LOCUS       sequence-417344-       10880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized cspB from Clavibacter michiganensis in a 
            broad host range vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Stevens DM, Moreno-Perez A, Weisberg AJ, Ramsing C, Fliegmann J, 
            Zhang N, Madrigal M, Martin G, Steinbrenner A, Felix G, Coaker G
  TITLE     Natural variation of immune epitopes reveals intrabacterial 
            antagonism.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jun 4;121(23):e2319499121. doi: 
            10.1073/pnas.2319499121. Epub 2024 May 30.
  PUBMED    38814867
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-06-4"; volume: "121(23):e2319499121. 
            doi"; pages: " 10.1073/pnas.2319499121. Epub 2024 May 30"
COMMENT     Sequence Label: pDSK519-cspB
FEATURES             Location/Qualifiers
     source          1..10880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      243..637
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            979..1066
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2305..3276
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3787..4626
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4613..5464
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     complement(5670..5687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5921..5940)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6128..6149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6164..6169,6170..6187,6188..6194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6202..6218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6207..6229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6226..6242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6226..6242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6837..6863
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6873..6890)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6873..6889)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6882..6904)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(7226..7231,7232..7249,7250..7256)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7310..7990
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     promoter        join(8036..8041,8042..8059,8060..8066)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8120..8800
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(9483..9502)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9494..10309
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             9494..10309
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9567..9586)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa
       61 cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc
      121 cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc
      181 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg tactaactgt
      241 cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat
      301 aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa
      361 aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg
      421 ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt ctattcgctg
      481 cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct
      541 aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct
      601 ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat
      661 cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc
      721 gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt
      781 caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc
      841 ttgctgctgt tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc
      901 ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca
      961 ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta
     1021 ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc
     1081 ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga
     1141 ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg
     1201 gccagggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa
     1261 gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg
     1321 gatgctgccg acctgtatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc
     1381 ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac
     1441 ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc atgccggtgg cggcgagaac
     1501 ccgcactgcc acctgatgat ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct
     1561 cagtggttca agcggtacaa cggcaagacc ccggagaagg gcggggcaca gaagaccgaa
     1621 gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg catgggccga ccatgccaac
     1681 cgggcattag agcgggctgg ccacgacgcc cgcattgacc acagaacact tgaggcgcag
     1741 ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg tggtggagat ggaaggccgg
     1801 ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa cgcccagatc
     1861 atcgacttac aggaataccg ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa
     1921 atccagaggc atcaacgagt tagcggagca gatcgaaccg ctggcccaga gcatggcgac
     1981 actggccgac gaagcccggc aggtcatgag ccagacccag caggccagcg aggcgcaggc
     2041 ggcggagtgg ctgaaagccc agcgccagac aggggcggca tgggtggagc tggccaaaga
