LOCUS       sequence-417345-        8285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UBI:fLUC (Firefly luciferase with Ubiquitin promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8285)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSW027
FEATURES             Location/Qualifiers
     source          1..8285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    76..100
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      192..823
                     /label=oriV
                     /note="incP origin of replication"
     promoter        910..1014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1015..1083,1084..1875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1233..1252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2046..2634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2535..2554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2970..4118
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4274..4298
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4346..4598)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4611..6263)
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     6179..6197
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gtggggccca ctgcatccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
       61 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
      121 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tcagatcagg
      181 atctcctttg cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
      241 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
      301 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
      361 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
      421 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
      481 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
      541 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
      601 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
      661 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
      721 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
      781 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
      841 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
      901 caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      961 ctcatgagac aataaccttg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ttctcgcggt
     1741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2641 agcaacgcgg cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg
     2701 acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc
     2761 acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca
     2821 agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt
     2881 atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt
     2941 gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca
     3001 ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca
     3061 ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta
     3121 cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg
     3181 ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga
     3241 ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg
     3301 caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca
     3361 aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc
     3421 caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg
     3481 cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg
     3541 tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc
     3601 gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct
     3661 ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga
     3721 cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc
     3781 gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg
     3841 aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca
     3901 agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc
     3961 cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt
     4021 ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg
     4081 cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg
     4141 ctgggggttc agcagccagc gcctgatctg gggaaccctg tggttggcat gcacatacaa
     4201 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
     4261 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaaccact
     4321 tcgtgcagaa gacaattgca gcgtcgatct agtaacatag atgacaccgc gcgcgataat
     4381 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
     4441 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
     4501 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
     4561 aatcttaaga aactttattg ccaaatgttt gaacgatctg cttgacaagc ttacaatttg
