctc
     3541 cgcaagcact ctcatttgcc tcaagccttg gtcgatgtgg tcggcgatga atctttgagc
     3601 tacaaggagt tttttgaggc aaccgtcttg ttggctcagt ccctccacaa ttgtggctac
     3661 aagatgaacg acgtcgttag tatctgtgct gaaaacaata cccgtttctt cattccagtc
     3721 atcgccgcat ggtatatcgg tatgatcgtg gctccagtca acgagagcta cattcccgac
     3781 gaattgtgta aagtcatggg tatctctaag ccacagattg tcttcaccac taagaatatt
     3841 ttgaacaaag tcttggaagt ccaaagccgc accaacttta ttaagcgtat catcatcttg
     3901 gacactgtgg agaatattca cggttgcgaa tctttgccta atttcatctc tcgctattca
     3961 gacggcaaca tcgcaaactt taaaccactc cacttcgacc ctgtggaaca agttgcagcc
     4021 attttgtgta gcagcggtac tactggactc ccaaagggag tcatgcagac ccatcaaaac
     4081 atttgcgtgc gtttgatcca tgctctcgat ccacgtgtgg gcactcagtt gattcctggt
     4141 gtcaccgtct tggtctactt gcctttcttc catgctttcg gctttagcat tactttgggt
     4201 tactttatgg tcggtctccg cgtgattatg ttccgccgtt ttgatcagga ggctttcttg
     4261 aaagccatcc aagattatga agtccgcagt gtcatcaacg tgcctagcgt gatcttgttt
     4321 ttgtctaaga gcccactcgt ggacaagtac gacttgtctt cattgcgtga attgtgttgc
     4381 ggtgccgctc cattggctaa ggaggtcgct gaagtggccg ccaaacgctt gaatcttcca
     4441 ggtattcgtt gtggcttcgg cctcaccgaa tctaccagcg ctaacattca ctctctcggt
     4501 gatgagttta agagcggctc tttgggccgt gtcactccac tcatggctgc taagatcgct
     4561 gatcgcgaaa ctggtaaggc tttgggtccg aaccaagtgg gcgagttgtg tatcaaaggc
     4621 cctatggtga gcaagggtta tgtcaataac gttgaagcta ccaaggaggc catcgacgac
     4681 gacggctggt tgcattctgg tgattttgga tattacgacg aagatgagca tttttacgtc
     4741 gtggatcgtt acaaggagtt gatcaaatac aagggtagcc aggttgctcc agctgagttg
     4801 gaggagattt tgttgaaaaa tccatgcatt cgcgatgtcg ctgtggtcgg cattcctgat
     4861 ttggaggccg gcgaattgcc ttctgctttc gttgtcaagc agcctggtaa agaaattacc
     4921 gccaaagaag tgtatgatta cttggctgaa cgtgtgagcc atactaagta cttgcgtggc
     4981 ggcgtgcgtt ttgttgactc catccctcgt aacgtaacag gcaaaattac ccgcaaggag
     5041 ttgttgaaac aattgttgga gaaggccggc ggttagctta attaagcatt ttagacaagt
     5101 gcctatttat tcagattgat tttttatatt gaatatggat gttgattacg acttgtgtta
     5161 cgaagcatga acatgtgatt tgaaaaagtg tttacgaata aaagaacttt ttttgcaacc
     5221 gttgctagtt gaagtgtaga ctctgaatgc gaagggttgc aaatgtgccc agtagaaaaa
     5281 atattccata tttttctgaa cttaattcaa tgaaatacga gtattactac aactgccaat
     5341 atcggaacaa tttaacgaga gcaagctata taaaatgaat gttcatttac acaagctaga
     5401 accaaaactt gaattaaaat ttaacataac taaatgtaac tagataaaag agagacataa
     5461 ccaaacgttc agaaggcaga actagtaaag ttctacttag tccccgggga ccatgattac
     5521 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5581 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5641 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
     5701 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
     5761 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac
     5821 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
     5881 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
     5941 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcagtt aaggccagga
     6001 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
