LOCUS       sequence-417382-        3311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 plasmid, barcoded ORI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Gilbert C, Lee H
  TITLE     POSSUM Expansion Pack
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGL1_108
FEATURES             Location/Qualifiers
     source          1..3311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(136..951)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(136..951)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     859..878
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     943..962
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1072..1088
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1072..1088
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1089..1135
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1249..1271
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1263..1280
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1264..1280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2535..2551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2535..2551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2548..2570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2723..3311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2803..2822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctctat tcaacaaagc cgccgtcccg
       61 tcaagtcagc gtaaatgggt agggggcttc aaatcgtccg ctctgccagt gttacaacca
      121 attaacaaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      181 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
      241 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
      301 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
      361 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
      421 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
      481 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
      541 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
      601 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
      661 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
      721 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
      781 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
      841 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
      901 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct
      961 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     1021 tgcaatgtaa catcagagat tttgagacac aacgtggctt tcccccgccg ctctagaact
     1081 agtggatcca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1141 gtttgtcgca ttatacgaga cgtccaggtt gggatacctg aaacaaaacc catcgtacgg
     1201 ccaaggaagt ctccaataac tgtgatccac cacaagcgcc agggttttcc cagtcacgac
     1261 gttgtaaaac gacggccagt catgcataat ccgcacgcat ctggaataag gaagtgccat
     1321 tccgcctgac cttagcgtgc gaacagcacg ctattgttgt atcattactc gcatccattc
     1381 tcaggctgtc tcgtctcgtc tccgagctct tcaatgagtc agggtcacgc gtaggacgcg
     1441 tcgagtaggg tcattgagag ggatgctttg ggttccgcag ccacacgccg tgtgaagctg
     1501 gggagggcgc gccgcattca cttcttttct atataaatat gagcgaagcg aataagcgtc
     1561 ggaaaagcag caaaaagttt cctttttgct gttggagcat gggggttcag ggggtgcagt
     1621 atctgacgtc aatgccgagc gaaagcgagc cgaagggtag catttacgtt agataacccc
     1681 ctgatatgct ccgacgcttt atatagaaaa gaagattcaa ctaggtaaaa tcttaatata
     1741 ggttgagatg ataaggttta taaggaattt gtttgttcta atttttcact cattttgttc
     1801 taatttcttt taacaaatgt tctttttttt ttagaacagt tatgatatag ttagaatagt
     1861 ttaaaataag gagtgagaaa aagatgaaag aaagatatgg aacagtctat aaaggctctc
     1921 agaggctcat agacgaagaa agtggagaag tcatagaggt agacaagtta taccgtaaac
     1981 aaacgtctgg taacttcgta aaggcatata tagtgcaatt aataagtatg ttagatatga
     2041 ttggcggaaa aaaacttaaa atcgttaact atatcctaga taatgtccac ttaagtaaca
     2101 atacaatgat agctacaaca agagaaatag caaaagctac aggaacaagt ctacaaacag
     2161 taataacaac acttaaaatc ttagaagaag gaaatattat aaaaagaaaa actggagtat
     2221 taatgttaaa ccctgaacta ctaatgagag gcgacgacca aaaacaaaaa tacctcttac
     2281 tcgaatttgg gaactttgag caagaggcaa atgaaataga ttgacctccc aataacacca
     2341 cgtagttatt gggaggtcaa tctatgaaat gcgattaagg gccggcccgc tagcatgaag
     2401 agcaccccag gatacataga ttaccacaac tccgagccct tccacctagc gtgctaacca
     2461 cgcacgctat tgttgtatag gctaggtgga ggctcagtga tgataagtct gcgatggtgg
     2521 atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac
     2581 aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta
     2641 tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3121 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3301 aaggatctca a
//