     4621 gactttccgc ccttcttggc ctttatgagg atctctctga tttttcttgc gtcgagtttt
     4681 ccggtaagac ctttcggtac ttcgtccaca aacacaactc ctccgcgcaa ctttttcgcg
     4741 gttgttactt gactggcgac gtaatccacg atctcttttt ccgtcatcgt ctttccgtgc
     4801 tccaaaacaa caacggcggc gggaagttca ccggcgtcat cgtcgggcag acctgccacg
     4861 cccgcgtcga agatgttggg gtgttgtaac aatatcgatt ccaattcagc gggggccacc
     4921 tgatatcctt tgtatttaat taaagacttc aagcggtcaa ctatgaagaa gtgttcgtcc
     4981 tcgtcccagt aagctatgtc tccagaatgt agccatccat ccttgtcaat caaggcgttg
     5041 gtcgcttccg gattgtttac ataaccggac ataatcatag gtcctctgac acataattcg
     5101 cctctctgat taacgcccag cgttttcccg gtatccagat ccacaacctt cgcttcaaaa
     5161 aatggaacaa ctttaccgac cgcgcccggt ttatcatccc cctcgggtgt aatcagaata
     5221 gctgatgtag tctcagtgag cccatatcct tgtcgtatcc ctggaagatg gaagcgtttt
     5281 gcaaccgctt ccccgacttc tttcgaaaga ggtgcgcccc cagaagcaat ttcgtgtaaa
     5341 ttagataaat cgtatttgtc aatcagagtg cttttggcga agaatgaaaa tagggttggt
     5401 actagcaacg cactttgaat tttgtaatcc tgaagggatc gtaaaaacag ctcttcttca
     5461 aatctataca ttaagacgac tcgaaatcca catatcaaat atccgagtgt agtaaacatt
     5521 ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag tatccggaat gatttgattg
     5581 ccaaaaatag gatctctggc atgcgagaat ctgacgcagg cagttctatg cggaagggcc
     5641 acacccttag gtaacccagt agatccagag gaattcatta tcagtgcaat tgttttgtca
     5701 cgatcaaagg actctggtac aaaatcgtat tcattaaaac cgggaggtag atgagatgtg
     5761 acgaacgtgt acatcgactg aaatccctgg taatccgttt tagaatccat gataataatt
     5821 ttctggatta ttggtaattt tttttgcacg ttcaaaattt tttgcaaccc ctttttggaa
     5881 acaaacacta cggtaggctg cgaaatgttc atactgttga gcaattcacg ttcattataa
     5941 atgtcgttcg cgggcgcaac tgcaactccg ataaataacg cgcccaacac cggcataaag
     6001 aattgaagag agttttcact gcatacgacg attctgtgat ttgtattcag cccatatcgt
     6061 ttcatagctt ctgccaaccg aacggacatt tcgaagtatt ccgcgtacgt gatgttcacc
     6121 tcgatatgtg catctgtaaa agcaattgtt ccaggaacca gggcgtatct cttcatagcc
     6181 ttatgcagtt gctctccagc ggttccatcc tctagaggat agaatggcgc cgggcctttc
     6241 tttatgtttt tggcgtcctc cattcctgca gaagtaacac caaacaacag ggtgagcatc
     6301 gacaaaagaa acagtaccaa gcaaataaat agcgtatgaa ggcagggcta aaaaaatcca
     6361 catatagctg ctgcatatgc catcatccaa gtatatcaag atcaaaataa ttataaaaca
     6421 tacttgttta ttataataga taggtactca aggttagagc atatgaatag atgctgcata
     6481 tgccatcatg tatatgcatc agtaaaaccc acatcaacat gtatacctat cctagatcga
     6541 tatttccatc catcttaaac tcgtaactat gaagatgtat gacacacaca tacagttcca
     6601 aaattaataa atacaccagg tagtttgaaa cagtattcta ctccgatcta gaacgaatga
     6661 acgaccgccc aaccacacca catcatcaca accaagcgaa caaaaagcat ctctgtatat
     6721 gcatcagtaa aacccgcatc aacatgtata cctatcctag atcgatattt ccatccatca
     6781 tcttcaattc gtaactatga atatgtatgg cacacacata cagatccaaa attaataaat
     6841 ccaccaggta gtttgaaaca gaattctact ccgatctaga acgaccgccc aaccagacca
     6901 catcatcaca accaagacaa aaaaaagcat gaaaagatga cccgacaaac aagtgcacgg
     6961 catatattga aataaaggaa aagggcaaac caaaccctat gcaacgaaac aaaaaaaatc
     7021 atgaaatcga tcccgtctgc ggaacggcta gagccatccc aggattcccc aaagagaaac
     7081 actggcaagt tagcaatcag aacgtgtctg acgtacaggt cgcatccgtg tacgaacgct
     7141 agcagcacgg atctaacaca aacacggatc taacacaaac atgaacagaa gtagaactac
     7201 cgggccctaa ccatggaccg gaacgccgat ctagagaagg tagagagggg ggggggggga
     7261 ggacgagcgg cgtaccttga agcggaggtg ccgacgggtg gatttggggg agatctggtt
     7321 gtgtgtgtgt gcgctccgaa caacacgagg ttggggaaag agggtgtgga gggggtgtct
     7381 atttattacg gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga atcccccgta
     7441 gctgccgtgc cgtgagagga ggaggaggcc gcctgccgtg ccggctcacg tctgccgctc
     7501 cgccacgcaa tttctggatg ccgacagcgg agcaagtcca acggtggagc ggaactctcg
     7561 agaggggtcc agaggcagcg acagagatgc cgtgccgtct gcttcgcttg gcccgacgcg
     7621 acgctgctgg ttcgctggtt ggtgtccgtt agactcgtcg acggcgttta acaggctggc
     7681 attatctact cgaaacaaga aaaatgtttc cttagttttt ttaatttctt aaagggtatt
     7741 tgtttaattt ttagtcactt tattttattc tattttatat ctaaattatt aaataaaaaa
     7801 actaaaatag agttttagtt ttcttaattt agaggctaaa atagaataaa atagatgtac
     7861 taaaaaaatt agtctataaa aaccattaac cctaaaccct aaatggatgt actaataaaa
     7921 tggatgaagt attatatagg tgaagctatt tgcaaaaaaa aaggagaaca catgcacact
     7981 aaaaagataa aactgtagag tcctgttgtc aaaatactca attgtccttt agaccatgtc
     8041 taactgttca tttatatgat tctctaaaac actgatatta ttgtagtact atagattata
     8101 ttattcgtag agtaaagttt aaatatatgt ataaagatag ataaactgca cttcaaacaa
     8161 gtgtgacaaa aaaaatatgt ggtaattttt tataacttag acatgcaatg ctcattatct
     8221 ctagagaggg gcacgaccgg gtcacgctgc acctccggat ccgaattcgg cattgtcttc
     8281 acaga
//