     2101 gttgcgggag gtagccgccg aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg
     2161 ggggtggcac tggaagctat ggctaaccgt gatgctggct tccatgatgc ctacggtggt
     2221 gctgctgatc gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg aggacggctc
     2281 gatctggctg cgcttggtgg cccgatgaag aacgacagga ctttgcaggc cataggccga
     2341 cagctcaagg ccatgggctg tgagcgcttc gatatcggcg tcagggacgc caccaccggc
     2401 cagatgatga accgggaatg gtcagccgcc gaagtgctcc agaacacgcc atggctcaag
     2461 cggatgaatg cccagggcaa tgacgtgtat atcaggcccg ccgagcagga gcggcatggt
     2521 ctggtgctgg tggacgacct cagcgagttt gacctggatg acatgaaagc cgagggccgg
     2581 gagcctgccc tggtagtgga aaccagcccg aagaactatc aggcatgggt caaggtggcc
     2641 gacgccgcag gcggtgaact tcgggggcag attgcccgga cgctggccag cgagtacgac
     2701 gccgacccgg ccagcgccga cagccgccac tatggccgct tggcgggctt caccaaccgc
     2761 aaggacaagc acaccacccg cgccggttat cagccgtggg tgctgctgcg tgaatccaag
     2821 ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg ctggccagca gatcgagcag
     2881 gcccagcggc agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag
     2941 cttagccgcc accggcgcac ggcgctggac gagtaccgca gcgagatggc cgggctggtc
     3001 aagcgcttcg gtgatgacct cagcaagtgc gactttatcg ccgcgcagaa gctggccagc
     3061 cggggccgca gtgccgagga aatcggcaag gccatggccg aggccagccc agcgctggca
     3121 gagcgcaagc ccggccacga agcggattac atcgagcgca ccgtcagcaa ggtcatgggt
     3181 ctgcccagcg tccagcttgc gcgggccgag ctggcacggg caccggcacc ccgccagcga
     3241 ggcatggaca ggggcgggcc agatttcagc atgtagtgct tgcgttggta ctcacgcctg
     3301 ttatactatg agtactcacg cacagaaggg ggttttatgg aatacgaaaa aagcgcttca
     3361 gggtcggtct acctgatcaa aagtgacaag ggctattggt tgcccggtgg ctttggttat
     3421 acgtcaaaca aggccgaggc tggccgcttt tcagtcgctg atatggccag ccttaacctt
     3481 gacggctgca ccttgtcctt gttccgcgaa gacaagcctt tcggccccgg caagtttctc
     3541 ggtgactgat atgaaagacc aaaaggacaa gcagaccggc gacctgctgg ccagccctga
     3601 cgctgtacgc caagcgcgat atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa
     3661 gttctggctg accgacgacg aatacgaggc gctgcgcgag tgcctggaag aactcagagc
     3721 ggcgcagggc gggggtagtg accccgccag cgcctaacca ccaactgcct gcaaaggagg
     3781 caatcaatgg ctacccataa gcctatcaat attctggagg cgttcgcagc agcgccgcca
     3841 ccgctggact acgttttgcc caacatggtg gccggtacgg tcggggcgct ggtgtcgccc
     3901 ggtggtgccg gtaaatccat gctggccctg caactggccg cacagattgc aggcgggccg
     3961 gatctgctgg aggtgggcga actgcccacc ggcccggtga tctacctgcc cgccgaagac
     4021 ccgcccaccg ccattcatca ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa
     4081 cggcaagccg tggctgacgg cctgctgatc cagccgctga tcggcagcct gcccaacatc
     4141 atggccccgg agtggttcga cggcctcaag cgcgccgccg agggccgccg cctgatggtg
     4201 ctggacacgc tgcgccggtt ccacatcgag gaagaaaacg ccagcggccc catggcccag
     4261 gtcatcggtc gcatggaggc catcgccgcc gataccgggt gctctatcgt gttcctgcac
     4321 catgccagca agggcgcggc catgatgggc gcaggcgacc agcagcaggc cagccggggc
     4381 agctcggtac tggtcgataa catccgctgg cagtcctacc tgtcgagcat gaccagcgcc
     4441 gaggccgagg aatggggtgt ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg
     4501 agcaaggcca actatggcgc accgttcgct gatcggtggt tcaggcggca tgacggcggg
     4561 gtgctcaagc ccgccgtgct ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa
     4621 gcctaagaac aagcacagcc tcagccacgt ccggcacgac ccggcgcact gtctggcccc
     4681 cggcctgttc cgtgccctca agcggggcga gcgcaagcgc agcaagctgg acgtgacgta
     4741 tgactacggc gacggcaagc ggatcgagtt cagcggcccg gagccgctgg gcgctgatga
     4801 tctgcgcatc ctgcaagggc tggtggccat ggctgggcct aatggcctag tgcttggccc
     4861 ggaacccaag accgaaggcg gacggcagct ccggctgttc ctggaaccca agtgggaggc
     4921 cgtcaccgct gatgccatgg tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg
     4981 ggcagaggtc gatagtggtg gggcgctcaa gcacatacag gactgcatcg agcgcctttg
     5041 gaaggtatcc atcatcgccc agaatggccg caagcggcag gggtttcggc tgctgtcgga
     5101 gtacgccagc gacgaggcgg acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca
     5161 ggccgtcatg ggtggcggcc agcatgtgcg catcagcatg gacgaggtgc gggcgctgga
     5221 cagcgaaacc gcccgcctgc tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac
     5281 cggcaaggct tccatagata ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc
     5341 gaccatgcgc aagcgccgcc agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg
     5401 ctggacggta accgagttcg cggcgggcaa gtacgacatc acccggccca aggcggcagg
     5461 ctgacccccc ccactctatt gtaaacaaga catttttatc ttttatattc aatggcttat
     5521 tttcctgcta attggtaata ccatgaaaaa taccatgctc agaaaaggct taacaatatt
     5581 ttgaaaaatt gcctactgag cgctgccgca cagctccata ggccgctttc ctggctttgc
     5641 ttccagatgt atgctcttct ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5701 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5761 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5821 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5881 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5941 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6001 gcctttctcc cttcgggaag cgtggcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6061 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6241 accatgatta cgccaagctt gcaggtagct tgcagtgggc ttacatggcg atagctagac
