LOCUS       sequence-417383-       11092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), 
            with BFP selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li 
            JK, Lister R
  TITLE     A modular dCas9-based recruitment platform for combinatorial 
            epigenome editing.
  JOURNAL   Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108.
  PUBMED    38000387
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108."
COMMENT     Sequence Label: LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP
            (dCas...
FEATURES             Location/Qualifiers
     source          1..11092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        213..712
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(248..271)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     645..664
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             744..764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             765..791
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             795..821
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             825..851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(885..911,912..914,915..3398,3399..3404,3405..3470,
                     3471..3473,3474..4988)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(885..911,912..914,915..3401,3402..3404,3405..4988)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             885..4988
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5025..5045
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5085..5105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5127..5165
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             5235..5270
                     /label=SnoopTag
     CDS             5337..5393
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5463..5519
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5586..5642
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5709..5765
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5832..5888
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5949..5993
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6018..6038
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6058..6077)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6102..6149
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6395..7573
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6626..7564
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7521..7541
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7580..7589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7586..8296
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     intron          8338..8567
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     8550..8569
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    8712..8833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8749..8768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8935..8951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8935..8951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8948..8970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8959..8975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8983..8989,8990..9007,9008..9013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9028..9049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9166..9183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9337..9925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9417..9436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10096..10887,10888..10956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10719..10738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10957..11061)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ttaattaata ccgggtaggg gaggcgcttt tcccaaggca
      241 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      301 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      361 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
      421 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
      481 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
      541 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
      601 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
      661 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cggcggccgc
      721 acctgcagcc caagcttacc atgccaaaga agaagcggaa ggtctacccc tatgacgtgc
      781 ctgattacgc cgcttatcct tacgatgtgc ccgactatgc agcctaccca tacgatgtgc
      841 ccgactacgc cgcaatggcc ggtatccacg gagtcccagc agccatggac aagaagtact
      901 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
      961 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga
     1021 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa
     1081 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct
     1141 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt
     1201 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg
     1261 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta
     1321 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg
     1381 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta
     1441 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag
     1501 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca
     1561 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac
     1621 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac
     1681 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt
     1741 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc
     1801 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg
     1861 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga
     1921 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg
     1981 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca
     2041 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg
     2101 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc
     2161 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat
     2221 ttcggatacc ctactatgtg ggccccctcg cccggggaaa ttccagattc gcgtggatga
     2281 ctcgcaaatc agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg
     2341 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa
     2401 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca
     2461 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga
     2521 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag
     2581 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc
     2641 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact
     2701 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt
     2761 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca
     2821 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac
     2881 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg
     2941 atggatttgc caacaggaac ttcatgcagt tgatccatga tgactctctc acctttaagg
     3001 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta
     3061 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg
     3121 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag
     3181 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag
     3241 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc
     3301 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc
     3361 aggaactgga catcaatcgg ctctccgact acgacgtgga tgcaatcgtg ccccagtctt
     3421 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga
     3481 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc
     3541 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag
     3601 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc
     3661 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa
     3721 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt
     3781 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg
     3841 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat
     3901 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg
     3961 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt
     4021 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa
     4081 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg
     4141 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct
     4201 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt
     4261 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg
     4321 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca
     4381 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag
     4441 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc
     4501 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc
     4561 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca
     4621 aagggtctcc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc
     4681 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta
     4741 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg
     4801 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt
     4861 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca
     4921 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg
     4981 gtggagacag cagggctgac gggggaggat cagggggagg gtcccccaaa aagaaacgca
     5041 aagtgaccgg caagaaaacc agcggccagg ccggacaggc ctctcctaag aaaaaaagaa
     5101 aagtgggacg cgctggcggc ggaagcgccc acatcgtgat ggtggacgcc tacaagccga
     5161 cgaagggaag cgggtccggt ggaagtggct ccggatctgg aggttctggc agcggaggta
     5221 gcggcagtgg cgggaaactg ggcgatattg aatttattaa agtgaacaaa gggtcgggaa
     5281 gtggcggcag cggaagtggg agtggaggga gcggttctgg cggttccggc agtggagagg
     5341 agcttctgag caaaaactat cacctcgaaa acgaggttgc gcgactgaag aaaggttccg
     5401 gctccaacgg tggaagtggc tccggatctg gaggttctgg cagcggaggt agcggcagtg
     5461 gcgaagagct ccttagtaag aactatcatc tggaaaatga ggtagcgcgc ttaaagaaag
     5521 ggtcgggaag tggcggcagc ggaagtggga gtggagggag cggttctggc ggttccggca
     5581 gtggagagga gttgctgtct aagaactacc acttagaaaa cgaagtcgca cggctaaaaa
     5641 aaggttccgg ctccggcggc tccggttctg gaagcggggg ctcgggatca ggtggatctg
     5701 gatcaggaga ggaattgctt tccaaaaact accaccttga gaatgaggtg gccaggttaa
     5761 agaaggggag cggctcgggg ggtagtggat cggggtcggg cgggtcagga agcggtggta
     5821 gcggatctgg ggaggagctg ctctcgaaga attaccattt ggagaacgaa gtggcgagac
     5881 taaagaaggg aagcggtagt tccggttctg gaagcggggg ctcgggatca ggtggatctg
     5941 gatcaggagg cctgaacgat atttttgaag cgcagaaaat tgaatggcat gaaggcggtg
     6001 gttccggagg aggtagtccg aagaagaaac gtaaggtcgg atgaggggga ggctaactga
     6061 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6121 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6181 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6241 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     6301 aggccctgcc atagtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     6361 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     6421 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     6481 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     6541 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     6601 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     6661 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     6721 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     6781 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     6841 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     6901 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     6961 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     7021 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     7081 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     7141 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     7201 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     7261 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     7321 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     7381 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     7441 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     7501 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     7561 tcaggtgtcg tgacgtacgg ccaccatggt gtctaagggc gaagagctga ttaaggagaa
     7621 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     7681 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     7741 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     7801 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     7861 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     7921 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     7981 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     8041 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     8101 ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa
     8161 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     8221 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     8281 ggggcacaag cttaattgat ggaattaatt cgctgtctgc gagggccagc tgttggggtg
     8341 agtactccct ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg
     8401 aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct
     8461 ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc
     8521 catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca
     8581 ggtccaactg caggtcgaga taactgatca taatcagcca taccacattt gtagaggttt
     8641 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8701 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8821 tcaatgtatc ttaatgcgtc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8881 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8941 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9001 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10081 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10141 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10201 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10261 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10321 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10381 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10441 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10501 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10561 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10621 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10681 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10741 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10801 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10861 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10921 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10981 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11041 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
