LOCUS       sequence-417362-        3060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL0_166 [Taad(9)]
FEATURES             Location/Qualifiers
     source          1..3060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(820..842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    831..847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(855..861,862..879,880..885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1038..1055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1209..1797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1289..1308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1968..2759,2760..2828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2591..2610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2829..2933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3001..3019
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccc tagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga caggcgacag
      481 attagcgtgc gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc
      541 tcgtctcgtc tccgaggtct cagcttaaaa attgaaaaaa tggtggaaac acttttttca
      601 atttttttgt tttattattt aatatttggg aaatattcat tctaattggt aatcagattt
      661 tagaagttta aacgcttgag accaccccag gatacataga ttaccacaac tccgagccct
      721 tccacctagc gtgctaacca cgcacgctat tgttgtatat cagttctgga ccagcgagct
      781 gtgctgcgac tcgtggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      961 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1021 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1321 ctcgtgcgct ctcctgttcc gaccctgtcg cttaccggat acctgtccgc ctttctccct
     1381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1621 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2101 ataccgcgag atccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2221 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2281 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2341 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2401 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2461 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2521 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2581 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2641 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2701 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2761 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2821 atactcatac tctacctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2881 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2941 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3001 aataggcgta tcacgaggcc ctttcatctc gcgcgtttcg gtgcacgagg ccctttcatc
//