     6301 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     6361 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     6421 tctagattta agaaggagat atacatatga tgcccacggg gaaagtcaaa ttttacgatg
     6481 aagataaggg ctttggcttt attagcagtg atgatgggca ggaagtgttc ttgcacgctt
     6541 ccgcactccc atcgggcgtc gctggtgtta aagctggcac tcggctcgag tttggcatcg
     6601 cggatggtaa acgcggggcg caggcattgt cggcgcgtat tctggatgca cctccatccc
     6661 tcgctcgcat gagtcgcaaa ccggcggacg acatggccgt tatcgtcgaa gacttggtca
     6721 aagtcctgga tggtatcggc acgggtctga agcgcgggcg gtatcctgag gacagtcacg
     6781 gccgtaaaat cgccgctttg ctccgtcgtg tggcagaaga actcgacgcc ggtggttatc
     6841 catacgacgt cccggactat gcttgagaat tcactggccg tcgttttaca acgtcgtgac
     6901 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6961 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7021 ggcgaatgtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct
     7081 gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct
     7141 cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgtatc cagtcactat
     7201 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     7261 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     7321 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     7381 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     7441 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     7501 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     7561 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     7621 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     7681 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     7741 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     7801 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     7861 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     7921 gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc tagaggatcc
     7981 ggcttactaa cgtacgtatc cagtcactat ggcggccgca ttaggcaccc caggctttac
     8041 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
     8101 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     8161 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     8221 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     8281 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     8341 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     8401 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     8461 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     8521 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     8581 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     8641 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     8701 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     8761 gtggcaggcg gggcgtaatc tagaggatcc ggcttactaa cgtacgctta tgggtgcctt
     8821 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     8881 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     8941 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     9001 tcccgcaaac gaccccaaac aggaaacgca gctgtaatcc gggcagcgca acggaacatt
     9061 catcagtgta aaaatggaat caataaagcc ctgcgcagcg cgcagggtca gcctgaatac
     9121 gcgtttaatg accagcacag tcgtgatggc aaggtcagaa tagcgctgag gtctgcctcg
     9181 tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt
     9241 gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt
     9301 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc
     9361 agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat
     9421 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     9481 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
     9541 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     9601 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     9661 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     9721 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     9781 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
     9841 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     9901 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     9961 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
    10021 acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca
    10081 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
    10141 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
    10201 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
    10261 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
    10321 taattggttg taacactggc agagcattac gctgacttga cgggacggcg gctttgttga
    10381 ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac
    10441 cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc
    10501 atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag
    10561 tcagcaacac cttcttcacg aggcagacct cagcgctatt ctgaccttgc catcacgact
    10621 gtgctggtca ttaaacgcgt attcaggctg accctgcgcg ctgcgcaggg ctttattgat
    10681 tccattttta cactgatgaa tgttccgttg cgctgcccgg attacagctg aaagcgacca
    10741 ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc
    10801 attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
    10861 ccataagcgc agcgaatgcc
//