     6061 cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg
     6121 gatcttaagg tggccaactc atatggcgta cgagaattca gttaactcct gcaggaatcg
     6181 attggccggc ctcacgtgga cgcgttatta atcgtttaaa cctacgtaac cgcggcttaa
     6241 ttaaagctag ccagcgcttg agctcagctc aatccgtacc acatgctctt catgaatagc
     6301 ggccccagcc gtgcctgatc catgcgtcaa ctgaatttgc cgtgacatca ccacatttta
     6361 cagaacttga acgttaatct cagagtaata gagccattct tgggatgtag ttgatcggct
     6421 tgtggttaat tatactacat tattgtaatt tggagataat actgtctgga cctctaacaa
     6481 tccgaggtgc agaaaatgaa gaactcttct ttatgcacct tcttccttcg ctgaagagct
     6541 ttttgactcc acttttccgt tcgaaaatcc ggggtaggaa ccctttccta ttttatctgg
     6601 agttggaaca ctttcctatt tcatctggag ttggaacact ttcctatttc atctcgaagt
     6661 tctccattag gaaatggaaa tactgttcat ccgagaatct tgacacttga tagattcaca
     6721 gtgacaattc tagaaagcag ctgtaatcgg aaaagcacct ccacttttct gataacggag
     6781 ttcggctcaa tgttctattc ccccgacaag ccacgtcctt ttctctatat ttgtaatcag
     6841 gaaacaagaa gctacaaccc ggatcgtaga taagtagtga tatttagttg gcatcttttg
     6901 aattatgatt ttttgtaatt acgaattaac tcacaaacat gtctgccggt ttcttgacca
     6961 ctgcaggtac gaagatcgtt gatgctgaag gaaccccggt cgtccttaaa ggggcagctt
     7021 tgggcgggca cttgaatatg gagaacttta ttactggtta tcccggtcat gaaaccgaac
     7081 ataagttggt cttggagaaa aaaataggta aagagaagtt cgactatttt ttcgaaaagt
     7141 tctacgaata tttctggact gagaaggatg ctgaattcta cagaaataaa ttgggtttta
     7201 actgtttgag aattcctttc aattatcgac acttcatcga cgataatggt gatttgttca
     7261 aaattaaggg aaagggcttt gaattgttgg atagaatagt agatatctgt tcccagtacg
     7321 gaatctatac tattttggat ttacacacaa ctcctggtgg acagaaccaa ggttggcact
     7381 ctgattctgc tattcacaag tctctctttt gggatttcaa ggttttccaa gattcaattg
     7441 ttaacctttg ggttgagttg gccaagcatt acaaagacaa tgtctgggtt actggttaca
     7501 atccattgaa cgagcctgcc gtttcagact ctgaaaagtt ggtcgacttc tacaaaagat
     7561 tgcacgacga agttagaccc attgatccca accacatttt cttccttgat ggaaacacat
     7621 atgcaatgga cttcaggaaa ttcccttcgc cagaatccta tattcctaat acagtatatt
     7681 caattcatga ttactctacc tatggtttcc caaatcttga aggtgcatta tacactggtt
     7741 cagaagagga aaagtcaaaa ttaaaatctc aatataacag aaagatcgag taccaaagtg
     7801 aatacaaagt tcctgtttgg aatggtgagt ttggacccgt ttatgcttca aaggaaagag
     7861 gtgacaaaaa tccggaagta atcaaccggg cacggttcaa tgtcttgaaa gaccaattag
     7921 aagtctacag aaagggagat ccatcaggtg acggctcccc tatttcgtgg tcaatttggt
     7981 tgtacaaaga tattggtttc caaggtttga cttacgtctc tcccaagtca aaatggtatg
     8041 aggtatttgg agaatggcta cttaagaaga agaagttggg tttagataaa tggggcaatg
     8101 acattgaccc gggttataat caattgtacc aaaacttggt agaccatatg gaagccaatg
     8161 tcccagaaaa gtatcataaa gttctatacc ctcatacatg gacaatggag aaatatttgg
     8221 cccgtgtttc tagagatatg ctcttttcac aatacgctca acatgaatat gctgatttgt
     8281 tcgttggatt ttctttagaa gaacttgacg aattagccgc ttctttcaaa tttgagaatc
     8341 tagatcaaag agaggaattg aatcagatat tgaaagagct ccatcggaaa gaacatgtga
     8401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10201 cctgacgtc
//
